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Danny Bavli Elishai Ezra Daniel Kitsberg Margarita Vosk-Artzi Shashi K. Murthy Yaakov Nahmias 《Biomicrofluidics》2016,10(2)
Cell-cell interactions play a key role in regeneration, differentiation, and basic tissue function taking place under physiological shear forces. However, current solutions to mimic such interactions by micro-patterning cells within microfluidic devices have low resolution, high fabrication complexity, and are limited to one or two cell types. Here, we present a microfluidic platform capable of laminar patterning of any biotin-labeled peptide using streptavidin-based surface chemistry. The design permits the generation of arbitrary cell patterns from heterogeneous mixtures in microfluidic devices. We demonstrate the robust co-patterning of α-CD24, α-ASGPR-1, and α-Tie2 antibodies for rapid isolation and co-patterning of mixtures of hepatocytes and endothelial cells. In addition to one-step isolation and patterning, our design permits step-wise patterning of multiple cell types and empty spaces to create complex cellular geometries in vitro. In conclusion, we developed a microfluidic device that permits the generation of perfusable tissue-like patterns in microfluidic devices by directly injecting complex cell mixtures such as differentiated stem cells or tissue digests with minimal sample preparation. 相似文献
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K. S. Yakovlev D. A. Makarov E. S. Baskin 《Scientific and Technical Information Processing》2015,42(5):347-358
In the article we solve path planning task for an agent being multirotor unmanned aerial vehicle (multicopter). We propose an approach of estimating path geometry constraints based on UAV flight dynamics model and control constraints. Than we introduce a new path finding method which takes into consideration those geometry constraints and study this method both theoretically and empirically. 相似文献
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K.A. Cherevyk 《Russian Education & Society》2016,58(12):779-788
This article analyzes the situation surrounding the education of the disabled in Kyrgyzstan. We provide a detailed discussion of the national legal and regulatory framework that guarantees the rights of persons with disabilities to education. We describe the actual situation in the country concerning access to education for this section of the population as well as other factors that impact it. 相似文献
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Educational Psychology Review - In his 1973 article The Structure of ill structured problems, Herbert Simon proposed that solving ill-structured problems could be modeled within the same... 相似文献
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Christina Hamme Peterson Sean B. Hall Juleen K. Buser 《Counselor Education & Supervision》2016,55(2):80-94
Counselors (N = 911) reported the research skills needed for practice and subsequent research training needs. Findings indicate that counselors have a high need for research skills at work, but training needs differ significantly by counselor type. Recommendations include increasing emphasis on single‐case design, survey design, and widely available data analysis tools. 相似文献
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