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Philippe B. Katchunga Patrick N. Mirindi Antoine S. Kishabongo Justin C. Cikomola Socrate Bwanamdogo Jan Philippé Marijn M. Speeckaert Joris R. Delanghe 《Biochemia medica : ?asopis Hrvatskoga dru?tva medicinskih biokemi?ara / HDMB》2015,25(3):469-473
Introduction
Diagnosis and monitoring of diabetes mellitus in sub-Saharan Africa, based on blood analyses, are hampered by infrastructural and cultural reasons. The first aim of this study was to evaluate the diagnostic accuracy of glycated nail proteins for diabetes mellitus. The second aim was to compare the course of short- and long-term glycemic biomarkers after 6 months of antidiabetic treatment. These objectives should support our hypothesis that glycated nail proteins could be used as an alternative glycemic biomarker.Materials and methods
This case-control study consisted of 163 black diabetics and 67 non-diabetics of the South Kivu (Democratic Republic of Congo). Diagnostic accuracy of glycated nail proteins was evaluated using ROC curve analysis. At the start of the study, glycated nail protein concentrations were compared between diabetics and non-diabetics, using a nitro blue tetrazolium (NBT) colorimetric method. In a subgroup of 30 diabetics, concentrations of glycated nail proteins, fasting glucose (Accu-Chek® Aviva), serum fructosamine (NBT) and HbA1c (DCA-2000+®) were measured at start and after 6 months.Results
ROC analysis yielded an AUC of 0.71 (95% confidence interval (CI): 0.65-0.76) and a cut-off point of 3.83 μmol/g nail. Concentration of glycated nail proteins was significantly higher (P < 0.001) in diabetics in comparison with non-diabetics. After 6 months of antidiabetic treatment, a significant drop in the fasting glucose concentration (P = 0.017) and concentration of glycated nail proteins (P = 0.008) was observed in contrast to serum fructosamine and HbA1c.Conclusions
Measurement of glycated nail proteins could be used to diagnose and monitor diabetes mellitus in sub-Saharan Africa.Key words: fasting glucose concentration, fructosamine, hemoglobin A1c protein, nails, sub-Saharan Africa, diabetes mellitus 相似文献63.
Willem G. van Kernebeek Antoine W. de Schipper Geert J.P. Savelsbergh Huub M. Toussaint 《Measurement in physical education and exercise science》2018,22(2):129-137
In The Netherlands, the 4-Skills Scan is an instrument for physical education teachers to assess gross motor skills of elementary school children. Little is known about its reliability. Therefore, in this study the test–retest and inter-rater reliability was determined. Respectively, 624 and 557 Dutch 6- to 12-year-old children were analyzed for test re-test and inter-rater reliability. All tests took place within the school setting. The outcome measure was age-expected motor performance (in years). Results showed a small practice effect of .24 years for re-test sessions and assessment of motor skills was possible with acceptable precision (standard error of measurement = .67 years). Overall, intraclass correlation coefficient (ICC) was .93 (95% confidence interval: .92–.95) for test–retest reliability and .97 for inter-rater reliability. For the repeated measures, the smallest detectable change (SDC) was 1.84 and limits of agreement were –1.60 and 2.08 years. It can be concluded that the 4-Skills Scan is a reliable instrument to assess gross motor skills in elementary school children. 相似文献