Hydrolates and Gellan: An Eco-innovative Synergy for Safe Cleaning of Paper Artworks

The gellan hydrogel is used as a cleaning agent for paper artworks. The purposes of this work were to give the gellan hydrogel both a microbial resistance and a cytocidal action against the biodeteriogens using hydrolates. Broth microdilution assays were performed to identify the fungicidal action of 16 hydrolates vs 3 fungal strains. The study concerned the Minimal Fungicidal Concentration and pH, colour, and ultra-structural variations on all paper samples, by placing them in contact with GELYD (GELlan?+?hYDrolat). The in vitro tests showed that eight out of 16 hydrolates strongly inhibited fungal growth, but only two of them (Monarda fistulosa, Citrus aurantium subsp amara) showed in situ fungicidal action. Data indicate a new and interesting eco-innovative use of hydrolates, since GELYD is able not only to clean paper artworks, but also to kill the fungal cells present on paper that are potentially harmful for paper and operators.     

Incorrect order of draw could be mitigate the patient safety: a phlebotomy management case report

Procedures involving phlebotomy are critical for obtaining diagnostic blood specimens and represent a well known and recognized problem, probably among the most important issues in laboratory medicine. The aim of this report is to show spurious hyperkalemia and hypocalcemia due to inadequate phlebotomy procedure. The diagnostic blood specimens were collected from a male outpatient 45 years old, with no clinical complaints. The tubes drawing order were as follows: i) clot activator and gel separator (serum vacuum tube), ii) K₃EDTA, iii) a needleless blood gas dedicated-syringe with 80 I.U. lithium heparin, directly connected to the vacuum tube holder system. The laboratory testing results from serum vacuum tube and dedicated syringe were 4.8 and 8.5 mmol/L for potassium, 2.36 and 1.48 mmol/L for total calcium, respectively. Moreover 0.15 mmol/L of free calcium was observed in dedicated syringe. A new blood collection was performed without K₃EDTA tube. Different results were found for potassium (4.7 and 4.5 mmol/L) and total calcium (2.37 and 2.38 mmol/L) from serum vacuum tube and dedicated syringe, respectively. Also free calcium showed different concentration (1.21 mmol/L) in this new sample when compared with the first blood specimen. Based on this case we do not encourage the laboratory managers training the phlebotomists to insert the dedicated syringes in needle-holder system at the end of all vacuum tubes. To avoid double vein puncture the dedicated syringe for free calcium determination should be inserted immediately after serum tubes before EDTA vacuum tubes.     

Repeated sprint ability in young basketball players: one vs. two changes of direction (Part 2)

The aim of this study was to compare the training effects based on repeated sprint ability (RSA) (with one change of direction) with an intensive repeated sprint ability (IRSA) (with two changes of direction) on jump performance and aerobic fitness. Eighteen male basketball players were assigned to repeated sprint ability and intensive repeated sprint ability training groups (RSAG and IRSAG). RSA, IRSA, squat jump (SJ), countermovement jump (CMJ) and Yo-Yo intermittent recovery level 1 test were assessed before and after four training weeks. The RSA and IRSA trainings consisted of three sets of six sprints (first two weeks) and eight sprints (second two weeks) with 4-min sets recovery and 20-s of sprints recovery. Four weeks of training led to an overall improvement in most of the measures of RSA, but little evidence of any differences between the two training modes. Jump performance was enhanced: CMJ of 7.5% (P < 0.0001) and 3.1% (P = 0.016) in IRSAG and RSAG respectively. While SJ improved of 5.3% (P = 0.003) for IRSAG and 3.4% (P = 0.095) for RSAG. Conversely the Yo-Yo distance increased 21% (P = 0.301) and 34% (P = 0.017) in IRSAG and RSAG respectively. Therefore, short-term repeated sprint training with one/two changes of direction promotes improvements in both RSA and IRSA respectively but the better increase on jump performance shown a few changes on sprint and endurance performances.     

Seismic vulnerability of natural stone pinnacles on the Amalfi Coast in Italy

In the southern part of Italy, along the National Road 163 (the Amalfi Coastal road), and particularly close to Positano, some stone formations are present with a cylindrical shape (pinnacles) due to the erosion of the rock and the presence of sub-vertical cracking planes in the rocky slope. The landscape beauty and the dangerousness of these pinnacles, more than 10 m in height, leaning on the underlying road, requested a vulnerability study. The dynamic behavior of these natural solids is influenced by the notable mechanical inhomogeneity due to the nature of the rock, to the presence of diffused crack patterns and to the position along the slope. Some inspections have been performed to have a detailed survey of the pinnacles, using the Laser Scanner 3D technique and with the collaboration of geologist-mountain climbers; some rock specimens have also been taken to be analyzed and tested. The specific weight, the tensile (for bending) strength and the compressive strength, as well as the Young Modulus, considering various directions with respect to cracking planes, were analyzed during laboratory tests on the specimens sampled on site. Different structural modeling to analyze the seismic vulnerability of the pinnacles have been performed and compared: starting from simplified mechanical models of rigid blocks, passing through elastic analyses and finally to finite element (FEM) analyses. Numerical dynamic and static analyses, particularly, the modal dynamic analyses for the elastic continuum and the non-linear static analyses, considering both cracking and plasticity behavior of the rock have been performed. The analyses concerned the pinnacles both under the actual in-situ conditions and according to some strengthening interventions to evaluate the effectiveness and safety of the design.     

Control of PDE-ODE cascades with Neumann interconnections

We extend several recent results on full-state feedback stabilization and state estimation of PDE-ODE cascades, where the PDEs are either of heat type or of wave type, from the previously considered cases where the interconnections are of Dirichlet type, to interconnections of Neumann type. The Neumann type interconnections constrain the PDE state to be subject to a Dirichlet boundary condition at the PDE-ODE interface, and employ the boundary value of the first spatial derivative of the PDE state to be the input to the ODE. In addition to considering heat-ODE and wave-ODE cascades, we also consider a cascade of a diffusion-convection PDE with an ODE, where the convection direction is “away” from the ODE. We refer to this case as a PDE-ODE cascade with “counter-convection.” This case is not only interesting because the PDE subsystem is unstable, but because the control signal is subject to competing effects of diffusion, which is in both directions in the one-dimensional domain, and counter-convection, which is in the direction that is opposite from the propagation direction of the standard delay (transport PDE) process. We rely on the diffusion process to propagate the control signal through the PDE towards the ODE, to stabilize the ODE.     

Contamination of lithium heparin blood by K2-ethylenediaminetetraacetic acid (EDTA): an experimental evaluation

Introduction:

The contamination of serum or lithium heparin blood with ethylenediaminetetraacetic acid (EDTA) salts may affect accuracy of some critical analytes and jeopardize patient safety. The aim of this study was to evaluate the effect of lithium heparin sample contamination with different amounts of K₂EDTA.

Materials and methods:

Fifteen volunteers were enrolled among the laboratory staff. Two lithium heparin tubes and one K₂EDTA tube were collected from each subject. The lithium-heparin tubes of each subject were pooled and divided in 5 aliquots. The whole blood of K₂EDTA tube was then added in scalar amount to autologous heparinised aliquots, to obtained different degrees of K₂EDTA blood volume contamination (0%; 5%; 13%; 29%; 43%). The following clinical chemistry parameters were then measured in centrifuged aliquots: alanine aminotranspherase (ALT), bilirubin (total), calcium, chloride, creatinine, iron, lactate dehydrogenase (LD), lipase, magnesium, phosphate, potassium, sodium.

Results:

A significant variation starting from 5% K₂EDTA contamination was observed for calcium, chloride, iron, LD, magnesium (all decreased) and potassium (increased). The variation of phosphate and sodium (both increased) was significant after 13% and 29% K₂EDTA contamination, respectively. The values of ALT, bilirubin, creatinine and lipase remained unchanged up to 43% K₂EDTA contamination. When variations were compared with desirable quality specifications, the bias was significant for calcium, chloride, LD, magnesium and potassium (from 5% K₂EDTA contamination), sodium, phosphate and iron (from 29% K₂EDTA contamination).

Conclusions:

The concentration of calcium, magnesium, potassium, chloride and LD appears to be dramatically biased by even modest K₂EDTA contamination (i.e., 5%). The values of iron, phosphate, and sodium are still reliable up to 29% K₂EDTA contamination, whereas ALT, bilirubin, creatinine and lipase appear overall less vulnerable towards K₂EDTA contamination.     

Avoidance to wipe alcohol before venipuncture is not a source of spurious hemolysis

Background

It is still uncertain whether or not avoidance to let disinfectant alcohol dry at the site of venipuncture is a source of spurious hemolysis when drawing venous blood.

Methods:

In a consecutive series of 52 outpatients referred for routine laboratory testing, venous blood was drawn by direct venipuncture with (odd group) or without (pair group) wiping 70% isopropyl alcohol at the site of venipuncture. A 3.5 mL evacuated tube with clot activator and gel separator was drawn from a vein of the upper limb, serum was immediately separated with standard centrifugation and tested for potassium, lactate dehydrogenase (LD), aspartate aminotransferase (AST) and hemolysis index (HI) on Roche Cobas.

Results:

No specimen was discarded for unsatisfactory venipuncture. No differences for age and gender were observed between groups. As regards the four parameters investigated, no significant differences could be observed between patients in whom blood was drawn with or without letting the alcohol dry. It is also noteworthy that no sample in both groups exceeded the conventional sample rejection threshold of cell-free hemoglobin.

Conclusions:

The results of our prospective, randomized study attest that failure to wipe alcohol at the site of venipuncture should not be considered as a potential source of spurious hemolysis when drawing blood.     

企业网络组织及其理论基础

企业网络组织作为一种介于市场和企业之间的新的经济活动制度安排，近年来在理论和实践方面都得到了迅速发展，它的出现改变了企业是论的研究重心，甚至对整个经济产生了巨大的影响，本文阐述了企业网络组织的有关概念及其思想的渊源，并且从分工与专业化，交易成本，资源外取与共同管理，企业竞争和团队产生管理论角度探讨了企业的网络组织的理论基础。     

Stability and change of moral disengagement and its impact on aggression and violence in late adolescence

Stability and change of moral disengagement were examined in a sample of 366 adolescents from ages 14 to 20 years. Four developmental trajectories were identified: (a) nondisengaged group that started with initially low levels followed by an important decline, (b) normative group that started with initially moderate levels followed by a decline, (c) later desister group that started with initially high-medium levels followed by an increase from 14 to 16 years and an even steeper decline from 16 to 20 years, and (d) chronic group that started with and maintained medium-high levels. The results attest that adolescents who maintained higher levels of moral disengagement were more likely to show frequent aggressive and violent acts in late adolescence.     

Academic stratification and functional theory

This paper examines empirically a set of propositions on organizational stratification drawn from functional theory. The data consist of 38 departments of a large university which are treated as contextual units, as well as the responses of individual faculty to a survey. The findings present an interesting theoretical paradox: (1) functional arguments do not appear adequate to account for differences in income stratification among departments, but (2) they do explain participants' beliefs and judgments about the legitimacy of organizational reward systems. The implications are discussed, and a line of inquiry is suggested that would focus on the problem of establishing legitimacy with the environment(s) as a major source of organizational structure. It is suggested that organizational features act as symbolic devices that inform others that a given organization is a competent member of its set. In short, it is conforming to agreed upon definitions of the appropriate—most rational—structure for thattype of organization.