A deep vein thrombosis caused by 20209C>T mutation in homozygosis of the prothrombin gene in a Caucasian patient

Introduction:

Additional nucleotide substitutions in the 3′-untranslated region of prothrombin gene could explain some thrombotic events and also adverse pregnancy outcomes. We describe the first case of a homozygous 20209C>T mutation as the cause of deep vein thrombosis in a Spanish patient.

Case and methods:

The 56-year-old male patient with a partial tear of the Achilles tendon developed calf (tibial) deep vein thrombosis after immobilization and was treated with an anticoagulant. To determine if the deep vein thrombosis was of genetic origin, a peripheral blood DNA sample was analysed for the presence of the three most frequent mutations associated with thrombotic events: factor V Leiden (1691G>A), prothrombin (20210G>A) and methylene tetrahydrofolate reductase (677C>T). The presence or absence of the normal allele of prothrombin could not be determined using the PTH-FV-MTHFR StripAssay (Vienna Lab).

Results:

Comprehensive analysis showed that the patient had a variant interfering with the polymerase chain reaction product, we sequenced the entire prothrombin gene and found that the patient had a homozygous C>T mutation at position 20209; this interfered with the polymerase chain reaction product, which needs a C at this position to be able to bind to the wild-type probe present in the test strip.

Conclusion:

The homozygous 20209C>T mutation and the presence of the mutation 677C>T in heterozygosity explained the patient’s deep vein thrombosis because the combination of mutations would increase the risk of thrombosis. Suitable genetic counselling should be provided to the patient and first-degree relatives as it important to detect prothrombin gene variants that could increase risk for thrombotic events.     

Green intellectual capital and environmental product innovation: the mediating role of green social capital

Although the importance of intellectual capital has already been highlighted in the management literature, few articles have focused on the importance of its green counterpart, green intellectual capital. Nowadays, when environmental concerns cannot be ignored by firms, it is therefore time to explore the role of green intellectual capital in environmental improvement. For that reason, our research aims to explore the relationships between green intellectual capital components and the environmental performance of the firms. In particular, we try to show the importance of green organizational and social capital in the development of environmental product innovations, which are vital to the development and implementation of environmental strategies since, as scholars have pointed out, they generate some of the greatest environmental impacts. The empirical results show that green organizational capital has an indirect impact on environmental product innovation through green social capital, meaning that the latter is a significant mediator of that relationship, and at the same time emphasizing that informal relationships play a key role in the improvement of the environmental performance of the firm and, more specifically, in the development of environmental product innovations.     

Social capital as a driver of local knowledge exchange: a social network analysis

The objective of this paper is to take an in-depth look, from a network perspective, at the role that social capital plays in improving knowledge exchange between organizations. To do this, we consider the three dimensions of social capital: the structural dimension, as measured by the position of firms bridging structural holes; the relational dimension, as gauged by the level of trust and friendship in each relationship; and the cognitive dimension, as estimated by the presence of a shared culture. We also evaluate the level of organizational and human capital present in terms of the firms in the network. Empirical evidence has been gathered from the Madrid Science Park in Spain by applying the social network analysis methodology. The results show that the three dimensions of social capital play a significant role in increasing knowledge exchange in this network, while human and organizational capital differences are only significant for a sub-sample.     

Inducing chemotactic and haptotactic cues in microfluidic devices for three-dimensional in vitro assays

Microfluidic devices allow for the production of physiologically relevant cellular microenvironments by including biomimetic hydrogels and generating controlled chemical gradients. During transport, the biomolecules interact in distinct ways with the fibrillar networks: as purely diffusive factors in the soluble fluid or bound to the matrix proteins. These two main mechanisms may regulate distinct cell responses in order to guide their directional migration: caused by the substrate-bound chemoattractant gradient (haptotaxis) or by the gradient established within the soluble fluid (chemotaxis). In this work 3D diffusion experiments, in combination with ELISA assays, are performed using microfluidic platforms in order to quantify the distribution of PDGF-BB and TGF-β₁ across collagen and fibrin gels. Furthermore, to gain a deeper understanding of the fundamental processes, the experiments are reproduced by computer simulations based on a reaction-diffusion transport model. This model yields an accurate prediction of the experimental results, confirming that diffusion and binding phenomena are established within the microdevice.     

Globalisation in Foreign Language Teaching: Establishing Transatlantic Links in Higher Education

This article makes an explicit comparison between the reconfiguration of tertiary language education in Europe, the USA and Canada. It argues that, despite certain minor differences between continents, the same trends are operative across the Atlantic. It then examines each of them, illustrating how similar forces are at work in shaping higher education language teaching, how analogous policy frameworks are being employed to channel them and how parallel challenges and responses can be detected transatlantically. The conclusion is that unifying efforts and connecting initiatives has highly beneficial effects for advancing the application of higher education language policy worldwide.     

大运动量训练时自由式摔跤运动员应激激素变化的探讨

观察了大运动量训练期8名自由式摔跤运动员应激激素、焦虑水平及主观强度感的变化。研究过程中,每周安排11次大运动量训练,共进行2次实验,每次3个取样点(运动前、即刻和次日晨)。结果显示,两次取样时期次日晨ACTH水平较运动后即刻明显降低;C水平在第二次取样运动后即刻水平和次日晨水平明显高于运动前的,并且该时期运动后即刻C水平也明显高于第一次取样时期运动后即刻水平;E水平在第二次取样运动后即刻水平明显高于第一次取样运动后即刻的,且次日晨该激素水平也明显高于第一次取样次日晨的;血浆β-END水平在第二次取样运动后即刻水平明显高于运动前,也高于第一次取样运动后即刻,第二次取样次日晨该激素水平均低于运动前和运动后即刻水平,同时低于第一次取样次日晨的;血清NE水平变化与β-END水平变化类似;而RPE等级和焦虑水平在两次测试中呈现出运动后即刻水平高于运动前和次日晨的趋势,但不明显;RPE等级与诸多应激激素之间存在着弱相关关系。这预示着,在运动员身体机能评定工作中欲采用简单易行的显示身体机能的外部指标来表现不易测到的内部变化还需努力。     

The family caregiver after the institutionalization of the dependent elderly relative

The objective of this study was to assess the relationship between the institutionalization of a dependent elderly relative and the family caregiver’s mental health, quality of life and caregiving satisfaction. We present data from 140 family caregivers caring for elderly family members, of whom 77 continued to live with family members and 63 had been institutionalized. We evaluated the factors influencing the decision to institutionalize the dependent relative, the effect of institutionalization on the caregiver’s psychological well-being and quality of life and the impact of strategies to find the meaning and cope with the relative’s placement on the caregiver’s emotional health and quality of life. Care does not end after institutionalization, but the stressors the caregiver faces change. Our results suggest that finding meaning increases satisfaction and reduces feelings of overload. They show that after institutionalization, stressors affecting caregivers are partly relieved, and that finding meaning increases the caregiver’s satisfaction, while decreasing the degree of overload. Our data, further, indicates that a good relationship and cooperation between the staff of the institution and the family are important to improve the caregivers’ emotional health and quality of life, and consequently, the quality of life of the dependent elderly relative.