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BISp-Informationen
Informationen des Bundesinstituts für Sportwissenschaft (BISp) 相似文献13.
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Katherine Hart LaVail Ashley E. Anker Amber Reinhart Thomas Hugh Feeley 《Communication Studies》2013,64(1):46-69
The use of shared experiences and an accurate representation of real life situations is critical to effective speech education. This essay examines the use of role playing and principles of learning applied to the modern speech classroom. 相似文献
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Kai Reinhart 《Sportwissenschaft》2001,31(1):107-110
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A variety of methods have been used to introduce chemicals into a stream or to mix two or more streams of different compositions using microfluidic devices. In the following paper, the introduction of cryoprotective agents (CPAs) used during cryopreservation of cells in order to protect them from freezing injuries and increase viability post thaw is described. Dimethylsulphoxide (DMSO) is the most commonly used CPA. We aim to optimize the operating conditions of a two-stream microfluidic device to introduce a 10% vol/vol solution of DMSO into a cell suspension. Transport behavior of DMSO between two streams in the device has been experimentally characterized for a spectrum of flow conditions (0.7 < Re < 10), varying initial donor stream concentrations, (1% vol/vol < Co < 15% vol/vol) and different flow rate fractions (0.23 < fq < 0.77). The outlet cell stream concentration is analyzed for two different flow configurations: one with the cell stream flowing on top of the DMSO-rich donor stream, and the other with the cell stream flowing beneath the heavy DMSO-laden stream. We establish a transition from a diffusive mode of mass transfer to gravity-influenced convective currents for Atwood numbers (At) in the range of (1.7 × 10−3 < At < 3.1 × 10−3) for the latter configuration. Flow visualization with cells further our understanding of the effect of At on the nature of mass transport. Cell motion studies performed with Jurkat cells confirm a high cell recovery from the device while underscoring the need to collect both the streams at the outlet of the device and suggesting flow conditions that will help us achieve the target DMSO outlet concentration for clinical scale flow rates of the cell suspension. 相似文献
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We develop an approximation for the probability of optically resolving two fluorescent labels on the backbone of a DNA molecule confined in a nanochannel in the Odijk regime as a function of the fluorescence wavelength, channel size, and the properties of the DNA (persistence length and effective width). The theoretical predictions agree well with equivalent data produced by Monte Carlo simulations of a touching wormlike bead model of DNA in a high ionic strength buffer. Although the theory is only strictly valid in the limit where the effective width of the nanochannel is small compared with the persistence length of the DNA, simulations indicate that the theoretical predictions are reasonably accurate for channel widths up to two-thirds of the persistence length. Our results quantify the conjecture that DNA barcoding has kilobase pair resolution-provided the nanochannel lies in the Odijk regime. 相似文献
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Fußball is the most popular sport in Germany. The reasons for this extraordinary status and success of association football in Germany are complex. In this article, answers are searched for in the context of the historical development of German society. After a review of the recent development of German football and football historiography, the paper focuses on two issues relating to the origins of German football. These are the transformation of football in the then dominant body culture of gymnastics, and secondly its impact on the German process of nation-building. Both processes are not only linked, but also related to the concept of manliness in German sport and society. This is the reason why women’s football in Germany was not accepted until the late 1960s in German society—and British as well. 相似文献
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