Osteoinduction by Ca-P biomaterials implanted into the muscles of mice

The osteoinduction of porous biphasic calcium phosphate ceramics (BCP) has been widely reported and documented, but little research has been performed on rodent animals, e.g., mice. In this study, we report osteoinduction in a mouse model. Thirty mice were divided into two groups. BCP materials (Sample A) and control ceramics (Sample B) were implanted into the leg muscle, respectively. Five mice in each group were killed at 15, 30, and 45 d after surgery. Sample A and Sample B were harvested and used for hematoxylin and eosin (HE) staining, immunohistochemistry (IHC) staining, and Alizarin Red S staining to check bone formation in the biomaterials. Histological analysis showed that no bone tissue was formed 15 d after implantation (0/5) in either of the two groups. Newly-formed bone tissues were observed in Sample A at 30 d (5/5) and 45 d (5/5) after implantation; the average amounts of newly-formed bone tissues were approximately 5.2% and 8.6%, respectively. However, we did not see any bone tissue in Sample B until 45 d after implantation. Bone-related molecular makers such as bone morphogenesis protein-2 (BMP-2), collagen type I, and osteopontin were detected by IHC staining in Sample A 30 d after implantation. In addition, the newly-formed bone was also confirmed by Alizarin Red S staining. Because this is the report of osteoinduction in the rodent animal on which all the biotechnologies were available, our results may contribute to further mechanism research.     

Preliminary study on the freeze-drying of human bone marrow-derived mesenchymal stem cells

Long-term preservation and easy transportation of human bone marrow-derived mesenchymal stem cells (hBM-MSCs) will facilitate their application in medical treatment and bioengineering. A pilot study on the freeze-drying of hBM-MSCs was carried out. hBM-MSCs were loaded with trehalose. The glass transition temperature of the freeze-drying suspension was measured to provide information for the cooling and primary drying experiment. After freeze-drying, various rehydration processes were tested. The highest recovery rate of hBM-MSCs was (69.33±13.08)%. Possible methods to improve freeze-drying outcomes are discussed. In conclusion, the present study has laid a foundation for the freeze-drying hBM-MSCs.     

九十年代目录学研究新进展

本文从宏观的角度，对我国９０年代目录学研究的成就及其特征进行了全面总结，并从基础理论、分支学科、书目情报工作、目录学史以及国外目录学研究５个方面，详尽剖析了目录学研究领域出现的新思想、新观点、新方法，在此基础上，指出了目录学研究存在的问题及其发展的方向。     

A martyr for modernity: Qiu Jin -- feminist warrior, and revolutionary

Qui Jin, at one level, was an oriental twentieth-century Judith, the mythical Jewish widow from Bethulia who cut off the head of Holofernes, the Assyrian general besieging the city, thus saving the Israelites from destruction. Qui Jin was, as Judith was, a self-reliant heroine who when others seemed 'helpless and demoralized undertook to save them single-handedly', or in her case virtually single-handedly. This, of course, was both her making and her unmaking. In Chinese terms the story of Qui Jin, like the story of Judith if less famous, less publicised, more recent, is the story of an icon at once central and at the same time marginal to tradition. She contradicted the most cherished customs on Confucian Chinese culture. She was a radical force who thrust her way to the centre of the concentric circles of customs surrounding this culture and was pushed back to the margins by conservatism. Nevertheless Qui Jin was not without success. She challenged a long-established mythology of exclusively masterful patriarchy - and created a counter myth of purposeful patriotic feminism. She was a counter-cultural icon who changed perceptions of Chinese femininity. She gave courage, confidence and purpose to those women who came after her and absorbed her ambitions for modern Chinese womanhood. For them she was a modern national heroine and a personification of a modern nation of equal men and women. For Qui Jin the body was an instrument of female revolution to be trained, strengthened and prepared for confrontation. As a revolutionary militant she was a failure; as a revolutionary talisman she was a success. For the Chinese women of the 1911 Revolution hers was an exemplary emancipatory story: subscribe, struggle, sacrifice. Patriotism through feminism is the purpose. Her heroism was firmly outside the historic patriarchal order. Her adulation is thus all the more remarkable because of the profound traditions she rejected, the controversial mannerisms she adopted, the uncompromising attitudes she embraced. She eschewed motherhood, abandoned marriage, dismissed femininity, and yet won acclaim in the most traditional of cultures. Qui Jin was hardly a cynosure of universal acclaim but she was admired, respected and emulated by radical Chinese women and men seeking a new society accommodating women. Her modern feminism struggled to overcome an ancient patriarchy. Here was her appeal. She exuded no moral ambiguity. Consequently, if she was demonized by the conventional; she was deified by the radical - and inspired them as the contemplated and attempted to construct the future. There is a point, of course, that should not be overlooked. Qui Jin, in fact, is not divorced from occidental culture and political iconography. Qui Jin is closely associated with the attitudes, aspirations and fantasies of modern Western feminism. As Margarita Stocker observes, a 'romantic heroine, angry feminist, radical, activist is one example of a pervasive figure', in modern Western cultural mythology 'a figure we may sum up as the Woman with a Gun'. Force, that potent means to power, is available to the gun user irrespective of age of sex, with a resulting 'crucial alteration in the sexual politics of violence'. The Woman with a Gun can now be emphatically heroic - without duplicity, without deceitfulness, without subterfuge. Moral ambiguity in action has been abandoned. She becomes an unambiguous potent force - an armed woman faces an armed man on equal terms - physically, psychologically, morally. Equality offers the legal right and responsibility to kill in the name of patriotism. Modern culture has just caught up with Qui Jin.     

Effects of three fluid resuscitation methods on apoptosis of visceral organs in rats with hemorrhagic shock

INTRODUCTION Hemorrhagic shock is a common clinic emer- gency case. Successful treatment includes surgical control of hemorrhage and restoration of tissue per- fusion. Current guidelines for presurgical treatment of patients with hemorrhagic shock recommend rapid volume resuscitation to normal blood pressure as quickly as possible. The practice is controversial because aggressive restoration of intravascular vol- ume and rapid increasing of blood pressure before securing hemostasis may ex…     

促排卵后的不同步双侧异位妊娠(英文)

研究目的:报道一例不同步双侧异位妊娠,并回顾相关文献,总结规律。创新要点:第一次报道了不同步的双侧异位妊娠。研究方法:对促排卵后行宫腔内人工授精病人发生非同步双侧输卵管妊娠的病例进行报告,辅以人绒毛膜促性腺激素(hCG)值波动曲线、超声图像以及病理切片加以阐述;同时回顾2008年以来关于双侧异位妊娠的文献,并分析病例特征。重要结论:促排卵后可能会提高双侧异位妊娠风险,一侧异位妊娠发生后,需要注意对侧是否也存在异位妊娠。     

论和平时期军事新闻传媒对中国国家形象的建构和传播

和平时期的军事新闻传播有着广泛的受众群体。为了能对和平时期军事新闻传播的功能有一个全方位的把握,需要对它作一个多维的剖析。本文主要对和平时期军事新闻传媒与中国国家形象塑造作了一个较深入的理论探讨。通过文中的论述,作者意在让受众对和平时期军事新闻传媒是中国国家形象建构和传播的最为有效的手段之一有一个充分的认识。