Effects of purposeful soccer heading on circulating small extracellular vesicle concentration and cargo

BackgroundConsidering the potential cumulative effects of repetitive head impact (HI) exposure, we need sensitive biomarkers to track short- and long-term effects. Circulating small extracellular vesicles (sEVs) (<200 nm) traffic biological molecules throughout the body and may have diagnostic value as biomarkers for disease. The purpose of this study was to identify the microRNA (miRNA) profile in circulating sEVs derived from human plasma following repetitive HI exposure.MethodsHealthy adult (aged 18–35 years) soccer players were randomly assigned to one of 3 groups: the HI group performed 10 standing headers, the leg impact group performed 10 soccer ball trapping maneuvers over 10 min, and the control group did not participate in any soccer drills. Plasma was collected before testing and 24 h afterward, and sEVs were isolated and characterized via nanoparticle tracking analysis. Next-generation sequencing was utilized to identify candidate miRNAs isolated from sEVs, and candidate microRNAs were analyzed via quantitative polymerase chain reaction. In silico target prediction was performed using TargetScan (Version 7.0; targetscan.org) and miRWalk (http://mirwalk.umm.uni-heidelberg.de/) programs, and target validation was performed using luciferase reporter vectors with a miR-7844-5p mimic in human embryonic kidney (HEK) 293T/17 cells.ResultsPlasma sEV concentration and size were not affected across time and group following repetitive HI exposure. After 24 h, the HI read count from next-generation sequencing showed a 4-fold or greater increase in miR-92b-5p, miR-423-5p, and miR-24-3p and a 3-fold or greater decrease in miR-7844-5p, miR-144-5p, miR-221-5p, and miR-22-3p. Analysis of quantitative polymerase chain reaction revealed that leg impact did not alter the candidate miRNA levels. To our knowledge, miR-7844-5p is a previously unknown miRNA. We identified 8 miR-7844-5p mRNA targets: protein phosphatase 1 regulatory inhibitor subunit 1B (PPP1R1B), LIM and senescent cell antigen-like domains 1 (LIMS1), autophagy-related 12 (ATG12), microtubule-associated protein 1 light chain 3 beta (MAP1LC3B), integrin subunit alpha-1 (ITGA1), mitogen-activated protein kinase 1 (MAPK1), glycogen synthase kinase 3β (GSK3β), and mitogen-activated protein kinase 8 (MAPK8).ConclusionCollectively, these data indicate repetitive HI exposure alters plasma sEV miRNA content, but not sEV size or number. Furthermore, for the first time we demonstrate that previously unknown miR-7844-5p targets mRNAs known to be involved in mitochondrial apoptosis, autophagy regulation, mood disorders, and neurodegenerative disease.     

当代自由主义的困境——论哈耶克、约翰·顿、罗尔斯和罗蒂

非常感谢高瑞泉教授的邀请,使我十分荣幸能有机会来华东师范大学主持第九期大夏讲坛。今天我演讲的题目是“当代自由主义的困境——论哈耶克、约翰&;#183;顿、罗尔斯和罗蒂”。正如在座诸位知道的那样,这个演讲其实是我这次在华东师范大学系列演讲的第三讲。前两讲的题目分别是“人类学文化观念中的知识论意蕴”和“文化观念中的知识论意蕴：中西哲学的讨论”,其出发点就是去探讨如何以哲学思辩的方式来会通中西。     

让我们更多地认识城市与城市研究(二)

本文是美国圣地亚哥大学教授詹姆斯·A·克莱普2008年4月在北京城市学院的演讲,演讲共三场。为便于读者全面地理解克莱普教授的演讲内容,全部演讲内容被编译成一篇。本文是根据演讲记录稿整理后,经克莱普教授本人审定英文稿编译。