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871.
The AC electrothermal technique is very promising for biofluid micropumping, due to its ability to pump high conductivity fluids. However, compared to electroosmotic micropumps, a lack of high fluid flow is a disadvantage. In this paper, a novel AC multiple array electrothermal (MAET) micropump, utilizing multiple microelectrode arrays placed on the side-walls of the fluidic channel of the micropump, is introduced. Asymmetric coplanar microelectrodes are placed on all sides of the microfluidic channel, and are actuated in different phases: one, two opposing, two adjacent, three, or all sides at the same time. Micropumps with different combinations of side electrodes and cross sections are numerically investigated in this paper. The effect of the governing parameters with respect to thermal, fluidic, and electrical properties are studied and discussed. To verify the simulations, the AC MAET concept was then fabricated and experimentally tested. The resulted fluid flow achieved by the experiments showed good agreement with the corresponding simulations. The number of side electrode arrays and the actuation patterns were also found to greatly influence the micropump performance. This study shows that the new multiple array electrothermal micropump design can be used in a wide range of applications such as drug delivery and lab-on-a-chip, where high flow rate and high precision micropumping devices for high conductivity fluids are needed. 相似文献
872.
We describe a microfluidic device for on-chip chemical processing, such as staining, and subsequent washing of cells. The paper introduces “separator walls” to increase the on-chip incubation time and to improve the quality of washing. Cells of interest are concentrated into a treatment stream of chemical reagents at the first separator wall for extended on-chip incubation without causing excess contamination at the output due to diffusion of the unreacted treatment chemicals, and then are directed to the washing stream before final collections. The second separator wall further reduces the output contamination from diffusion to the washing stream. With this approach, we demonstrate on-chip leukocyte staining with Rhodamine 6G and washing. The results suggest that other conventional biological and analytical processes could be replaced by the proposed device. 相似文献
873.
H. A. Guidobaldi Y. Jeyaram C. A. Condat M. Oviedo I. Berdakin V. V. Moshchalkov L. C. Giojalas A. V. Silhanek V. I. Marconi 《Biomicrofluidics》2015,9(2)
Many self-propelled microorganisms are attracted to surfaces. This makes their dynamics in restricted geometries very different from that observed in the bulk. Swimming along walls is beneficial for directing and sorting cells, but may be detrimental if homogeneous populations are desired, such as in counting microchambers. In this work, we characterize the motion of human sperm cells ∼60 μm long, strongly confined to ∼25 μm shallow chambers. We investigate the nature of the cell trajectories between the confining surfaces and their accumulation near the borders. Observed cell trajectories are composed of a succession of quasi-circular and quasi-linear segments. This suggests that the cells follow a path of intermittent trappings near the top and bottom surfaces separated by stretches of quasi-free motion in between the two surfaces, as confirmed by depth resolved confocal microscopy studies. We show that the introduction of artificial petal-shaped corrugation in the lateral boundaries removes the tendency of cells to accumulate near the borders, an effect which we hypothesize may be valuable for microfluidic applications in biomedicine. 相似文献
874.
Toby C. Rider 《国际体育史杂志》2015,32(1):37-52
Due to the decision of the Soviet Union and nearly all of its East European satellites to withdraw from the 1984 Los Angeles Summer Games, the communist media provided audiences in the Soviet bloc with few reports on the sporting events in the Olympic city. When Radio Free Europe and Radio Liberty, broadcasters funded by the US government, attempted to fill this ‘information gap’ by beaming coverage of the Los Angeles Games behind the Iron Curtain, the Soviet Union protested to the International Olympic Committee. They claimed, with a mixture of fact and fiction, that the Radios were tools of the American intelligence establishment and accused them of broadcasting ‘subversive’ propaganda to Eastern Europe and the USSR. Stirred into action by this Soviet manoeuvre, leading spokesmen for the Radios were joined by government officials, private citizens, US Olympic Committee members and the American media in a concerted attempt not only to defend the work of the broadcasters, but also to secure their press accreditation for Los Angeles. 相似文献
875.
L. Yu S. M. Grist S. S. Nasseri E. Cheng Y.-C. E. Hwang C. Ni K. C. Cheung 《Biomicrofluidics》2015,9(2)
Creating multicellular tumor spheroids is critical for characterizing anticancer treatments since they may provide a better model of the tumor than conventional monolayer culture. Moreover, tumor cell interaction with the extracellular matrix can determine cell organization and behavior. In this work, a microfluidic system was used to form cell-laden core-shell beads which incorporate elements of the extracellular matrix and support the formation of multicellular spheroids. The bead core (comprising a mixture of alginate, collagen, and reconstituted basement membrane, with gelation by temperature control) and shell (comprising alginate hydrogel, with gelation by ionic crosslinking) were simultaneously formed through flow focusing using a cooled flow path into the microfluidic chip. During droplet gelation, the alginate acts as a fast-gelling shell which aids in preventing droplet coalescence and in maintaining spherical droplet geometry during the slower gelation of the collagen and reconstituted basement membrane components as the beads warm up. After droplet gelation, the encapsulated MCF-7 cells proliferated to form uniform spheroids when the beads contained all three components: alginate, collagen, and reconstituted basement membrane. The dose-dependent response of the MCF-7 cell tumor spheroids to two anticancer drugs, docetaxel and tamoxifen, was compared to conventional monolayer culture. 相似文献
876.
877.
题目:一种支持产品平台设计的多准则模块划分方法 总被引:2,自引:0,他引:2
目的:研究多准则约束下的产品模块划分方法,为企业建立稳健的模块化产品平台奠定基础。方法:采用改进的多目标进化算法对建立的多准则模块划分数学模型求解,并采用模糊集合评价机制进行最优解的寻取,得到基于多准则模块划分方法的产品模块划分结果。结论:通过改进的多目标进化算法求解多准则模块划分模型,能够得到有效支持产品平台设计的产品模块划分方案。通过与已有优化方法的比较验证了本文提出的多准则模块划分方法的优越性。 相似文献
878.
The use of structural equation modeling (SEM) in communication research has become increasingly popular in recent years, with SEM being commonly used as the primary method of data analysis in studies published within the field’s journals. However, despite its widespread use, there are persistent misapplications of SEM in published empirical studies in the communication sciences. Of these types of misuse, the most troubling are those related to the exploitation of flexibilities in data collection and analysis. Given the number of decisions that must be made when analyzing and reporting structural equation models, decisions which often remain undisclosed, the potential for researchers to exploit flexibilities in SEM research is high. To assess the extent of this problem, as well as the more longstanding types of misuse (i.e., omission of information and disclosed “bad practice”) identified by prior studies, we undertake a methodical review of studies employing structural equation modeling within major journals in the field of communication between the years 2007 and 2011. Articles were coded on the characteristics of the sample, characteristics of the model tested, as well as for various potential problems that we defined a priori. The most serious issues are identified, and recommendations for future standards in SEM research are suggested. 相似文献
879.
Maria C. Porcal-Gonzalo 《Journal of Map & Geography Libraries》2015,11(3):289-329
In recent years, there has been a growing recognition of the fact that the proper management of information has to include concern for its preservation and future re-use. This ambition is particularly significant for geospatial data. The primary aim of this article is to define the European and Spanish frameworks for the preservation and reutilization of geographical information, with the ultimate goal of proposing an articulated, lifecycle-based data management model. With this, the intent is to shed some light on a complex, difficult and ever-evolving subject.The article is divided into two main parts. The first part begins with a conceptual justification for geospatial data management and continues by synthesizing the current normative and technological framework for the preservation and reutilization of geographical information. Within this context, the implementation of Spatial Data Infrastructures (SDI) in Spain is evaluated. The second part begins with a case study from Spain that aims to identify different agents and flows that influence the lifecycle of geographical data, as well as the strengths and weaknesses found in each stage of the process under examination. Finally, the foregoing ideas are used in order to propose a SDI-related system for the management of geographical information. 相似文献