Rapid bioparticle concentration and detection by combining a discharge driven vortex with surface enhanced Raman scattering

Rapid concentration and detection of bacteria in integrated chips and microfluidic devices is needed for the advancement of lab-on-a-chip devices because current detection methods require high concentrations of bacteria which render them impractical. We present a new chip-scale rapid bacteria concentration technique combined with surface-enhanced Raman scattering (SERS) to enhance the detection of low bacteria count samples. This concentration technique relies on convection by a long-range converging vortex to concentrate the bacteria into a packed mound of 200 μm in diameter within 15 min. Concentration of bioparticle samples as low as 10⁴ colony forming units (CFU)∕ml are presented using batch volumes as large as 150 μl. Mixtures of silver nanoparticles with Saccharomyces cerevisiae, Escherichia coli F-amp, and Bacillus subtilis produce distinct and noticeably different Raman spectra, illustrating that this technique can be used as a detection and identification tool.     

Medical student attitudes to the autopsy and its utility in medical education: A brief qualitative study at one UK medical school

Attending postmortems enables students to learn anatomy and pathology within a clinical context, provides insights into effects of treatment and introduces the reality that patients die. Rates of clinical autopsies have declined and medical schools have cut obligatory autopsy sessions from their curricula making it difficult to assess medical student perceptions of, and attitudes towards, the educational value of autopsy. Our aim was to investigate these perceptions by designing a brief qualitative study comprising nominal technique and focus group discussions with Cambridge Graduate Course students, all of whom had attended autopsies. Three general themes emerged from the focus group discussions: the value of autopsy as a teaching tool and ways the experience could be improved, the initial impact of the mortuary and the autopsy itself, and the “emerging patient”—an emotional continuum running from cadaver to autopsy subject and living patient. Educational benefits of autopsy‐based teaching included greater understanding of anatomy and physiology, greater appreciation of the role of other health care professionals and an enhanced appreciation of psycho‐social aspects of medical practice. Students suggested improvements for ameliorating the difficult emotional consequences of attendance. We conclude that autopsy‐based teaching represents a low‐cost teaching technique which is highly valued by students and has application to many diverse medical specialties and skills. However, careful preparation and organization of sessions is required to maximize potential educational benefits and reduce any negative emotional impact. Anat Sci Educ 7: 87–96. © 2013 American Association of Anatomists.     

Assessment of total body water and its compartments in elite judo athletes: comparison of bioelectrical impedance spectroscopy with dilution techniques

Bioelectrical impedance spectroscopy (BIS) is an inexpensive and non-invasive technique to measure total body water (TBW), extracellular water (ECW), and intracellular water (ICW). The purpose of this study was to validate TBW, ECW and ICW assessed by BIS, using dilution techniques as the reference method (REF) in elite judo athletes. Thirty-two Portuguese elite male judo athletes were evaluated during a period of weight stability. TBW, ECW and ICW were assessed by BIS (Xitron 4000). Deuterium and bromide dilution techniques were used as the criterion method for measuring TBW and ECW, while ICW was calculated as the difference between the two. BIS explained 96%, 77% and 94% of the total variability from REFs for TBW, ECW and ICW, respectively. BIS also demonstrated high precision (ρ ≥ 0.88) and accuracy (C_b = 0.98), with a minimum concordance coefficient correlation of 0.87 for ECW. The mean bias demonstrated that BIS slightly overestimated the REF in 1.1 kg (2.3%), 0.3 kg (1.6%) and 0.8 kg (2.7%) for TBW, ECW and ICW, respectively. The 95% limits of agreement ranged from ?1.2 to 3.3 kg in TBW, from ?1.8 to 2.4 kg in ECW and from ?1.0 to 2.6 kg in ICW. A non-significant trend was found between the difference and the mean of reference and alternative methods. These findings highlight the efficacy of BIS as a valid non-biased tool for the assessment of TBW and its compartments in elite male judo athletes, during a period of weight stability.     

Forget EPSSs: How You Can Marshal Mobile Geographic Information Systems (GISs) and Spatial Awareness for Performance Improvement

Mobile geolocation applications identify global coordinates of mobile devices and can be enriched with performance‐context data. This technology has potential to integrate job aids, learning bursts, and parts of manuals with work tools (such as checklists) at the point of performance to better support individual and team performance. Emerging uses of mobile geolocation allow human performance technologists to move from generic electronic performance support systems (EPSSs) to spatial support systems. These increase the potential for performers to access more context‐aware information at the time and space in which the actions occur. Mobile geolocation can also automatically send data about the performance to enterprise databases so that actions taken and the use of training or job aids at the point of performance can be tracked. This article explores how mobile geolocation functionality can be orchestrated to support performance, enrich enterprise databases, and aid human performance technologists.     

Student attitudes toward cadaveric dissection at a UK medical school

A more humanistic approach toward dissection has emerged. However, student attitudes toward this approach are unknown and the influences on such attitudes are little understood. One hundred and fifty-six first-year medical students participated in a study examining firstly, attitudes toward the process of dissection and the personhood of the cadaver and secondly, the extent to which gender, anxiety, exposure to dissection, bereavement and prior experience of a dead body influenced these attitudes. Attitudes toward dissection were assessed by of levels of agreement toward eleven statements and by selection of adjectives describing possible feelings toward dissection. Students were asked about recent bereavement, whether they had seen a dead body prior to starting their course and exposure to dissection when completing the questionnaire. Validated instruments were used to measure disposition toward generalized anxiety (Hospital Anxiety and Depression Scale) and toward death anxiety (Collett-Lester Death Anxiety Scale). Between 60% and 94% of students held positive attitudes toward the process of dissection and over 70% of students selected 2 or fewer negative adjectives. Students' attitudes toward the personhood of the cadaver were more disparate. Disposition toward anxiety (particularly death anxiety), and exposure to dissection, influenced attitudes. Female gender and recent bereavement exerted a negative influence. Students with higher levels of anxiety experienced more negative feelings and those recently bereaved were less enthusiastic about dissection. Anticipation of dissection may be worse than reality. Sensitive preparation of students prior to entering the dissecting room for the first time may be beneficial.     

RELIABILITY AND STRUCTURAL VALIDITY OF THE TEACHER RATING SCALES OF EARLY ACADEMIC COMPETENCE

Currently, there are few strengths‐based preschool rating scales that sample a wide array of behaviors believed to be essential for early academic success. The purpose of this study was to assess the factor structure of a new measure of early academic competence for at‐risk preschool populations. The Teacher Rating Scales of Early Academic Competence (TRS‐EAC) includes two broad scales (Early Academic Skills and Early Academic Enablers) and was completed by 60 teachers for 440 children enrolled in Head Start and public preschool classrooms. Evidence from two exploratory factor analyses supported a five‐factor solution for the Early Academic Skills Scale (Creative Thinking, Critical Thinking Skills, Numeracy, Early Literacy, and Comprehension) and a five‐factor solution for the Early Academic Enablers Scale (Approaches to Learning, Social and Emotional Competence, Fine Motor Skills, Gross Motor Skills, and Communication). TRS‐EAC scores also demonstrated good to excellent reliability and were related to children's performance on direct measures of early academic skills.     

A roadmap for forming successful interdisciplinary education research collaborations: a reflective approach

Current literature about interdisciplinary education research is focused on three points: conceptual definitions of interdisciplinarity, the need for interdisciplinary research to tackle the advent of problem-based research and the positive curriculum outcomes to be gained from interdisciplinary research. While this research is important, it does not always include an account of the often complex and politicised interactions that might affect the outcomes of interdisciplinary research groups. This paper provides one possible ‘roadmap’ for successful interdisciplinary collaboration. It is based on a reflective case study of the authors’ own formation of an interdisciplinary research group and the practical resolutions to both the theoretical and the practical issues involved in achieving interdisciplinarity in education research.     

Puppet play as interactive approach in drug abuse prevention

The national strategies of drug abuse prevention across Europe have come to recognise that the drugs abuse problem presents a complex set of issues of which there is no simple solution. There is a considerable increase in investment in prevention, treatment and harm-reduction activities and increased focus on supply reduction. School settings are the focus of most attempts to implement effective prevention programmes. Prevention in schools does not only focus on drugs alone, but also includes personal and social skills, often with family involvement and involvement of local community. The school is an ideal setting for drug abuse prevention and development of active and responsible attitudes against drug abuse. Successful school drug-prevention programmes include personal skills training （decision-making, coping and goal-setting）, social skills training （assertiveness, resisting peer-pressure）, drug education （knowledge about drugs and the consequences of taking them） and developing attitudes （especially correcting misconceptions about peer group drug use）. Effective drug education programmes incorporate a range of activities which provide students with relevant factual information, the opportunity to consider their attitudes and values, and the values of others. For effective delivery of prevention programmes, interactive teaching is better than didactic teaching alone. In that sense, the puppet play could be used as an interactive technique. Puppets help the students engage actively in communicating their emotions, opinions and experiences, and learn about the drug abuse problem. By the means of puppetry and dramatic play, the students may learn about drugs and the consequences of taking them.     

Fly Me to the Moon

这首歌原曲本是一首华尔兹舞曲,叫做（《In Other Words》,1954年由BartHoward创作？1962年经Joe Hannel改编后大受欢迎？而爵士女歌手Diana Krall随性的现场演唱版．则给这首被翻唱和重新演绎了无数次的歌曲,赋予了新的活力。