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931.
K. Pratibha Usha Anand Rajni Agarwal 《Indian journal of clinical biochemistry : IJCB》2004,19(2):128-131
Serum adenosine deaminase (ADA), 5′ nucleotidase (5′NT) and malondialdehyde (MDA) were estimated in patients with acute infective
hepatitis (AIH) along with the routine parameters of liver disease. Present study is done to evaluate these special parameters
in patients with clinical history of AIH and to assess the utility of these parameters as diagnostic/ prognostic indices of
liver function and to correlate special parameters with routine live function tests (LFT). ADA, 5′NT and MDA along with routine
LFT was estimated in 25 patients with AIH and 25 samples from healthy voluntary blood donars served as the control group.
Routine LFT was estimated by standard clinical chemistry procedures on dade behring analyser and ADA, 5′NT and MDA were estimated
by berthlot reaction, fiske and subbarao method and thiobarbituric acid method respectively.
Statistical analysis showed that serum ADA, 5′NT and MDA were significantly higher in patients as compared with the controls.
There was a significant positive correlation between ADA and total bilirubin and MDA and total bilirubin. Hence we can conclude
that these tests would be more sensitive to diagnose the patients with AIH and that the raised bilirubin levels could be looked
upon, as a protective mechanism which the liver has evolved in order to combat oxidative stress. 相似文献
932.
933.
The objective of this study was to evaluate acrosin activity in spermatozoa of infertile Nigerian men and to compare with
those of the fertile men. The acrosin activity was evaluated using the Acroscreen reagent kit. The acrosin activity of the
fertile men was 40.7±5.2 mIU/106 sperm (range 30.1–51.3) and those of the infertile men was 22.4±8.33 mIU/106 sperm (range 5.7–39.1). The difference in the mean was statistically significant (P<0.001). There was a subpopulation of
the infertile men who had their acrosin activity within normal range of the fertile men, 32.9±1.57 mIU/106 sperm. But the difference in the mean was statistically significant (P<0.001). Acrosin activity decreased with increased
morphological changes in the spermatozoa. It is concluded that acrosin activity in the infertile Nigerian men is significantly
lower than that in the fertile men. Acrosin activity may also be affected by morphological changes in the spermatozoa. 相似文献
934.
935.
G. M. Sankolli M. I. Khatkhatay M. P. Desai D. K. Pardhe P. C. Ishrad U. M. Joshi 《Indian journal of clinical biochemistry : IJCB》1992,7(1):71-74
Aprototype kit for the detection of occurrence of ovulation based on urinary pregnanediol glucuronide estimations by ELISA
was developed in our laboratory. This kit was sent for evaluation at 4 centres in Bombay and Pune, India. The diagnosis of
ovulation/anovulation based on the results of the kit is comparable with that based on reference parameters commonly used
(ultrasonography of ovarian follicles and serum progesterone estimations). The accuracy of the test was 93.5%, specificity
was 87.5% and sensitivity was 95.7%. This rapid indigenous test can be very useful in assessing ovulatory status of the woman
by the clinicians in their offices. 相似文献
936.
H.C. Reddy C.P. Reddy M.N.S. Swamy V. Ramachandran 《Journal of The Franklin Institute》1982,313(4):197-206
In this paper a method for the realization of RC transfer immittance functions with an unsymmetrical lattice is presented. The method yields minimal capacitor realizations for Z12(s) with no pole at s = 0 and ?y12(s) having no pole at s = ∞. The number of resistors needed are only two more than the minimum needed. 相似文献
937.
Induction of diabetes by Streptozotocin in rats 总被引:1,自引:0,他引:1
A. Akbarzadeh D. Norouzian M. R. Mehrabi Sh. Jamshidi A. Farhangi A. Allah Verdi S. M. A. Mofidian B. Lame Rad 《Indian journal of clinical biochemistry : IJCB》2007,22(2):60-64
The objective of this study is to induce experimental diabetes mellitus by Streptozotocin in normal adult Wistar rats via
comparison of changes in body weight, consumption of food and water, volume of urine and levels of glucose, insulin and C-peptide
in serum, between normal and diabetic rats. Intra-venous injection of 60mg/kg dose of Streptozotocin in adult wistar rats,
makes pancreas swell and at last causes degeneration in Langerhans islet beta cells and induces experimental diabetes mellitus
in the 2–4 days. Induction of experimental diabetes mellitus is indeed the first step in the plan of purification of pancreatic
Langerhans islet cells of normal rats for transplanting under the testis subcutaneous of experimentally induced diabetic rats.
Streptozotocin induces one type of diabetes which is similar to diabetes mellitus with non-ketosis hyperglycemia in some animal
species. For induction of experimental diabetes in male adult rats weighted 250–300 grams (75–90 days), 60mg/kg of Streptozotocin
was injected intravenously. Three days after degeneration of beta cells, diabetes was induced in all animals. The diabetic
and normal animals were kept in the metabolic cages separately and their body weight, consumption of food and water, urine
volume, the levels of serum glucose, insulin and C-peptide quantities in all animals were measured and then these quantities
were compared. For a microscopic study of degeneration of Langerhans islet beta cells of diabetic rats, sampling from pancreas
tissue of diabetic and normal rats, staining and comparison between them, were done. Induction of diabetes with Streptozotocin
decreases Nicotinamide-adenine dinucleotide (NAD) in pancreas islet beta cells and causes histopathological effects in beta
cells which probably intermediates induction of diabetes. In this study, we used Streptozotocin for our experiments in induction
of experimental diabetes mellitus. After Induction of diabetes, consumption of food and water, volume of urine and glucose
increased in the diabetic animals in comparison with normal animals, but the weight of body and the volume of insulin and
C-peptide decreased in the diabetic animals. Sampling and staining of pancreas tissue of diabetic and normal rats showed that
the Langerhans islet beta cells of diabetic rats have been clearly degenerated. In three days, Streptozotocin makes pancreas
swell and at last causes degeneration in Langerhans islet beta cells and induces experimental diabetes. It also changes normal
metabolism in diabetic rats in comparison with normal rats. Consumption of water and food, volume of urine, serum glucose
increases in diabetic animals in comparison with normal rats but the levels of serum insulin, C-peptide and body weight decreases. 相似文献
938.
This article addresses file maintenance of subject headings in the National Library of Medicine's MEDLINE, a bibliographic retrieval file which is indexed using Medical Subject Headings (MeSH), NLM's subject authority. The emphasis is class maintenance in which sets of records in MEDLINE are changed by maintenance actions which reflect yearly changes in MeSH. Specific types of maintenance action are described with examples to highlight problem areas. Class maintenance failures, checking class maintenance, and informing end-users of MeSH changes resulting in class maintenance are discussed. Technical constraints and feasibility are not analyzed in detail; rather, the approach is geared toward requirements and impact of maintenance from the standpoint of vocabulary specialists and end-users. The article concludes with a brief statement on defining file maintenance policy. 相似文献
939.
The rate of citation duplication was examined in three databases: MEDLINE, BIOSIS, and LIFE SCIENCES COLLECTION. Duplicate citations were found to be more pertinent than unique citations. The duplicate citations came from a highly compact literature, while those from a single database were very widely scattered. The pertinent duplicated citations were more likely to be retrieved in searches that had more terms overall, had a higher percentage of thesaurus terms, and had terms which appeared in both title and abstract. These results suggest that the rate of duplication of citations in multidatabase searches may be used to rank output according to probable pertinence. 相似文献
940.
Proteins and glutathione (GSH) levels were estimated in the experimental groups. Levels of both proteins and glutathione in
the control and the treated groups were found to be significantly decreased compared to the normal group. The results show
that supplementation of riboflavin has beneficial effect by alleviating the oxidant injuries to GSH and proteins, which are
involved in the maintenance of transparency and protection of thiol groups of the lens. Thus, riboflavin has an active role
in the lens metabolism and has a potential to improve the metabolic activity of the cataractous lenses. 相似文献