Genetic analysis of fruit shape traits at different maturation stages in sponge gourd

The fruit shape is important quantitative trait closely related to the fruit quality. However, the genetic model of fruit shapes has not been proposed. Therefore, in the present study, analysis of genetic effects for fruit shape traits （fruit length and fruit perimeter） in sponge gourd was conducted by employing a developmental genetic model including fruit direct effects and maternal effects. Analysis approaches of unconditional and conditional variances were applied to evaluate the genetic behavior of fruit shape traits at economical and physiological maturation times. The results of variance analysis indicated that fruit length and fruit perimeter were simultaneously affected by fruit direct genetic effects and maternal effects. Fruit direct genetic effects were relatively more important for fruit shape traits at whole developmental period. The gene expression was most active at the economical maturation stage （1-12 d after flowering） for two shape traits, and the activation of gene was mostly due to direct dominance effects at physiological maturation stage （13-60 datter flowering）. The coefficients due to different genetic effects, as well as the phenotypic correlation coefficients, varied significantly between fruit shape traits themselves at various maturation stages. The results showed that it was relatively easy to improve fruit shape traits for industrial purpose by carefully selecting the parents at economical maturation stage instead of that at physiological maturation stage.     

A rapid and accurate 3-（4,5-dimethyl thiazol-2-yl）-2,5-diphenyl tetrazolium bromide colorimetric assay for quantification of bacteriocins with nisin as an example

The objective of this study is to propose a more accurate and faster MTT 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide colorimetric assay (MCA) for quantitative measurement of polypeptide bacteriocins in solutions with nisin as an example. After an initial incubation of nisin and indicator bacterium Micrococcus luteus NCIB 8166 in tubes, MTT was added for another incubation period. After that, nisin was quantified by estimating the number of viable bacteria based on measuring the amount of purple formazan produced by cleavage of yellow tetrazolium salt MTT. Then MCA was compared to a standard agar diffusion assay (ADA). The results suggested a high correlation coefficient (r2=0.975±0.004) between optical density (OD) and the inhibitory effect of nisin on a bacterial strain Micrococcus luteus NCIB 8166 at a range of 0.125～32 IU/ml. The MCA described in this study was very quick. Quantification of nisin took only 7～8 h and the detection limit was at the level of 0.125 IU/ml when compared to 12 IU/ml and 24～28 h for ADA. The MCA provides an accurate and rapid method for quantifi-cation of nisin in solutions and is expected to be used for quantification of other antimicrobial substances.     

机电控制器类课程虚拟实验系统的研究与开发

针对机电控制器类课程传统实验平台的缺点,提出了一种有效的解决方案:开发建立相应课程的虚拟实验系统.分别介绍了采用Proteus软件开发单片机课程虚拟实验系统和采用组态王软件开发PLC虚拟实验系统的研发思路、内容及实现方法,阐述了运用机电控制器类课程虚拟实验系统的教学效果及推广意义.     

纳米碳掺杂TiO2的制备、表征及可见光光催化性能实验

结合科研成果,开设了一种简便的纳米碳掺杂TiO2的制备、表征和可见光光催化性能的实验。实验内容涵盖材料化学、无机合成、仪器分析、催化等专业知识,涉及材料和环境等领域。可作为大学化学综合型实验,也可以此为基础升格为大学化学综合创新型实验。     

技术基础课实践教学模式改革研究与实践

以“实践教学是高职教育主体教学”的理念,架构技术基础课实践教学的“4-3-1模式”,使学生的实践技能得了到更好的训练和提升.在改革实践中探索了真正实现高职培养目标的技术基础课实践教学新模式.     

实验教学质量监控体系的研究与探索

实验教学作为本科教学的重要组成部分,与理论教学相比,实验教学的质量监控和管理有很多不同之处.从实验教学质量监控体系建立的目的和原则进行讨论,根据农科类高校实践教学的实际情况,制定了实验教学质量监控体系,并进一步提出了具体措施.     

全国职业院校技能大赛对职业教育理念和观念的影响

职业教育理念包括育人为本理念、终身化理念、市场导向理念、校企合作理念和注重素质理念等;职业教育观念包括发展观念、价值观念、形象观念、改革创新观念和环境观念等。通过对职业院校教师、学生及学生家长的调查发现,全国职业院校技能大赛提升了职业教育的社会影响力,推动了职业院校办学理念的创新,促进了尊重技能、重视职业教育风气的形成,引领了职业教育教学改革。     

高校非英语专业学生英语语音水平与语言水平的相关研究

通过对31名非英语专业学生的英语语音水平和语言水平的相关性进行分析发现,非英语专业学生的语音测试和四级考试成绩均未达到理想水平,语音测试中连读、失去爆破和不完全爆破尤为薄弱;非英语专业学生的语音水平与语言水平呈显著相关,尤其表现在单词发音、语句重音、连读、意群停顿、元音音素、辅音音素和失去爆破等方面;非英语专业学生在英语语音水平与语言水平的相关性上存在性别差异。     

一种高压条件下测定吸附剂静态吸附容量的装置

采用静态容量法研制开发了适于高压条件下的静态吸附仪,阐述了仪器的工作原理和功能结构,并采用该仪器测定了活性炭对CO2、CH4和N2的吸附量,获得了吸附剂在不同高压条件下对各气体的吸附容量数据。该吸附仪能直观地分析吸附剂对气体的吸附特性,对吸附剂的工程应用有着重要的指导作用。此外,该仪器能够与上位计算机通过串口进行通讯,实现测量数据的实时显示与存储。