Cloning, expression, purification, and characterization of LC-1 ScFv with GFP tag

INTRODUCTION Because of its low molecular weight and ability to fluoresce independently (George, 1997), the new molecular tag, green fluorescent protein (GFP), has become more and more popular after Prasher et al.(1992) cloned its cDNA in 1992. There are many reports describing the co-expression of GFP and a specific antibody or cytokine gene, with the fusion protein expressing the fluorescent activity and bio-logical activity of the complement protein (Haraguchi et al., 1999; Mclean…     

发挥电大优势为社区教育提供社会化服务

社区教育是终身教育的一部分。在我国构筑终身教育体系，创建学习化社会，应立足于社区。电大以自己的系统优势、设施优势和教育资源优势为社区教育提供全方位的支持服务，完全有能力参与和推动社区教育。在实现教育的大众化普及化，促进全民终身学习的同时电大又可以得到自身的发展。     

传统思维与中国古代建筑

本文主要从思维方式的角度，研究传统思维与中国古代建筑的关系，阐述中国古代建筑中所表现的整体意识、和合及实用理性精神。     

试论我国高校创新体系内涵

本文从高等学校创新体系这一概念的提出入手,较为系统地对当前我国高教理论界颇具代表性的高等学校创新体系的表述进行了辨析,在此基础上,提出了新的表述并对其内涵进行了全新的阐述.     

试论大学生思想政治教育方法的创新

新形势下大学生思想政治教育方法的创新必须做到“四个结合”，即政治教育与成才教育相结合，理性教育与感性教育相结合，主导教育与自我教育相结合，解决思想问题与解决实际问题相结合，并采取灵活多样的工作方法。     

试论王树楠对新疆货币思想的贡献

本文试图从对历史文献的解读中，探讨王树楠对新疆货币思想的贡献，从而加深对新疆近代货币思想变迁的认识，继承和发扬新疆优秀历史文化遗产，为当前的经济建设作一有益的借鉴。     

面向21世纪的社会科学课程

21世纪应该是个人文教育空前发展的时代 ,社会科学也越来越凸现了它的重要性。文章通过比较世界各国的课程教学情况 ,在课程内容方面论述了 2 1世纪的社会科学课程。     

论李煜前期词与南唐亡国之关系

李煜的前期词与其性格是统一的,它们都受唐五代词风的影响,同时又是南唐社会的一个缩影,这些因素综合在一起,决定了南唐必然亡国的命运。本文就是从这个角度讨论了李煜前期词与其亡国的关系。     

Tandem Biocatalysis by CotA-TJ102@UIO-66-NH2 and Novozym 435 for Highly Selective Transformation of HMF into FDCA

2,5-Furandicarboxylic acid(FDCA) is a potential biorenewable chemical for applications including plastics, polyamides, drugs, etc. The selective biosynthesis of FDCA from 5-hydroxymethylfurfural(HMF) by a specific enzyme poses a great challenge. In this study, we reported an efficient strategy to produce FDCA from HMF by the tandem biocatalysis of laccase(Cot A-TJ102@UIO-66-NH_2) and Novozym 435. For the first step, a nanoparticle metal–organic framework was synthesized as a carrier to immobilize Cot A-TJ102@UIO-66-NH_2, which was assigned for the production of 5-formyl-2-furancarboxylic acid(FFCA) and featured an enzyme loading of 255.54 mg/g, specific activity of 135.90 U/mg, and solid loading ratio of 99.65%. Under optimal conditions, an ideal FFCA yield of 98.5% was achieved, and the Cot A-TJ102@UIO-66-NH_2 presented a high recycling capacity after 10 cycles. For the second step, Novozym 435 was applied for the further conversion of FFCA into FDCA, presenting a high FDCA yield of 95.5% under the optimized conditions. Novozym 435 also exhibited a high recyclability after eight cycles. As a result, the tandem biocatalysis strategy provided a 94.2% FDCA yield from HMF, indicating its excellence as a method for FDCA production.     

洋务教育改革与明治维新教育改革之比较

本文对洋务教育与明治维新教育在改革的目的、改革的指导思想、改革所形成的体制三个方面进行比较研究，以探究中日两国改革结果不同的原因。这对我国当今教育改革具有借鉴意义。