排序方式: 共有65条查询结果,搜索用时 15 毫秒
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Eriksen J Thilsted AH Marie R Lüscher CJ Nielsen LB Svendsen WE Szabo P Kristensen A 《Biomicrofluidics》2011,5(3):31101-311014
A method of in situ chromosome immobilisation and DNA extraction in a microfluidic polymer chip was presented. Light-induced local heating was used to induce poly(N-isopropylacrylamide) phase transition in order to create a hydrogel and embed a single chromosome such that it was immobilised. This was achieved with the use of a near-infrared laser focused on an absorption layer integrated in the polymer chip in close proximity to the microchannel. It was possible to proceed to DNA extraction while holding on the chromosome at an arbitrary location by introducing protease K into the microchannel. 相似文献
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Vibha Uppal Chittranjan Vij Gurdeep Kaur Bedi Anil Vij Basu Dev Banerjee 《Indian journal of clinical biochemistry : IJCB》2013,28(4):336-341
The study was planned to assess the prevalence of thyroid disorders in type 2 diabetes in North Indian population and to correlate the serum insulin and glycosylated haemoglobin levels with thyroid hormones. It is a case control study. One hundred and twenty patients of type 2 diabetes were included in the study along with 117 adults of the same age group and normal glucose levels as controls. All blood samples were taken from subjects who fasted for at least 12 h before the blood collection. Glycosylated hemoglobin was determined by ion exchange chromatography and serum insulin and thyroid hormones were assessed through enzyme linked immunosorbent assay. Fasting blood glucose and glycosylated haemoglobin levels were significantly higher in diabetics showing a poor glucose control. Serum tri-iodothyronine values were significantly lower in diabetics. There was a significant correlation between glycosylated haemoglobin and thyroid hormones. There was no correlation between serum insulin and thyroid hormones. 相似文献
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Assessment is becoming important from many different perspectives. Universities are planning, developing, and testing various assessment models to satisfy stakeholders. Many accrediting agencies are also requiring assessment plans and their implementation from universities for reaccreditation. This article describes how assessment was used to continuously improve a database course in a Web MBA program, and combines Deming's Plan‐Do‐Check‐Act (PDCA) cycle with Bloom's taxonomy to develop rubrics for problem solving learning objectives in an online course. Results from direct and indirect measurements are used to improve the course. Specifically, two semester results are compared for course assessment. Results show some improvement; however, the course requires continuous improvement to meet benchmarks. The model presented here is generic in nature and should be useful for all educators who plan to initiate or continue the assessment and continuous improvement process. Instead of reinventing the wheel, educators can learn from our experiences and use the results as a starting point for their own assessment programs. 相似文献
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Mangala Anil Hirwade 《International Information and Library Review》2013,45(3):154-163
Providing government information, services, products and transactions electronically has the potential benefit of accessibility for a wider audience, political and administrative transparency, and improved service delivery. By using e-government websites, citizens can conveniently access government information and services and gain greater opportunities to participate in democratic processes. The present study aims to evaluate National Portal of India, which provides single window access to 601 e-government portals and websites in India. A total of 1576 online services are provided by these portals responding to information needs of the citizens. Ranking of the state and union territory portals has been done based on the number of online services they provide. The paper also focuses on the digitization of documents, acts, rules and schemes of central and state government departments and their availability and accessibility through government portals. 相似文献
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Mangala Anil Hirwade Ujwala Anil Nawlakhe 《International Information and Library Review》2013,45(1):28-39
‘Postage stamps’, basically as a token for payment of postal taxes, are now being recognized as an information source and historical document in recording national achievements that visually convey four core elements: denomination, country name, graphical design, and the textual element. They are a valuable means of communication easily available anywhere to anybody. Realizing them as source of extra revenue, commemorative stamps are made available over time. The information technology (IT) wave changed the hobby of stamp collection to Digital Philately, while information and communication technology (ICT) developments accelerated the hobby further to Virtual Philately.This paper focuses on the aspects of digital and virtual philately. This study aims to evaluate The World Association for the Development of Philately (WADP) Numbering System (WNS) database, which provides free access to authentic postage stamps issued by Universal Postal Union (UPU) member countries and territories. A total of 48,159 stamps with 48 themes were found registered by 184 countries at the time of study. Indian stamps totaled 554 entries with the highest number of entries, 1258, from France, and followed by 1006 from Japan. A ranking was done using themes whereas “Fauna” was observed to be the most popular theme at 14.1% or 6800 stamps under study. For Indian stamps, politics and government, architecture and literature, press and comics are the most prevalent themes with 14.3, 9.8 and 9.8% respectively of the national contribution. The paper also presents a detailed analysis of the 15 Indian stamps commemorating the 14 Indian traditional universities issued over the 50 years of India’s independence. 相似文献
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The South African education system has witnessed significant changes since 1994 when the democratically elected government began the process of dismantling the inherited apartheid order. The primary focus of the transformation process was to address the twin imperative of equity and quality in education, particularly for the historically marginalised black population. A key aspect of this transformation process remains the development of alternative assessment policies. This paper reviews the changes focusing on the stated rationale as well as their underlying assumptions and implications for practice. It argues that the changes have in many respects addressed the most obvious effects of the previous apartheid systems. However, it notes that, notwithstanding the policy intentions, assessment policy since 1994 has favoured a measurement focused-approach in the classroom, which has hindered a shift towards an assessment for learning approach. This, it argues, is partly fuelled by the abiding belief in and commitment to classroom testing and examinations as well as external national assessments as the key criterion for reforming learning and teaching practices in the classroom. 相似文献
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Anil Lachke 《Resonance》2002,7(5):50-58
In the biosphere we find cellulose and hemicellulose as the major polysaccharides. On acid or enzymatic hydrolysis,D-glucose is produced from cellulose andD-xylose is produced from xylans as the major sugar in the hydrolysate. Initially it was believed that yeasts do not fermentD-xylose to ethanol although many are capable of producing xylitol. Twenty years ago, a few yeasts that could convertD-xylose to ethanol were found. Ethanol is viewed as a potential fuel that is available from biomass and hence new methods
to generate ethanol from hitherto inaccessible sources are gaining importance. Biotechnology for efficient utilization of
lignocellulose wastes as fuels relies on the utilization of both the cellulosic as well as hemicellulosic portions of the
biomass. In this article, conversion of xylose into ethanol is discussed. 相似文献