Skeletal muscle glycogen concentration and metabolic responses following a high glycaemic carbohydrate breakfast

The purpose of this study was to examine the influence of a carbohydrate-rich meal on post-prandial metabolic responses and skeletal muscle glycogen concentration. After an overnight fast, eight male recreational/club endurance runners ingested a carbohydrate (CHO) meal (2.5 g CHO?·?kg^?1 body mass) and biopsies were obtained from the vastus lateralis muscle before and 3 h after the meal. Ingestion of the meal resulted in a 10.6?±?2.5% (P?<?0.05) increase in muscle glycogen concentration (pre-meal vs post-meal: 314.0?±?33.9 vs 347.3?±?31.3 mmol?·?kg^?1 dry weight). Three hours after ingestion, mean serum insulin concentrations had not returned to pre-feeding values (0 min vs 180 min: 45?±?4 vs 143?±?21 pmol?·?l^?1). On a separate occasion, six similar individuals ingested the meal or fasted for a further 3 h during which time expired air samples were collected to estimate the amount of carbohydrate oxidized over the 3 h post-prandial period. It was estimated that about 20% of the carbohydrate consumed was converted into muscle glycogen, and about 12 % was oxidized. We conclude that a meal providing 2.5 g CHO?·?kg^?1 body mass can increase muscle glycogen stores 3 h after ingestion. However, an estimated 67% of the carbohydrate ingested was unaccounted for and this may have been stored as liver glycogen and/or still be in the gastrointestinal tract.     

A faster running speed is associated with a greater body weight loss in 100-km ultra-marathoners

Abstract

In 219 recreational male runners, we investigated changes in body mass, total body water, haematocrit, plasma sodium concentration ([Na⁺]), and urine specific gravity as well as fluid intake during a 100-km ultra-marathon. The athletes lost 1.9 kg (s = 1.4) of body mass, equal to 2.5% (s = 1.8) of body mass (P < 0.001), 0.7 kg (s = 1.0) of predicted skeletal muscle mass (P < 0.001), 0.2 kg (s = 1.3) of predicted fat mass (P < 0.05), and 0.9 L (s = 1.6) of predicted total body water (P < 0.001). Haematocrit decreased (P < 0.001), urine specific gravity (P < 0.001), plasma volume (P < 0.05), and plasma [Na⁺] (P < 0.05) all increased. Change in body mass was related to running speed (r = ?0.16, P < 0.05), change in plasma volume was associated with change in plasma [Na⁺] (r = ?0.28, P < 0.0001), and change in body mass was related to both change in plasma [Na⁺] (r = ?0.36) and change in plasma volume (r = 0.31) (P < 0.0001). The athletes consumed 0.65 L (s = 0.27) fluid per hour. Fluid intake was related to both running speed (r = 0.42, P < 0.0001) and change in body mass (r = 0.23, P = 0.0006), but not post-race plasma [Na⁺] or change in plasma [Na⁺] (P > 0.05). In conclusion, faster runners lost more body mass, runners lost more body mass when they drank less fluid, and faster runners drank more fluid than slower runners.     

Influence of ingesting a carbohydrate‐electrolyte solution on endurance capacity during intermittent,high‐intensity shuttle running

The aim of this study was to examine the effects of ingesting a carbohydrate‐electrolyte solution on endurance capacity during a prolonged intermittent, high‐intensity shuttle running test (PIHSRT). Nine trained male games players performed two exercise trials, 7 days apart. On each occasion, they completed 75 min exercise, comprising of five 15‐min periods of intermittent running, consisting of sprinting, interspersed with periods of jogging and walking (Part A), followed by intermittent running to fatigue (Part B). The subjects were randomly allocated either a 6.9% carbohydrate‐electrolyte solution (CHO) or a non‐carbohydrate placebo (CON) immediately prior to exercise (5 ml kg^‐1 body mass) and every 15 min thereafter (2 ml kg^‐1 body mass). Venous blood samples were obtained at rest, during and after each PIHSRT for the determination of glucose, lactate, plasma free fatty acid, glycerol, ammonia, and serum insulin and electrolyte concentrations. During Part B, the subjects were able to continue running longer when fed CHO (CHO = 8.9 ± 1.5 min vs CON = 6.7 ± 1.0 min; P < 0.05) (mean ± s.e.m.). These results show that drinking a carbohydrate‐electrolyte solution improves endurance running capacity during prolonged intermittent exercise.     

Does sensorimotor training improve the static balance of young volleyball players?

The aim of this investigation is to assess the effectiveness of a 6-week balance training (BT) protocol, integrated in regular training sessions, on postural sway of young female volleyball players (n = 26, age 13.0 ± 0.2 years) divided into two groups (intervention and control; 13 per group). Trials were performed for bipedal and unipedal stance conditions before and after the BT protocol, using a pressure platform to collect center-of-pressure (COP) time series that were processed to calculate sway area, COP path length, and maximum displacement range in anteroposterior and mediolateral directions. The intervention group exhibited smaller sway areas in eyes closed conditions (intervention = 42.76 mm², control = 67.60 mm²; p < 0.05) and Romberg quotients (intervention = 1.11 mm, control = 1.82 mm) in bipedal stance, while all the other parameters were unaffected. BT also reduced sway area (intervention = 122.70 mm², control = 187.18 mm²) and anteroposterior COP displacements (intervention = 20.18 mm, control = 22.38 mm) of the non-dominant limb for single-leg stance. No significant change was found for the dominant limb. Although it is possible to hypothesize a beneficial effect of BT on young athletes, further investigations are required to clarify its actual effect on balance performance with respect to normal volleyball training.     

Serological markers of inflammatory bowel disease

Inflammatory bowel disease (IBD) is a heterogeneous group of chronic inflammatory disorders of the gastrointestinal tract with two main distinguishable entities, Crohn’s disease (CD) and ulcerative colitis (UC). IBD-unclassified (IBD-U) is a diagnosis that covers the “grey” zone of diagnostic uncertainty between UC and CD. Current diagnosis of IBD relies on the clinical, endoscopic, radiological, histological and biochemical features, but this approach has shortcomings especially in cases of overlapping symptoms of CD and UC. The need for a diagnostic tool that would improve the conventional methods in IBD diagnosis directed the search towards potential immunological markers, since an aberrant immune response against microbial or endogenous antigens in a genetically susceptible host seems to be implicated in IBD pathogenesis. The spectrum of antibodies to different microbial antigens and autoantibodies associated with IBD is rapidly expanding. Most of these antibodies are associated with CD like anti-glycan antibodies: anti-Saccharomices cerevisiae (ASCA) and the recently described anti-laminaribioside (ALCA), anti-chitobioside (ACCA), anti-mannobioside (AMCA), anti-laminarin (anti-L) and anti-chitin (anti-C) antibodies; in addition to other antibodies that target microbial antigens: anti-outer membrane porin C (anti-OmpC), anti-Cbir1 flagellin and anti-I2 antibody. Also, autoantibodies targeting the exocrine pancreas (PAB) were shown to be highly specific for CD. In contrast, UC has been associated with anti-neutrophil cytoplasmic autoantibodies (pANCA) and antibodies against goblet cells (GAB). Current evidence suggests that serologic panels of multiple antibodies are useful in differential diagnosis of CD versus UC and can be a valuable aid in stratifying patients according to disease phenotype and risk of complications.     

The responses of fatty acid-binding protein and creatine kinase to acute and chronic exercise in junior rowers

The responses of fatty acid-binding protein (FABP) and creatine kinase (CK) were compared in a group of junior rowers during 5 weeks of training. Acute exercise induced a larger increase in FABP (70-362%) than in CK (24-156%). When the chronic effect of exercise was studied, the baseline FABP levels were independent of previous training. However, the baseline CK level was dependent on the training that took place during 1 day before (F = 4.362, p < .01) and on the combined training on the previous 2 days (F = 4.606, p < .005). The present results suggest that FABP and CK could be used to monitor acute exercise and chronic exercise, respectively.