Dielectrophoretic differentiation of mouse ovarian surface epithelial cells, macrophages, and fibroblasts using contactless dielectrophoresis

Ovarian cancer is the leading cause of death from gynecological malignancies in women. The primary challenge is the detection of the cancer at an early stage, since this drastically increases the survival rate. In this study we investigated the dielectrophoretic responses of progressive stages of mouse ovarian surface epithelial (MOSE) cells, as well as mouse fibroblast and macrophage cell lines, utilizing contactless dielectrophoresis (cDEP). cDEP is a relatively new cell manipulation technique that has addressed some of the challenges of conventional dielectrophoretic methods. To evaluate our microfluidic device performance, we computationally studied the effects of altering various geometrical parameters, such as the size and arrangement of insulating structures, on dielectrophoretic and drag forces. We found that the trapping voltage of MOSE cells increases as the cells progress from a non-tumorigenic, benign cell to a tumorigenic, malignant phenotype. Additionally, all MOSE cells display unique behavior compared to fibroblasts and macrophages, representing normal and inflammatory cells found in the peritoneal fluid. Based on these findings, we predict that cDEP can be utilized for isolation of ovarian cancer cells from peritoneal fluid as an early cancer detection tool.     

Developing and sustaining a sense of place: The role of social information

This exploration of the role of information in helping people to develop and sustain feelings of belonging, or a sense of place, is based on a study that investigated the reasons for the high population turnover in the Northern Territory of Australia. The Northern Territory along with Darwin, its capital, has a number of unusual characteristics including geographical isolation. A key component of the theoretical framework for the research focuses on social information, which has similarities to the more commonly termed everyday life information. The specific concern is with the kind of information that helps people connect to a community and is clearly linked with social networking. The sample for the major empirical component for the article was recruited at Darwin's Mindil Beach Market. In telephone phone interviews with those who had agreed to participate, participants discussed how they learned about Darwin and the Northern Territory, and their views of Darwin as a place to live. Interpersonal contacts, including at the Market and at special events, were most often mentioned in relation to information, confirming literature findings on the importance of family and friends as an information source. Other sources were newspapers and physical markers around the City of Darwin. The conclusion is that the processes of social networking, which work well for some residents, could be enhanced through the use of information grounds, a concept widely discussed in the library and information science literature. Obvious information grounds in Darwin, where the usual processes could be enhanced by the distribution of information by formal information providers, include Mindil Beach Market and the special events for which Darwin is renowned.     

Higher education curriculum orientations and the implications for institutional curriculum change

This research is based on an empirical study exploring how academics make curriculum decisions and their perceptions of the influences that shape their decisions. Interviews were held with 20 academics from diverse disciplines, who were both research active and committed to teaching. The higher education curriculum was conceptualised as a field of decision-making shaped by academics’ beliefs about educational and contextual influences. The study identified five distinctive curriculum orientations representing coherent patterns of curriculum decisions aligned with academics’ beliefs about educational purposes. Case studies are presented to elucidate each of the curriculum orientations. Curriculum orientations were also found to shape academics’ responses to educational change. The following higher education change drivers are explored: graduate employability and the skills agenda, teaching–research relationships, changing understandings about teaching and learning, educational technologies and flexible delivery. The findings suggest implications for institutional curriculum change initiatives and academic development programmes.     

Using learning principles as a theoretical framework for instructional consultations

Learning theory provides a powerful framework for analyzing instructional consultations and increasing their effectiveness. In this paper, the authors review the main tenets of learning theory, apply them to consultation scenarios, and present a complex case study to show how they can guide analysis and problem solving around challenging consultations.     

Androgyny and the Mentoring Role: an empirical study to examine for prominent mentor expectations

abstract

As part of an ongoing research design, we wished to examine how mentors behave whilst in the mentoring role. The starting point for this was a study to examine how mentors felt they were expected to behave in context; that is, an organised educational mentoring situation, as we recognised the importance of situation‐specificity. Prior to empirical study, we theorised that the mentor may be androgynous; or more precisely are able to display high levels of expressive and instrumental behaviour. Seven suppositions were examined. Our findings were that whilst in the role, mentors do indeed feel expected to blend and display high levels of both instrumentality and expressiveness. Further, the levels of expressive behaviour they perceived they were expected to display were significantly higher. The higher levels of expected expressive behaviour is not congruent with the androgyny literature: our samples proved an exception to the ‘masculine supremacy effect’. Also, that mentoring may require high levels of stereotypically feminine behaviour is something that the mass of mentoring literature has not discussed and may warrant further attention. We concluded that the diverse, complex and complicated nature of mentoring is met with expectations to display flexibility and versatility across situations — to take a proactive approach to coping with mentoring situational demands — such is not mitigated by gender. Their reported high expected levels of both expressive and instrumental behaviour are congruent with our theory of the androgynous mentor.     

Student‐teachers' perspectives on positive and negative social processes in school

In recent years, the Standards for Qualified Teacher Status in England have placed new emphasis on student‐teachers' ability to become integrated into the ‘corporate life of the school’ and to work with other professionals. Little research, however, has been carried out into how student‐teachers perceive the social processes and interactions that are central to such integration during their initial teacher education school placements. This study aims to shed light on these perceptions. The data, gathered from 23 student‐teachers through interviews and reflective writing, illustrate the extent to which the participants perceived such social processes as supporting or obstructing their development as teachers. Signals of inclusion, the degree of match or mismatch in students' and school colleagues' role expectations, and the social awareness of both school and student‐teacher emerged as crucial factors in this respect. The student‐teachers' accounts show their social interactions with school staff to be meaningful in developing their ‘teacher self’ and to be profoundly emotionally charged. The implications for mentor and student‐teacher role preparation are discussed in this article.     

Integrating technical editing students into a multidisciplinary engineering project

A three‐year experiment in integrating technical editing students into a multidisciplinary engineering design project developed several ways of helping students apply classroom learning to practical problems. Each year, the engineering students formed Integrated Product Teams (IPTs) and the technical editing students provided editorial support, first as full members of IPTs, then as separate editorial support teams. Research from cooperative learning and teamwork indicates strategies and techniques for best integrating the technical editing students.     

Gender and Ethnicity Among Community College Students: The Relationship Between Adlerian Themes

This study explored the relationship between Adlerian themes, gender, and ethnicity. Results indicate significant differences between Hispanic and Caucasian (non-Hispanic) community college students in Belonging and Social Interest as measured by the BASIS-A. Differences between males and females in Going Along and Wanting Recognition are also explored. The findings are discussed in relation to college students, teaching, and classroom climates.