The development of the Polymerase Chain Reaction (PCR) has been a major technical milestone in molecular genetics with the greatest impact on research, in rapid medical diagnosis and in studying the pathogenesis of disease. This study highlights the practical applications of polymerase chain reaction in diagnosis and includes a recent application of PCR in differentiating polio from other nonpolio enteroviruses. 相似文献
Psychological safety is the shared belief that the team is safe for interpersonal risk-taking. Critical team events such as conflict and faultlines (hypothetical lines that split a team into subgroups) should impact psychological safety. Previous research has shown the benefits of task conflict on team outcomes under certain conditions and the consistently negative effects of demographic faultline strength on team outcomes. We propose that being close to the principal (what we call belonging to the principal’s in-group) should help attenuate the negative effects of task conflict and faultlines among teachers, because it is associated with a more effective working relationship. In a survey study with 244 teachers from 45 primary schools, we tested the moderating effect of belonging to the principal’s in-group vs. belonging to an out-group on the relationships of task conflict and faultlines on psychological safety. Results of multilevel model tests showed that relationship conflict (but not task conflict) and faultlines decreased psychological safety. However, when teachers belonged to the principal’s in-group as compared to an out-group, task conflict had no negative effect on psychological safety. We present implications to bridge the negative effects due to subgroup presence, so that psychological safety can become a resource for all. 相似文献
Over the past two decades, comprehensive sexuality education has increasingly been recognised as a measure that positively impacts on the sexual behaviour of young people in Africa. Despite this, and a political call to scale-up the use of comprehensive sexuality education in schools in South Africa, learners with disabilities continue to be left behind. Besides contending with negative hegemonic constructs of disabled sexualities, educators of learners with disabilities lack skills and resources to teach sexuality in accessible formats. Based on this premise, a comprehensive sexuality education approach – Breaking the Silence – was developed and piloted to assist educators of learners with disabilities to provide access to comprehensive sexuality education in KwaZulu-Natal, South Africa. This article presents results from the formative evaluation of this pilot work and discusses educators’ perceptions of their learners with intellectual disabilities’ sexual knowledge, agency and behaviour after implementing the approach. Although educators appeared to situate learners with intellectual disabilities as sexual agents, their implementation of the approach was dependent on the cognitive ability of learners, and discourses of culture, gender and protection from violence. 相似文献
The authors used transcendental phenomenology to explore the campus interview experiences and diversity concerns of counselor education faculty from underrepresented populations. Six themes were identified: issues of integrity, disappointment in the counseling profession, importance of authenticity, intersectionality of major identity markers, competence, and supportive experiences. Findings suggest that culturally competent search committees should be aware of biases and policies promoting institutional and systemic discrimination. 相似文献
This study assessed the efficacy of a semester-long buddy project in facilitating intercultural friendship development. Results of pre-, post-, and follow-up surveys show significant improvements in knowledge and attitude; correlations between post-project knowledge, attitude, and interest in continued contact; and correlations between interest in continued contact and actual contact in the follow up. The majority of the participants evaluated the project as effective for friendship formation. Of the follow-up respondents, 23% developed friendships with their former buddies. The project serves as an example for assignments with the potential for friendship development, which is essential for optimal intergroup contact. 相似文献
This paper describes a partnership between a university and an urban school district, formed with a goal of preparing mathematics teacher leaders to conduct professional development (PD) at their schools. The university and district partners worked together to achieve the district’s mission of providing every student with high-quality instruction and equitable learning opportunities in mathematics by building the district’s capacity to conduct school-based PD for mathematics teachers. Given the power of school-based subject-specific PD for improving instructional quality, we worked with Teacher Leaders from participating schools to prepare and support them to lead PD workshops at their schools. In this paper, we examine how Teacher Leaders learn and adapt key elements of a PD model over three school years through the lenses of Prediger et al.’s Three-Tetrahedron-Model (2019) and the university’s Learning to Lead model. Over 3 years, we see that Teacher Leaders use the key structures of the PD model; make adaptations in response to school goals, interests, and priorities; and gain confidence in their work with colleagues. By viewing the adaptations through the lens of pedagogies of practice as well as the relationships illustrated by the 3-T model, this work offers insights into the complexities of teacher leadership development.
The aim of this study was to establish a graded exercise test protocol for determining the peak physiological responses of female outrigger canoeists. Seventeen trained female outrigger canoeists completed two outrigger ergometer graded exercise test protocols in random order: (1) 25 W power output for 2 min increasing by 7.5 W every minute until exhaustion; and (2) 25 W power output for 2 min increasing by 15 W every 2 min to exhaustion. Heart rate and power output were recorded every 15 s. Expired air was collected continuously and sampled for analysis at 15-s intervals, while blood lactate concentration was measured immediately after and 3, 5, and 7 min after exercise. The peak physiological and performance variables examined included peak oxygen uptake (VO2peak), minute ventilation, tidal volume, ventilatory thresholds 1 and 2, respiratory rate, respiratory exchange ratio, heart rate, blood lactate concentration, power output, performance time, and time to VO2peak. There were no significant differences in peak physiological responses, ventilatory thresholds or performance variables between the two graded exercise test protocols. Despite no significant differences between protocols, due to the large limits of agreement evident between protocols for the peak physiological responses, it is recommended that the same protocol be used for all comparison testing to minimize intra-individual variability of results. 相似文献
Purpose: To evaluate the reliability and validity of the six-minute walk test (6MWT) with respect to its ability to predict functional capacity in patients with chronic heart failure. Methods: A systematic review was performed via 8 databases to assess relevant English language full-text articles published from January 1, 1980 to October 31, 2009. Participant characteristics, interventions, reliability, validity, and predictive value for each article with respect to functional capacity as defined by peak VO2 levels were extracted and compared. Quality Assessment of Diagnostic Accuracy Studies (QUADAS) scores were determined for each study. Results: Fourteen studies met the selection criteria. Comparison of the studies investigating reliability shows that the 6MWT has good reproducibility. The 6MWT demonstrates moderate correlation with peak VO2 levels, and ability to predict VO2 (functional capacity) dependent on distance walked. Cut-off distances vary from 300 to 490 meters depending on the study; if total distance walked remains equal or less than these values, the 6MWT retains its strong predictive value. Conclusion: The 6MWT has good reliability, moderate validity, and a significant ability to predict functional capacity in patients with CHF who do not walk greater than 490 meters.Key Words: six-minute walk test, chronic heart failure, functional capacity, peak VO2相似文献
Global Learning and Observations to Benefit the Environment (GLOBE) is an international environmental education and science partnership which coordinates the work of students (aged 5 to 18), teachers and scientists from 48 countries on five continents to study and better understand the global environment. Accurate ground reference data is fundamental to the use of remotely sensed data for land cover classification and mapping. Because very little ground reference data has been collected, the accuracy of many land cover maps may be questioned, thus accurate land cover ground reference data is an important need that could be addressed through GLOBE scientist-student collaboration. If earth systems scientists are to use student data, it is important that those data be as accurate as possible to ensure reliability of research results. Thus a key question for this research is whether student collected data are accurate enough to support rigorous scientific investigations. This paper describes results of the GLOBE Science-Education Team on Data Validation and Accuracy Assessment's collaboration with teachers and students to: (1) design and test the pre-protocol learning activities; (2) test the protocols intended to guide the collection and analysis of data; and (3) implement the learning activities and protocols to determine the relative accuracy of student collected versus professionally collected land cover data. To ensure the most accurate classification of land cover possible, a new international hierarchical land cover classification system, the Modified Unesco Classification (MUC) system was developed. GLOBE Data Collection Protocols and methods were designed and implemented to test the accuracy of student collected reference data were designed and implemented. Students who collected land cover reference data using GLOBE protocols, obtained data which are at least as accurate as that collected by professionals. 相似文献