Potential immunomodulation effect of the extract of Nigella sativa on ovalbumin sensitized guinea pigs

Several different pharmacological effects have been described for Nigella sativa (Siah-Daneh), including an anti-inflammatory effect. In the present study, the effect of the extract of N. sativa on lung pathology and blood interleukin-4 (IL-4) and interferon-γ (IFN-γ) of sensitized guinea pigs was examined. Three groups (n=8 for each group) of guinea pigs sensitized to ovalbumin (OA) were given drinking water alone, and drinking water containing low and high concentrations of the plant extract, respectively. The animals of the control group (n=8) were treated with saline instead of OA and were given drinking water. The pathological changes of the lung, including infiltration of eosinophils and lymphocytes, local epithelial necrosis, the presence of oedema, thickening of the basement membrane, smooth muscle layer hypertrophy, mucosal secretion, and the presence of mucosal plug, and blood IL-4 and IFN-γ of sensitized guinea pigs were evaluated. The lungs of the sensitized group showed significant pathological changes (P<0.001). Blood IL-4 and IFN-γ were increased in sensitized animals compared to the controls (P<0.01 and P<0.001, respectively). Treatment of sensitized animals with the extract led to a significant decrease in pathological changes of the lung (P<0.01 to P<0.001), except for the oedema in the sensitized group treated with low concentration of the extract, but an increased IFN-γ. These results confirm a preventive effect of N. sativa extract on lung inflammation of sensitized guinea pigs.     

Google glass adoption in the educational environment: A case study in the Gulf area

Education and Information Technologies - The rapid changes in recent years witnessed the development of technology-based education environment where teachers and learners can interact by adopting...     

Effect of ocimum flavonoids as a radioprotector on the erythrocyte antioxidants in oral cancer

Flavonoids extracted from the leaves of Indian holy basil, Ocimum sanctum showed promising results as radioprotector in rodents. Hence it was thought pertinent to analyze the antioxidants of erythrocytes in oral cancer patients who were concurrently treated with radiation and ocimum flavonoids. Oral cancer patients consisted of 2 groups. Group A (n=17) received radiation alone while Group B(n=17) received radiation and ocimum flavonoids(OF). Samples of heparinised blood were collected prior to treatment, 15 and 30 days respectively after treatment. Blood from normal healthy volunteers were taken as controls (n=25). Erythrocytes were analyzed for the antioxidants viz. glutathione, glucose 6 phosphate dehydrogenase, glutathione peroxidase and superoxide dismutase by the standard methods. Results of analysis indicated that erythrocytes from cancer patients (Group A and B) had significantly high glutathione levels before as well as after treatment compared to controls. It was observed that Group B which received OF showed a significant reduction in glutathione levels in comparison with Group A. All the other parameters showed no statistical significant changes. Results of the study suggest that erythrocytes from cancer patients responded to oxidative stress by elevating glutathione levels, while a decrease in glutathione levels observed in Group B, could be due to the free radical scavenging effect of OF, sparing the glutathione. However OF did not seem to exert its effect on other antioxidants of erythrocytes.     

Some reflections on learning from early school experience

This paper is a report of a study on early school experience as part of a Teacher Education programme in Turkey. The programme requires second-year students to act as students only, observing their mentors in classrooms rather than practising teaching so that they can gain a wider view of the teaching profession. The study analyses the experiences of these students, therefore, particularly focusing on the extent to which this scheme is conducive to making students learn from their experience. The findings from the qualitative data suggest that careful attention must be paid to major factors shaping this process, such as the school atmosphere as a whole, the nature of tasks for observation, the quality of interaction between student-mentor, and, more importantly, the opportunities for students to develop an inquiry-based approach to teaching.     

Librarian integration in a four-year medical school curriculum: a timeline

The Taubman Health Sciences Library (THL) is integrated in all four years of the University of Michigan Medical School (UMMS) curriculum. Information resources are introduced at strategic points throughout the curriculum so that students receive training at times when they are most likely to need the resource. Most of the core instruction sessions are taught in teams that consist of librarians and UMMS faculty, which provides unique learning opportunities for students. This article describes each THL instruction activity in the four-year undergraduate UMMS curriculum and provides commentary on the overall effectiveness of this integrated approach to instruction.     

Review on COVID-19 Etiopathogenesis,Clinical Presentation and Treatment Available with Emphasis on ACE2

In December 2019, Wuhan city in the Hubei province of China reported for the first time a cluster of patients infected with a novel coronavirus, since then there has been an outburst of this disease across the globe affecting millions of human inhabitants. Severe acute respiratory syndrome coronavirus type-2 (SARS-CoV-2), is a member of beta coronavirus family which upon exposure caused a highly infectious disease called novel coronavirus disease-2019 (COVID-19). COVID-19, a probably bat originated disease was declared by World Health Organization (WHO) as a global pandemic in March 2020. Since then, despite rigorous global containment and quarantine efforts, the disease has affected nearly 56,261,952 laboratory confirmed human population and caused deaths of over 1,349,506 lives worldwide. Virus passes in majority through respiratory droplets and then enters lung epithelial cells by binding to angiotensin converting enzyme 2 (ACE2) receptor and there it undergoes replication and targeting host cells causing severe pathogenesis. Majority of human population exposed to SARS-CoV-2 having fully functional immune system undergo asymptomatic infection while 5–10% are symptomatic and only 1–2% are critically affected and requires ventilation support. Older people or people with co-morbidities are severely affected by COVID-19. These categories of patients also display cytokine storm due to dysfunctional immune response which brutally destroys the affected organs and may lead to death in some. Real time PCR is still considered as standard method of diagnosis along with other serology, radiological and biochemical investigations. Till date, no specific validated medication is available for the treatment of COVID-19 patients. Thus, this review provides detailed knowledge about the different landscapes of disease incidence, etiopathogenesis, involvement of various organs, diagnostic criteria’s and treatment guidelines followed for management of COVID-19 infection since its inception. In conclusion, extensive research to recognize novel pathways and their cross talk to combat this virus in precarious settings is our future positive hope.     

COVID-19 and Gut Microbiota: A Potential Connection

Currently, world is facing a global outbreak causing a pandemic threat known as COVID-19. This infectious disease is triggered by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). Gut microbiota harbours multi species community with a strong impact on host immune homeostasis. However, our knowledge about this gut microbiota and its symbiotic relationship with immune activation in association with SARS-CoV-2 is limited. Unbalanced bacterial flora with too many opportunistic infections can shift immune system towards a cascade of inflammatory responses leading to multi organ damage. This review will highlight immune-regulation via various mechanisms in SARS-CoV-2 infection. Diet has an unbelievable influence on gut microbiome that allows a new state of homeostasis to be reached through timing, frequency and duration of intake. This review article focuses on gut, lung microbiota and immunomodulation with specific attention on immune activation by gut microbiota.     

Real-time,evidence-based medicine instruction: a randomized controlled trial in a neonatal intensive care unit

Purpose: The study assesses potential for improving residents'' evidence-based medicine searching skills in MEDLINE through real-time librarian instruction.Subjects: Ten residents on a rotation in a neonatal intensive care unit participated.Methodology: Residents were randomized into an instruction and a non-instruction group. Residents generated questions from rounds and searched MEDLINE for answers. Data were collected through observation, search strategy analysis, and surveys. Librarians observed searches and collected data on questions, searching skills, search problems, and the test group''s instruction topics. Participants performed standardized searches before, after, and six-months after intervention and were scored using a search strategy analysis tool (1 representing highest score and 5 representing lowest score). Residents completed pre- and post-intervention surveys to measure opinions about MEDLINE and search satisfaction.Results: Post-intervention, the test group formulated better questions, used limits more effectively, and reported greater confidence in using MEDLINE. The control group expressed less satisfaction with retrieval and demonstrated more errors when limiting. The test and control groups had the following average search scores respectively: 3.0 and 3.5 (pre-intervention), 3.3 and 3.4 (post-intervention), and 2.0 and 3.8 (six-month post-intervention).Conclusion: Data suggest that measurable learning outcomes were achieved. Residents receiving instruction improved and retained searching skills six-months after intervention.     

Evaluating biometrics for online banking: The case for usability

Rising cyber-crimes have heightened existing concerns about e-banking security. However currently the use of biometric technology in the banking sector is not prevalent. We developed a biometric system for authenticating e-banking and applied the established System Usability Scale (SUS) to evaluate its effectiveness from the perspective of potential users. The case demonstrates that on the whole users are very favourable towards a biometric banking system and ostensibly found the system developed usable. We found that when evaluating the biometric system, its different components needed to be considered – namely the biometric technology and the institutional process that supports verification and authentication.     

Study of enzyme activities in the descending part of the duodenum in patients of duodenal ulcer

The aim of this study was to measure the levels of lactase, sucrase, maltase, leucine amino peptidase and gamma-glutamyl transpeptidase in duodenum of Indian patients with duodenal ulcer. The effect of duodenum inflammation on these brush border enzymes has also been analysed in this study. Levels of lactase, sucrase, maltase, leucine amino peptidase and gamma-glutamyl transpeptidase were assessed in duodenal biopsies of 20 patients of duodenal ulcer and 20 non-ulcer dyspepsia. The duodenal biopsy specimens were also examined histopathologically for presence or absence of inflammation. There was no significant difference (p>0.05) in the activity of above mentioned enzyme levels in both the groups. Only levels of gamma-glutamyl transpeptidase were significantly decreased in patients of duodenal ulcer with duodenal inflammation. This study shows that only the levels of gamma-glutamyl transpeptidase were significantly decreased in patients of duodenal ulcer with inflammation but no change in duodenal enzymes due to duodenal ulcer as compared to non-ulcer dyspepsia.