Reciprocal Teaching of Comprehension‐fostering and Comprehension‐monitoring Activities with Ten Primary School Girls

Abstract This paper reports an experimental evaluation of a direct instruction procedure in which children are taught and practise activities to both foster and monitor their comprehension while reading. Three different class groups of low comprehenders, Standard 4, Form 1 and Form 2, were serially exposed to 21 days of 20‐25 minutes instruction in four study activities: summarising, questioning, clarifying and predicting. Instruction was by reciprocal teaching whereby the adult tutor and children took turns leading a dialogue centred around the important topics of the instructional text. The design of this study incorporated both with‐subjects multiple baseline and between‐subjects comparisons, the no treatment comparison groups being average and above average comprehending class peers. Daily comprehension assessment on a different text at the same difficulty level as that used for reciprocal teaching and pre and post intervention scores on a reading comprehension test served as the dependent measures for the study. Results show significant increases in accuracy on comprehension tests for all experimental groups upon introduction of the reciprocal teaching procedure. On completion of the intervention two of these groups scores matched those of the above average controls. Follow‐up probes eight weeks later showed that all groups had maintained their comprehension gains.     

Racial Identity and Psychological Symptoms Among African Americans Attending a Historically Black University

Previous research found racial identity predictive of psychological distress among African American students at predominantly White colleges. This study examined these relationships among 154 African American undergraduates attending a historically Black university. Racial identity was independent of psychological distress, suggesting that African American students' racial identity predicts psychological distress only in settings in which they are the minority.     

PERSONALITY PROFILES AND SELECTION FOR COURSES

16PF Personality profiles for 364 young civil engineers have been compared to the British Norms for the 16PF Questionnaire. Civil Engineers differ from the British Norms on five of the 20 factors derived from the Questionnaire. These factors indicate the questions on which to concentrate when selecting students for civil engineering courses.     

DIAGNOSTIC TESTS AND EXAMINATION RESULTS

The possibility of using diagnostic tests was explored and several tests were used with civil engineering students to see if they would be useful in selecting students for the civil engineering degree course. The performance of students on the tests used was compared with the examination results and it was found that the tests showed a little promise. It will be necessary to design a test specifically for civil engineering students. On the other hand perhaps our methods of teaching and examining subjects fundamental to civil engineering should be examined in depth. The results from the tests used indicated a culture gap between home and overseas students and the need for a database of student information.     

Psychometric Properties of the Full and Short Versions of the Quality of Life Instrument Package: Results from the Ontario province-wide study

Results that bear upon the psychometric properties of the Quality of Life Instrument Package are presented. The reliability and validity of quality of life data from a probability sample of 500 persons with developmental disabilities in Ontario were assessed. The data indicate that most of the instruments found in the package met acceptable psychometric standards. In some cases, specific domain scores need to be interpreted with caution. The Full Version of the instrument seems appropriate for ongoing practical program planning with clients. The Short Version seems especially appropriate for evaluation and monitoring purposes.     

Herceptin functionalized microfluidic polydimethylsiloxane devices for the capture of human epidermal growth factor receptor 2 positive circulating breast cancer cells

Building on recent breakthroughs in the field of microfluidic-based capture of rare cancer cells circulating in the blood, the present article reports on the use of Herceptin functionalized PDMS devices designed to efficiently capture from blood cancer cells, overexpressing the tyrosine kinase human epidermal growth factor receptor (HER2). The identification of patients overexpressing HER2 is critical as it typically associates with an aggressive disease course in breast cancer and poor prognosis. Importantly, HER2 positive patients have been found to significantly benefit from Herceptin (Trastuzumab), a humanized monoclonal antibody (MAb) against HER2. Disposable PDMS devices prepared using standard soft lithography were functionalized by the plasma polymerization of an epoxy-containing monomer. The epoxy-rich thin film (AGEpp) thus created could be conjugated with Herceptin either directly or through a polyethylene glycol interlayer. The properties and reactivity toward the monoclonal antibody conjugation of these coatings were determined using x-ray photoelectron spectroscopy; direct conjugation provided a good compromise in reactivity and resistance to biologically nonspecific fouling and was selected. Using the breast cancer cell line SK-BR-3 as a model for cells overexpressing HER2, the immunocapture efficacy of the Herceptin functionalized PDMS was demonstrated in model studies. Validation studies confirmed the ability of the device to efficiently capture (～80% capture yield) HER2 positive cells from full blood.     

Polyelectrolyte multilayer surface functionalization of poly(dimethylsiloxane) (PDMS) for reduction of yeast cell adhesion in microfluidic devices

Polyelectrolyte multilayers (PEMs) based on the combinations poly(diallyldimethylammonium chloride)∕poly(acrylic acid) (PDADMAC∕PAA) and poly(allylamine hydrochloride)∕PAA (PAH∕PAA) were adsorbed on poly(dimethylsiloxane) (PDMS) and tested for nonspecific surface attachment of hydrophobic yeast cells using a parallel plate flow chamber. A custom-made graft copolymer containing poly(ethylene glycol) (PEG) side chains (PAA-g-PEG) was additionally adsorbed on the PEMs as a terminal layer. A suitable PEM modification effectively decreased the adhesion strength of Saccharomyces cerevisiae DSM 2155 to the channel walls. However, a further decrease in initial cell attachment and adhesion strength was observed after adsorption of PAA-g-PEG copolymer onto PEMs from aqueous solution. The results demonstrate that a facile layer-by-layer surface functionalization from aqueous solutions can be successfully applied to reduce cell adhesion strength of S. cerevisiae by at least two orders of magnitude compared to bare PDMS. Therefore, this method is potentially suitable to promote planktonic growth inside capped PDMS-based microfluidic devices if the PEM deposition is completed by a dynamic flow-through process.