粉防己碱对VSMC表型转化和p38及MKP-1表达的影响

研究粉防己碱(tetrandrine,Tet)抑制内膜损伤后血管平滑肌细胞(VSMC)表型转化和对p38丝裂原激活蛋白激酶(p38MAPK)及丝裂原激活蛋白激酶磷酸酶-1(MKP-1)表达的影响。采用HE染色检测28 d假损伤组(S组)、损伤组(I组)和损伤 Tet治疗组(Tet组)血管形态学改变;分别使用免疫组化、Western blot和RT-PCR技术检测I组和Tet组7、14、28 d增殖细胞核抗原(PCNA)、平滑肌α-肌动蛋白(SMα-actin)、p38 MAPK和MKP-1表达的变化。结果为:①28天S组血管壁各层结构完整;I组新生内膜(NI)面积显著增加,管腔面积(LA)显著缩小;Tet组NI增殖较I组明显减轻,LA增加。②损伤后7 d,Tet组与I组之间血管壁SMα-actin、PCNA、p38 MAPK和MKP-1表达基本一致,NI增殖程度亦基本相同。Tet组14和28 d,血管壁中PCNA和p38 MAPK表达均低于I组;而MKP-1表达均高于I组;14、28 d SMα-actin表达均略高于I组。因此Tet可不同程度地拮抗内膜损伤后P38MAPK信号转导途径及其调节,抑制VSMC表型转化,继而减缓NI增殖。     

支气管动脉灌注治疗肺癌疗效与靶血管及病理学类型的关系

目的:探讨动脉导管灌注治疗肺癌的疗效与其血管及病理学类型的关系。方法:对100例肺癌患者进行选择性支气管动脉造影,造影确定后行支气管动脉化疗药物灌注术。结果:100例肺癌患者中富血管型肺癌占81%,乏血管型占18%,无血管型占1%。富血管型中未分化癌占80.25%,鳞癌占16%,腺癌占3.75%。乏血管型腺癌占66.66%,无血管型疗效最差。结论:支气管动脉灌注治疗肺癌,富血供中未分化癌疗效最佳。     

Primary leiomyosarcoma of the nipple-areola complex： Report of a case and review of literature

Primary leiomyosarcoma of the nipple-areola complex is extremely rare. Less than ten such cases have been reported in English literature so far. Herein we describe a 52-year-old female presenting with a 1.5 cm× 1.1 cm×0.7 cm nodular lesion over her left nipple, and leiomyosarcoma was proved by pathological examination of the excised specimen. Positron emitted tomogram (PET) revealed no abnormal signal other than the primary site. Microscopically, this poorly circumscribed tumor was composed of interlacing bundles of smooth muscle cells with bizarre and pleomorphic nuclei, as well as prominent nucleoli. Its mitotic count was up to 7 mitoses per 10 high power fields (HPF). Immunohistochemical study of tumor cells revealed positive stain for α-smooth muscle actin and vimentin; and negative for cytokeratin, CD34 and S-100. Left simple mastectomy was undertaken and no residual mass lesion was noted on the resected specimen. Related literatures about the diagnosis and treatment for breast leiomyosarcoma will be presented here.     

康复体育对老年支气管哮喘患者疗效的观察与分析

为探讨康复体育对老年支气管哮喘患者的治疗效果及机制，将57名老年支气管哮喘患者随机分为实验组（进行1年系统的康复体育锻炼）和对照组（除未进行康复体育锻炼外。其他条件与实验组相同），观察测定锻炼前后心肺机能相关指标和支气管哮喘发病次数、发病时症状表现．结果显示：实验组心肺功能能力明显增强。支气管哮喘发病次数显著性下降，发病时症状减轻；与实验前和对照组比较有显著性差异．研究表明，康复体育能增强老年支气管哮喘患者心肺功能能力，提高抗病能力，改善哮喘病发病症状，对哮喘病具有明显康复疗效．     

婴幼儿氨茶碱中毒抢救分析

氨茶碱系支气管扩张药 ,在临床上广为应用 ,如使用不当常常引起中毒 ,现将某医院近年来出现的4例氨茶碱中毒病例进行分析如下 :1　临床资料1 1　一般资料 :4例患儿 ,男 3例 ,女 1例。年龄分别为 3个月、4个月、10个月、1岁 5个月。原发病其中 2例 ,诊断为毛细支气管炎 ,2例诊断为哮喘支气管肺炎。 4例病人中 3例为某院住院病人在输液治疗过程中出现中毒症状 ,1例在医院外治疗中出现中毒症状 ,中毒原因均属治疗护士在配药过程中计算错误而引起。症状 :3例在某医院住院的患儿均在静脉输液中突然出现烦燥 ,哭吵不安 ,面色苍白 ,呼吸急促 ,全身…     

Effect of serum concentration on adhesion of monocytic THP-1 cells onto cultured EC monolayer and EC-SMC co-culture

Background:The adhesion of monocytes to the endothelium following accumulation oflow-density lipoprotein(LDL) in subendothelial spaces is an important step in the development of intimal hyperplasia in arterially implanted vein grafts and atherosclerosis in both animals and humans.However.it iS not well known how serum factors affect the adhesion of monocytes.Methods:We have studied the efrect of fetal calf serum(FCS).which we considered a source of LDL.on the adhesion of monocytes to endothelial cells(Ecs)by using human monocytic THP-1 cells and both a monolayer of cultured bovine aortic endothelial cells(EC monoculture)and a co-culture with bovine aortic smooth muscle cells(EC-SMC co-culture).Results:It was found that the addition of FCS to the medium greatly affected the adhesion of THP-1 cells,and the higher the concentration of FCS in the medium,the greater the adhesion of THP-1 cells to endothelial cells.Adhesion of THP-1 cells to an EC-SMC co-culture Was approximately twofold greater than that to an EC monoculture,and after adhering to endothelial cells,many THP-1 cells transmigrated into the layer of smooth muscle cells.Conclusion:The results suggest that the elevation of the LDL(cholesterol)level in blood provides a favorable condition for the development of intimal hyperplasia and atherosclerosis by promoting the adhesion of monocytcs to the endothelium and their subsequent migration into subendothelial spaces.     

小儿哮喘的防治措施

支气管哮喘可以发生在各种年龄,但大部分均在童年起病。因此积极防治儿童哮喘具有极其重要的意义。 1．目前治疗哮喘最安全有效的是吸入激素疗法,可以减轻     

缓释片药物不可掰半服用

近年来上市的控释、缓释片剂药物越来越多。因为它能起到长效作用,减少服药次数,给病人服药带来了方便。如氨茶碱缓释片（常欣）是治疗中老年支气管哮喘、肺心病等疾病的常用药物;硝苯地平（心痛定）缓释片则常用于治疗高血压、冠心病;吗啡控释片常用于晚期癌症的口服止痛。但不少人尤其是老年人在服用这些药物时为了便于服用常将药片掰成几半,其实这是不科学的。     

有些药物不可贸然停

李先生在无明显诱因的情况下突然昏迷,家人发现后紧急送到医院。接诊医生在仔细查体问诊后发现,原来李先生因支气管哮喘服用了强的松（糖皮质激素类药）,病情好转后擅自停药而导致昏迷。为何停药会引发如此大的风险呢？     

永恒的悲哀

正6月4日晴2011年9月27日凌晨5:00,正在我们迎接国庆的时候,顾老师的妻子却结束了她的人生历程,前往另一个世界。10月2日,无锡市殡仪馆,她的亲人、朋友、同事前来向她道别。花圈如海,泪如雨下。遗像上顾老师的妻子——费英带着微笑在看着我们。她脸色红润,神态安详,眼睛里流露出留恋的目光,可能是她舍不得她认识的每一个人,特别是她的儿子——顾凯汶。在顾老师妻子已经定格的微笑中,我们读出了她永恒的悲哀。费英阿姨本来就有心脏病,随后又患上支气管扩张。她撑不住了,终于倒下了。顾