符仲华:上工少涉 诚朴勤仁

很难找到这样的医生:拥有中医学士、硕士学位和西医博士学位,同时有博士后经历,却在南京繁华大街的办公楼里自己开一家诊所,他只用自己发明的浮针疗法给患者治病,其他方法统统不用。更奇特的是:这个医生在看病的同时,还不断写书,写论文,不断带学生,不断讲学。这个人就是符仲华。在外行人看来,符博士治病是一个非常神奇的过程:用一次性浮针在病痛周围的皮下进行横向来回牵拉和局部疏松组织的扫散动作,再配合再灌注活动,     

运动对心肌损伤保护作用的若干研究进展

缺血性损伤是造成心肌细胞死亡和心脏功能紊乱的常见诱因,主要是因为缺血时心肌供血不足导致不能满足能量代谢的需要,严重时会引起心肌梗塞。近年来大量实验研究表明,运动对心肌损伤具有保护作用,阐明运动对抗心肌缺血性损伤的保护机制有助于预防和治疗心肌损伤。     

DOMS发生机制与中医防治研究进展分析

延迟性肌肉酸痛(delayed-onset muscle soreness,DOMS)是指肌体进行大运动量后,特别是强度突然增加或进行新的不习惯的运动之后,一段时间所出现的肌肉酸痛现象.其产生机制有:机械性损伤学说、肌肉痉挛学说、炎症反应学说、缺血再灌注损伤假说.还着重介绍了按摩、针灸和中药等中医防治方法研究现况.     

钻孔灌注桩施工中的质量问题及防治办法

本文主要从施工的角度,全面阐述钻孔灌注桩在钻孔过程中及水下砼灌注过程中出现的主要施工质量问题,并结合工地实践提出相应的防治办法.     

钻孔灌注桩断桩产生原因、预防及处理措施

简略地论述了钻孔灌注施工产生断桩的原因、表现形式及其危害,并提出了预防和处理断桩的方法。     

A novel way of liver preservation improves rat liver viability upon reperfusion

Background/aim： Currently, the liver is cold-preserved at 0-4 ℃ for experimental and clinical purposes. Here, we investigated whether milder hypothermia during the initial phase of the preservation period was beneficial for liver viability upon reperfusion. Methods： In the first set of experiments, rat livers were preserved either conventionally in clinically used histidine-trypthopan-ketoglutarate （HTK） solution （Group A： 45 min and Group B： 24 h） or by slow cooling HTK solution （from 13 ℃ to 3 ℃） during the initial 45 min of preservation （Group C： 24 h）. In the second set of experiments, additional groups of livers were evaluated： Group BB-preservation according to Group B and Group CC-preservation according to Group C. Further, some livers were preserved at 13 ℃ for 24 h. Livers were then reperfused using a blood-free perfusion model. Results： Bile production was approximately 2-fold greater in Group C compared to Group B. Alanine transaminase （ALT） and aspartate transaminase （AST） release into perfusate were 2-3-fold higher in Group B compared to Group C. No significant differences were found in ALT and AST release between Group C and Group A. Livers in Group CC compared to Group BB exhibited significantly lower portal resistance, greater oxygen consumption and bromosulfophthalein excretion into bile and lower lactate dehydrogenase （LDH） release into perfusate. Histological evaluation of tissue sections in Group BB showed parenchymal dystrophy of hepatocytes, while dystrophy ofhepatocytes was absent in Group CC. Livers preserved at 13 ℃ for 24 h exhibited severe ischemic injury Conclusion： These results suggest that the conventional way of liver preservation is not suitable at least for rat livers and that slow cooling of HTK solution during the initial phase of cold storage can improve liver viability during reperfusion.     

一种新型热管充液及其除气方法

热管以为其独特的工作原理,使其成为理想高效的导热元件,在生活和生产中得到广泛应用,从航空航天领域到光电子领域,以及换热器和太阳能领域。但是热管的制造工艺相对严格和困难,因为它独特的设计和相态转换的工作原理,决定了他的特殊性。为了能够提高热管制造工艺,简化工艺过程,本文提出一种热管制造工艺中新的工质填充及其杂气消除方法,该装置主要由灌注系统、抽真空系统、封口系统、加热系统组成。可以完成完整的工质填充和除杂工艺。     

脑缺血再灌注损伤与基因表达调控研究进展

本文对近年来脑缺血再灌注损伤病理机制与基因表达调控研究的新进展做简要综述.     

Inhibition of mitochondria responsible for the anti-apoptotic effects of melatonin during ischemia-reperfusion

Objective： To investigate a possible mechanism responsible for anti-apoptotic effects of melatonin and provide theoretical evidences for clinical therapy. Methods： lschemia-reperfusion mediated neuronal cell injury model was constructed in cerebellar granule neurons （CGNs） by deprivation of glucose, serum and oxygen in media. After ischemia, melatonin was added to the test groups to reach differential concentration during reperfusion. DNA fragmentation, mitochondrial transmembrane potential, mitochondrial cytochrome c release and caspase-3 activity were observed after subjecting cerebellar granule neurons to oxygen-glucose deprivation （OGD）. Results： The results showed that OGD induced typical cell apoptosis change, DNA ladder and apoptosis-related alterations in mitochondrial functions including depression of mitochondrial transmembrane potential （its maximal protection ratio was 73.26%） and release of cytochrome c （its maximal inhibition ratio was 42.52%） and the subsequent activation of caspase-3 （its maximal protection ratio was 59.32%） in cytoplasm. Melatonin reduced DNA damage and inhibited release of mitochondrial cytochrome c and activation of caspase-3. Melatonin can strongly prevent the OGD-induced loss of the mitochondria membrane potential. Conclusion： Our findings suggested that the direct inhibition of mitochondrial pathway might essentially contribute to its anti-apoptotic effects in neuronal ischemia-reperfiusion.