Inhibitive effects of anti-oxidative vitamins on mannitol-induced apoptosis of vascular endothelial cells

Objective： Study blood vessel injury and gene expression indicating vascular endothelial cell apoptosis induced by mannitol with and without administration of anti-oxidative vitamins. Methods： Healthy rabbits were randomly divided into four groups. Mannitol was injected into the vein of the rabbit ear in each animal. Pre-treatment prior to mannitol injection was per- formed with normal saline （group B）, vitamin C （group C） and vitamin E （group D）. Blood vessel injury was assessed under electron and light microscopy. In a second experiment, cell culture specimen of human umbilical vein endothelial cells were treated with mannitol. Pre-treatment was done with normal saline （sample B）, vitamin C （sample C） and vitamin E （sample D）. Total RNA was extracted with the original single step procedure, followed by hybridisation and analysis of gene expression. Results： In the animal experiment, serious blood vessel injury was seen in group A and group B. Group D showed light injury only, and normal tissue without pathological changes was seen in group C. Of all 330 apoptosis-related genes analysed in human cell culture specimen, no significant difference was seen after pre-treatment with normal saline, compared with the gene chip without pre-treatment. On the gene chip pre-treated with vitamin C, 45 apoptosis genes were down-regulated and 34 anti-apoptosis genes were up-regulated. Pre-treatment with vitamin E resulted in the down-regulation of 3 apoptosis genes. Conclusion： Vitamin C can protect vascular endothelial cells from mannitol-induced injury.     

Proliferation of endothelial cell on polytetrafluoroethylene vascular graft materials carried VEGF gene plasmid

Objective： To investigate whether vascular endothelial growth factor （VEGF） gene plasmid carried by polytetrafluoroethylene （PTFE） vascular graft materials could transfect endothelial cells （ECs） and promote their growth. Methods： PTFE vascular graft materials carried with pCDI-hVEGF121, pCDI or pEGFP were incubated in Tris-buffer solution and the values of optical density of 260 nm at different time were plotted, then the DNA controlled release curve was made. ECs derived from human umbilical vein were seeded on the pCDI-hVEGF121/pCDI/pEGFP-PTFE materials or tissue culture plates, ECs numbers were counted and VEGF protein concentrations at different time were measured by enzyme-linked immunoadsorbent assay method. Green fluorescent protein （GFP） expression in ECs on pEGFP-PTFE materials was examined with fluorescence mi- croscopy. Results： The controlled release curve showed that the gene released from PTFE materials was rapid within 8 h, then slowed down and that the gene released continuously even after 72 h. At 24, 72 and 120 h, ECs number and proliferation rate of pCDI-hVEGFI21-PTFE materials were higher than those ofpCDI or pEGFP-PTFE materials （P〈0.05）. VEGF protein concentration of pCDI-hVEGF121-PTFE materials was higher than that of pC DI or pEGFP-PTFE materials at 6, 24, 72 and 120 h （P〈0.01）. GFP expression in ECs on the pEGFP-PTFE materials could be detected by fluorescence microscopy. Conclusion： PTFE graft can be used as a carrier of VEGF gene plasmid, VEGF gene carried by PTFE can transfect ECs and promote ECs growth.     

《儿子与情人》和《母亲大人》中畸形母爱的对比研究

《儿子与情人》和《母亲大人》这两部小说中的母亲都为儿子表现出了极端的畸形的母爱。本文通过比较社会背景,不幸婚姻和自身欲望等因素,分析这两部作品中畸形母爱的深层根源。     

时代的负荷者——再论张爱玲小说的男性形象

张爱玲小说中的男性人物形象基本上可以分为两类,一类是封建没落家族的遗老遗少,另一类是当时社会上的成功人士／生活优裕者。后者没有显赫或富贵的家庭背景,但在社会上拥有一席之地,思想则亦中亦西,在动荡的时代和畸形的文化环境里,他们的精神价值失落,成了都市里的人生失败者。他们的生活和思想特点、他们的得意与失意的悖反都受制于动荡的时代和畸形的文化,他们是“这时代的广大的负荷者”。     

服务与资询

如果您有家教方面的困难,欢迎和我们联系。电话:0551-2609339地址:合肥市长江中路57号《家教世界》杂志社小悠收也可以在我们的博客上留言:blog.sina.com.cn/jiajiaoshijie把握儿童牙齿矫治的最佳时机在儿童的乳牙期(3岁～5岁)和替牙期(6岁～11     

张爱玲小说中的"留洋者"探究

张爱玲在她的小说中塑造了一批身份特殊的"留洋者",他们代表了西方现代文明与中国传统文化的决裂,以拯救者的姿势给女性带来了新的生机与希望,但由于多种合力因素的影响,他们同样沦为特定时代的牺牲品.动荡的社会现实和畸形的家庭环境是张爱玲塑造这批"留洋者"的原因,她企盼以西方现代文明来批判僵化的中国传统文化,为人类寻求解放的新途径.从这点而言.张爱玲无疑继承了鲁迅批判国民性的"五四"文学精神.     

从楚雄市陈家槽子村的拆迁透视楚雄州“扩城战略”

城市化是促进中国经济持续高增长和城乡发展的强大动力,也是解决二元结构矛盾的根本出路.没有城市化的工业化是畸形的、没有后劲的,没有城市化也就没有现代化.加快推进城市化进程是"十一五"期间楚雄州消除城乡差别、实现共同富裕的根本举措,不走城市化道路,要实现真正意义上的现代化就是一句空话.     

浅议教育不公平的原因及对策

一、教育不公平现象及其原因 在高等教育体系中,公然存在教育不公平现象.主要表现在以下三个方面: 1.重点大学和地方院校差距扩大.富有阶层的子弟拼命往重点大学挤,它使原本匮乏且失衡的教育资源更加畸形化.     

五磨脐贴促进腹部手术患者胃肠功能的恢复及对血清血管活性肠肽和降钙素基因相关肽的影响

目的通过观察腹部手术治疗后患者肠麻痹恢复情况,评价五磨脐贴对腹部术后胃肠功能恢复的促进作用;观察腹部手术患者血清中血管活性肠肽和降钙素基因相关肽的变化及五磨脐贴对其影响,探讨五磨脐贴促进腹部手术患者胃肠功能恢复的机理。方法80例患者分为两组,治疗组采用五磨脐贴外敷神阙穴,对照组不使用任何促胃肠动力药,观察患者术后肠鸣音恢复时间及术后肛门排气时间,观察两组血管活性肠肽和降钙素基因相关肽的变化。结果术后肠鸣音恢复时间及术后肛门排气时间组间比较,五磨脐贴治疗组的时间明显短于空白对照组,差异有显著性意义(P<0.01)。治疗前,两组血管活性肠肽和降钙素基因相关肽含量无=明显差异(P>0.05);治疗后,治疗组血管活性肠肽和降钙素基因相关肽较对照组明显降低,差异有显著意义P<0.05);五磨脐贴治疗组治疗前血管活性肠肽和降钙素基因相关肽含量明显高于治疗后,差异有非常显著性意义(P<0.01)。结论五磨脐贴抑制腹部手术患者血管活性肠肽和降钙素基因相关肽的分泌,促进胃肠功能的恢复。     

中西医结合治疗Vogt-小柳原田病92例疗效分析及护理

Vogt-小柳原田(Vogt-Koyanagi-Harada,VKH)病是以双侧肉芽肿性全葡萄膜炎为特征的疾病,常伴有脑膜刺激征、听力障碍、白癜风、毛发变白或脱落。此病又称为“特发性葡萄膜大脑炎”,是国内常见的葡萄膜炎类型之一[1]。患者一般发病急、病情重、治疗时间长,最终视功能受到不同程度