辽东楤木对过度训练大鼠肝脏氧化损伤的预防作用

目的:以大鼠因过度训练而造成的机体损伤为模型,观察辽东楤木提取物对大鼠肝组织脂质过氧化、超氧化物歧化酶(SOD)活性和血清谷丙转胺酶(GPT)活性的影响。方法:将雄性Wistar大鼠随机分4组,即安静对照组(A)、运动组(B)、楤木低剂量加运动组(D)、楤木高剂量加运动组(E)。将大鼠按训练计划进行6周的游泳训练,并对D、E组分别灌服低、高剂量辽东楤木提取物,最后测定MDA含量、GPT活性及SOD活性。结果:过度训练后大鼠肝组织MDA含量和血清GPT活性显著上升(P<0.05),肝组织中SOD活性显著下降(P<0.05),而低剂量楤木提取物可显著抑制这种趋势的发生(P<0.05)。高剂量楤木提取物的作用效果不明显。结论:低剂量辽东楤木提取物对过度训练后大鼠肝脏的氧化损伤具有一定的预防作用。     

A novel way of liver preservation improves rat liver viability upon reperfusion

Background/aim： Currently, the liver is cold-preserved at 0-4 ℃ for experimental and clinical purposes. Here, we investigated whether milder hypothermia during the initial phase of the preservation period was beneficial for liver viability upon reperfusion. Methods： In the first set of experiments, rat livers were preserved either conventionally in clinically used histidine-trypthopan-ketoglutarate （HTK） solution （Group A： 45 min and Group B： 24 h） or by slow cooling HTK solution （from 13 ℃ to 3 ℃） during the initial 45 min of preservation （Group C： 24 h）. In the second set of experiments, additional groups of livers were evaluated： Group BB-preservation according to Group B and Group CC-preservation according to Group C. Further, some livers were preserved at 13 ℃ for 24 h. Livers were then reperfused using a blood-free perfusion model. Results： Bile production was approximately 2-fold greater in Group C compared to Group B. Alanine transaminase （ALT） and aspartate transaminase （AST） release into perfusate were 2-3-fold higher in Group B compared to Group C. No significant differences were found in ALT and AST release between Group C and Group A. Livers in Group CC compared to Group BB exhibited significantly lower portal resistance, greater oxygen consumption and bromosulfophthalein excretion into bile and lower lactate dehydrogenase （LDH） release into perfusate. Histological evaluation of tissue sections in Group BB showed parenchymal dystrophy of hepatocytes, while dystrophy ofhepatocytes was absent in Group CC. Livers preserved at 13 ℃ for 24 h exhibited severe ischemic injury Conclusion： These results suggest that the conventional way of liver preservation is not suitable at least for rat livers and that slow cooling of HTK solution during the initial phase of cold storage can improve liver viability during reperfusion.     

肿瘤基因全排序研究揭示，基因突变可延长胰腺神经内分泌瘤患者生命

据2011年1月22日《参考消息》援引路透社华盛顿2011年1月20日电,巴尔的摩约翰斯·霍普金斯大学的科学家在《科学》杂志撰文公布了他们的这一研究成果。     

组胺对人肝星状细胞合成Ⅰ型胶原的影响

研究组胺对人肝星状细胞(HSC-T6)合成Ⅰ型胶原的影响,探讨肥大细胞(MC)在慢性肝疾病中的作用机理。采用不同浓度(5×10-6、10×10-6、50×10-6、100×10-6mol/L)组胺刺激体外培养的HSC-T6;并设立不同时间点(24、36、48h)分别收集培养上清液,ELISA法测定培养上清液中的Ⅰ型胶原含量。结果表明,组胺能明显促进HSC合成分泌Ⅰ型胶原,且存在一定的时效关系和量效关系,MC可以通过其分泌的生物活性介质(组胺)增强HSC-T6分泌Ⅰ型胶原,而参与慢性肝病发生发展过程。     

胰腺实性假乳头状瘤1例

1 病例介绍 患者女,43岁,主因腹痛伴恶心、呕吐3d于2009-11-25日入我院,既往有多年上腹部不适病史.查体:腹部对称,膨隆,上腹部压痛,以脐周部为著,无肌紧张及反跳痛,剑突下可触及约15cm×10cm大小包块,质中等偏硬,伴触痛,边界尚清,活动度差.     

中药联合人工肝血浆置换治疗肝衰竭疗效观察

结合实际,针对中药联合人工肝血浆置换治疗肝衰竭的方法进行论述。     

安全用好中成药

我们身边有这样的人,一感冒就喝板蓝根,一便秘就吃牛黄解毒片,女性经期不适就吃乌鸡白凤丸……这些耳熟能详的中成药,是许多人眼里不折不扣的"万金油"。可是,与民众对中成药的依赖成反比的是,近年来因中成药使用不当带来的安全问题越来越多,应当按医嘱适量服用,否则对健康有危害。     

夏秋换季暖胃护胃是关键

胃痛为胃病临床常见的一种病证,中医学常常以此命为病名,相当于现代医学之急、慢性胃炎,胃溃疡,胃神经官能症等,均可出现上腹部疼痛,兼有脘腹胀闷,甚则见胀痛攻窜、泛酸烧心,多与情绪恼怒、焦虑、抑郁有关,中医称之为肝胃不和或肝气犯胃。     

初春养肝正当时——春季护肝养生全攻略

春季,人体的生理变化主要是:一是气血活动加强,新陈代谢开始旺盛;二是肝主藏血、肝主疏泄的功能逐渐加强,人的精神活动也开始变得活跃起来。所以,立春后,养生就得养肝。为何肝是疏泄的,还得养肝呢?中医师解释说,古人有句话,"满则损,缺则盈",正如同