种出来的石油

多事石油,可能将不再多事了。欧美强国为了争夺石油屡次发动战争,可能还令你目不暇接;你可能还在为被汽车尾气,刺激得掩面流泪而皱眉;为它将天     

胺醇萃取剂及钯萃合物界面性质研究

研究了胺醇萃取体系的界面吸附和胶团形成热力学,发现萃合物界面吸附能力大于萃取剂,萃合物胶团形成自发进行.通过测定不同稀释剂中萃取剂的界面张力,研究了萃取体系三相形成的原因.     

Study on the expression and mutation of human telomeric repeat binding factor （hTRF1） in 10 malignant hematopoietic cell lines

Objective: Detecting the expression and mutation of human telomeric repeat binding factor (hTRF1) in 10 malignant hematopoietic cell line cells on the base of determining its genomic structure and its four pseudogenes to clarify if h TRF1 mutation is one of the factors of the activation oftelomerase. Methods: hTRF1cDNA sequences were obtained from GenBank, its genome structure and pseudogenes were forecasted by BLAST and other biology information programs and then testified by sequencing.Real-time RT-PCR was used to detect the expression ofhTRF1mRNA in 10 cell line cells, including myelogenous leukemia cell lines K562, HL-60, U-937, NB4, THP-1, HEL and Dami; lymphoblastic leukemia cell lines 6T-CEM, Jurkat and Raji. Telomerase activities of cells were detected by using telomeric repeat amplification (TRAP)-ELISA protocol. PCR and sequencing were used to detect mutation of each exon of h TRF1 in 10 cell line cells. Results: h TRF1 gene, mapped to 8q 13, was divided into 10 exons and spans 38.6 kb. Four processed pseudogenes of hTRF1 located on chromosome 13, 18, 21 and X respectively, was named as ψhTRF1-13, ψhTRF1-18, ψhTRF1-21 and ψhTRF1-X respectively. All cell line cells showed positive telomerase activity. The expression of hTRF1 was significantly lower in malignant hematopoietic cell lines cells (0.0338, 0.0108～0.0749) than in normal mononuclear cells (0.0493, 0.0369～0.128) (P=0.004). But no significant mutation was found in all exons of hTRF1 in 10 cell line cells. Four variants were found in part of intron 1,2 and 8 ofhTRF1. Their infection on gene function is unknown and needs further studies. Conclusion: hTRF1 mutation is probably not one of the main factors for telomerase activation in malignant hematopoietic disease.     

列车之殇谁之过

2008年4月28日凌晨4时41分,由北京开往青岛的T195次旅客列车,在山东胶济铁路周村站至王村站间的一个拐弯处,发生列车脱线事故,机车后第9至17节车厢脱轨。更为不幸的是,尾部车辆脱线后侵入了上行线,与正在上行线由烟台开往徐州的5034次旅客列车发生碰撞,造成5034次列车机车及机车后第1至5节车厢脱轨。这次惨烈的交通事故导致70多人遇难,     

实验小制作两则

制作一:自制滚摆方法一:将两个输液用的葡萄糖瓶子,从瓶口以下6cm处截断,将两瓶的截断处用502胶粘合在一起.从两瓶口的胶塞中心,穿一根长20cm、粗8mm的木棒,用两条弦线紧拴在木棒的两端,再     

新面孔

江宏杰 25岁｜双鱼座｜中国台北右手横板两面反胶 小学3年级,江宏杰因为中午不爱睡觉会眇到其他同学,就被老师叫到外面去玩乒乓球,时间一久,教练觉得他手感不错,就让他每天午休时间过去打球,从此,他就一发不可收拾,爱上了乒乓球这项运动。“我现在是研究生,有时也会出国打球,不过老师很好,为方便我打球,会集中一段时间上课。前两三年,我因为受伤,没怎么出去打比赛,今年我会到德国打联赛。”     

土豆(或面包)中淀粉的检测和观察实验探究

正《普通高中化学课程标准实验教科书》必修二册(人民教育出版社2007年3月第3版)3-4的探究实验2:把碘酒滴到土豆(或面包)后土豆片和面包都呈黑色,观察不出呈蓝色,影响了学生通过此实验得出正确的结论。那么如何解释上述的实验现象和做好此实验呢?笔者对此实验进行了探究,取得了一定的成果。现将对此实验的探究过程介绍给同行做参考。1实验过程及现象(1)实验材料、仪器和试剂     

SMT生产辅料的选择和管理

本文结合SMT产线生产实际需要,阐述了锡膏、助焊剂、贴片胶、洗板水等几种主要SMT生产辅助材料的作用、质量判别与选择方法、日常保管方式及使用要点。     

我心中的好书·中华出版物·《万历十五年》

正推荐理由每一次接触一本好书,都会很激动。比如《万历十五年》,第一本读破了之后,又买了一本。不知是胶订的不牢固,还是我读书时用力过猛。看这本书的时候,会有种舍不得读的感觉。读一读,放一放,比对待美食还要柔软。也因这本书,认识了三联书店。三联书店     

橡皮扫帚

正每次我用完橡皮后,纸上都有很多橡皮屑,不如做个橡皮扫帚吧!我找来铅笔、橡皮、毛笔、剪刀和乳白胶,首先,用铅笔在橡皮上画一个和毛笔头大小一样的圆圈,用剪刀按照圆圈的形状在橡皮上掏个洞,再把毛笔的笔头拆下来,洗干净。然后,我用剪刀把毛笔头尖的地方剪下来,将乳白胶的