补充磷脂对大鼠腓肠肌钝挫伤后腓肠肌氧自由基反应和脂质过氧化反应的影响

为了研究补充磷脂对大鼠腓肠肌钝挫伤恢复效果,采用重物下落装置建立急性骨骼肌钝挫伤模型,损伤后补充磷脂。在损伤后取样测定和计算腓肠肌SOD、CAT、GSH-Px活性及MDA含量变化。与自然愈合组比较结果发现,损伤后第2-10d,补充磷脂能显著提高腓肠肌SOD、GSH-Px活性,显著降低MDA含量,对CAT活性影响未出现显著变化。因此,补充磷脂能明显提高大鼠急性骨骼肌钝挫伤后的腓肠肌中SOD、GSH-Px活性,降低腓肠肌中MDA含量,对于清除大鼠受损腓肠肌氧自由基,加快消除损伤后发生的脂质过氧化反应,降低脂质过氧化水平,提高急性骨骼肌钝挫伤恢复效果具有促进作用。     

Edaravone protects PC12 cells from ischemic-like injury via attenuating the damage to mitochondria

Background： Edaravone had been validated to effectively protect against ischemic injuries. In this study, we investigated the protective effect of edaravone by observing the effects on anti-apoptosis, regulation of Bcl-2/Bax protein expression and recovering from damage to mitochondria after OGD （oxygen-glucose deprivation）-reperfusion. Methods： Viability of PC 12 cells which were injured at different time of OGD injury, was quantified by measuring MTT （2-（4,5-dimethylthia-zol-2-yl）-2,5-diphenyltetrazolium bromide） staining. In addition, PC 12 cells＇ viability was also quantified after their preincubation in different concentration of edaravone for 30 min followed by （OGD）. Furthermore, apoptotic population of PC 12 cells that reinsulted from OGD-reperfusion with or without preincubation with edaravone was determined by flow cytometer analysis, electron microscope and Hoechst/Pl staining. Finally, change of Bcl-2/Bax protein expression was detected by Western blot. Results： （1） The viability of PC12 cells decreased with time （1-12 h） after OGD. We regarded the model of OGD 2 h, then replacing DMEM （Dulbecco＇s Modified Eagle＇s Medium） for another 24 h as an OGD-reperfusion in this research. Furthermore, most PC 12 cells were in the state of apoptosis after OGD-reperfusion. （2） The viability of PC 12 cells preincubated with edaravone at high concentrations （1, 0.1, 0.01 μmol/L） increased significantly with edaravone protecting PC 12 cells from apoptosis after OGD-reperfusion injury. （3） Furthermore, edaravone attenuates the damage of OGD-reperfusion on mitochondria and regulated Bcl-2/Bax protein imbalance expression after OGD-reperfusion. Conclusion： Neuroprotective effects of edaravone on ischemic or other brain injuries may be partly mediated through inhibition of Bcl-2/Bax apoptotic pathways by recovering from the damage of mitochondria.     

胡萝卜整根煮锁住营养又抗癌

将胡萝卜煮熟后再切片,抗癌效果更佳,它所含有的抗癌化合物比先切片的胡萝卜多25%。整个烹饪出来的胡萝卜味道也更好,因为保留了更多糖分,切碎的胡萝卜在烹饪过程中会有更多营养物质流失掉。将胡萝卜整个烹饪或之后再将其切碎,不仅可以锁住更多营养物质,而且口感也更好。提示:胡萝卜放在空气中,营养会通过氧化而流失;放在清水里,营养会直接溶进水中;放在有油的地方,营养也会溶在油脂里而流失。做不到整体烹饪或不切就吃的情况下,也要尽量一次把整个胡萝卜吃完,这样能让营养吸收相对完整。实在吃不完时,能吃多少做多少,剩下的未经烹饪的蔬果则一定要用保鲜膜包起来,放在冰箱里,并尽快食用。     

六（硫氰酸根-N）合铬（Ⅲ）酸钾的合成及电子光谱

合成标题配合物K3[Cr（NCS）6].4H2O并测定其在水溶液中的电子光谱。用RHF-SCF-PM3方法进行了几何优化,研究了配合物基态和激发态组态的电子结构。由ZINDO/S方法的组态相互作用（CI）计算给出了理论光谱,计算结果与实测谱吻合较好。     

铜铬钼／=二氧化硅三元复合加氢催化剂中铜,铬,钼的分析

本文报导了Ｃｕ＝ｅｒ－Ｍｏ／ｓｉｏ２三元复合催化剂中Ｃｕ，Ｃｒ，Ｍｏ的测定方法，此法简便，快速，获得令人满意结果。     

影响动脉粥样硬化的几种生化因素

动脉粥样硬化(Atherosclerosis，AS)是一种以血管内皮细胞(endothelial cell，EC)受损、平滑肌细胞(smooth muscle cell，SMC)增生为主要病理特征的血管疾病．由于AS形成的粥样斑块在血管内易破裂、脱落，形成血栓堵塞心脑血管而造成更为严重的心脑血管疾病，在世界范围内已成为严重危害人民生命健康的疾病之一．文章主要阐述脂质过