增压辅助方法对大鼠骨骼肌HIF-1α表达的影响

目的:通过高原低氧大强度训练与增压辅助方法相结合建立动物模型,探讨高原低氧大强度训练后施加增压辅助方法对大鼠骨骼肌组织HIF-1α表达的影响。方法:wistar大鼠32只随机分为4组:其中A组为自然环境下恢复,B组0.2MPa增压1h恢复,C组0.2MPa增压2h恢复,D组0.3MPa增压2h恢复。4组大鼠在西宁(2260m)经过3天适应性训练和6天正式训练。在最后一次训练结束后24h所有大鼠实施腹腔麻醉取大鼠一侧腓肠肌,运用蛋白质免疫印迹法检测各组HIF-1α蛋白表达量。结果:施加增压辅助方法干预后各恢复组大鼠骨骼肌HIF-1α蛋白表达较自然恢复组呈上升趋势,其中B组增幅较大。结论:通过1周低氧大强度训练后,施加增压辅助方法的实验发现,各增压恢复组大鼠骨骼肌HIF-1α蛋白表达上调,表明高原训练后施加增压辅助方法可能对增强机体低氧耐受能力产生影响,有利于机体运动疲劳的快速恢复以及有效发挥高原训练的优势。     

Age-Related Changes in Soluble Vascular Endothelial Growth Factor Receptor 1 (sVEGFR1) and Receptor 2 (sVEGFR2) in Healthy Japanese Subjects

We measured serum soluble vascular endothelial growth factor receptor 1 (sVEGFR1) and receptor 2 (sVEGFR2) levels in healthy Japanese individuals in order to establish a reference value using a specific ELISA. Significant differences were observed in serum sVEGFR1 and sVEGFR2 levels between children and adults. To demonstrate the usefulness of the reference value for children, we measured serum sVEGFR1 and sVEGFR2 levels in children with diarrhea positive (D+) hemolytic uremic syndrome (HUS) as a preliminary study. Serum sVEGFR2 levels in children with HUS were markedly higher than those in healthy children from the onset of D + HUS. The reference value for healthy children in the present study will allow normal and pathological conditions to be discriminated from each other in future study     

HBME-1、CK19基因在甲状腺结节的表达状态及诊断价值

目的：研究分析HBME-1、CK19基因在甲状腺结节的表达状态及诊断价值。方法：回顾性分析天津肿瘤医院头颈外科1975．1～1998．1月收治的初治甲状腺乳头状癌行甲状腺癌联合根治术的有完整随访资料的93例患者临床、组织病理和免疫组织化学染色资料，与同期收治的15例结节性甲状腺肿、16例甲状腺腺瘤相比较。对常规HE染色证实上述病变的蜡块分别行HBME-1和CK19免疫组织化学染色。结果：93例甲状腺乳头状癌病例中，HBME-1基因阳性表达甲状腺乳头状癌为51例（54．8％），甲状腺良性病变为5例（16．1％）；CK19基因阳性表达甲状腺乳头状癌为39例（41．9％），甲状腺良性病变为4例（12．9％）。两种基因在甲状腺乳头状癌和良性病变中的阳性表达均存在统计学差异。HBME-1、CK19基因表达与甲状腺乳头状癌患者的性别、年龄、肿瘤大小、局部侵犯、颈淋巴结转移无明显相关性。结论：HBME-1、CK19基因在甲状腺乳头状癌中表达增强，在甲状腺结节的鉴别诊断中是有用的辅助手段。CK19在区分甲状腺乳头状癌和乳头状增生中是一个很好的标志物。     

The happy-fish-little-pond effect on enjoyment: Generalizability across multiple domains and countries

Academic enjoyment is an important educational construct given that it benefits students' engagement, persistence, wellbeing, and mental health. In this study, we examine two factors that determine this crucial emotion, namely student- and class-level achievement. Past research has been restricted to single-country or single-domain examinations of secondary school students, limiting generalizability of findings. To bridge this gap, we utilize the Trends in International Mathematics and Science Study and the Progress in International Reading Literacy Study (TIMSS-PIRLS) combined 2011 data (N = 180,084 4th-grade students, 37 countries). Our results provide robust evidence that student-level achievement positively predicts enjoyment in math, science, and reading, while the effects of class-level achievement are negative—the Happy-Fish-Little-Pond Effect. These results showed relative universality across the domains and countries examined.     

Improving Writing Skills of Thai EFL Students by Recognition of and Compensation for Factors of L 1 to L2 Negative Transfer

In this study, we examined the likelihood of a TAME （extended technology acceptance model）, in which the interrelationships among computer self-efficacy, perceived usefulness, intention to use and self-reported use of computer-mediated technology were tested. In addition, the gender- and age-invariant of its causal structure were evaluated. The data were collected from a self-reported questionnaire administered to 477 administrative staff of a public university in Malaysia. The results of structural equation modeling supported the adequacy of TAME. Although the TAME＇s causal structure was applicable to both male and female staff, age group appeared to moderate the structural relationships among the constructs of interest.     

MPG1基因驱动GFP基因在大丽轮枝菌中表达

为了探讨以MPG1基因作为启动子能否驱动GFP基因在大丽轮枝菌中表达,采用根癌农杆菌介导的真菌遗传转化方法,将含有MPG1-eGFP表达盒的pHMG载体转化入棉花大丽轮枝菌T-9中。经抗生素筛选后转化子约80个/106孢子,荧光显微镜观察转化子菌株孢子和菌丝在荧光显微镜下均发出绿色荧光,基因组DNA-PCR检测、GFP蛋白westernblot检测表明,GFP蛋白在大丽轮枝菌转化子中成功表达。结果表明,MPG1基因作为启动子成功驱动GFP基因在大丽轮枝菌中转录表达。     

基于负样本多通道优化SSD网络的钢铁厂提取

准确提取钢铁厂对去产能监测和环境保护具有重要意义。传统的人工目视解译方法效率低、成本高,无法满足开展大区域钢铁厂监测的需求。以深度学习目标检测网络SSD为基础,构建面向遥感影像钢铁厂提取的深度学习目标检测网络,提出maxout模块,将负样本通路优化为多分支结构,突出难分负样本特征并提升网络对无用特征的抵制效果。利用国产GF-1数据对京津冀地区的钢铁厂进行快速自动提取实验。与人工解译的钢铁厂点位数据的对比表明,该目标检测方法的提取精度达到80%以上。     

母语提纲对大一学生二语写作的影响

英语新四级考试作文主要以英语题目加母语提纲为出题方式,大一学生在写作时是否受到母语提纲的负迁移影响？本文将通过对比两个非英语专业的大一班级按照同一题目不同提纲（母语提纲和英语提纲）写的课堂作文,探讨母语提纲对学生作文的影响。