6-甲基-2-（α-羟甲基）-1H-苯并咪唑荧光增敏探针法测定蛋白质

以6-甲基-2-（α-羟甲基）-IH-苯并咪唑（MHMBM）作为荧光探针,利用蛋白质对MHMBM荧光增敏作用,建立了测定人血清总蛋白的新方法。结果表明,在中性水溶液中,MHMBM的荧光强度与牛蛋白（BSA）的浓度呈良好的线性关系,相关系数（r）为0．9995,检出限2．3μg／mL,线性范围4．6—60．0μg／mL。本方法用于测定两种样品溶液中的总蛋白,RSD（n=6）分别为1．7％和1．3％,回收率95．5％和98．6％。     

试论印象主义色彩的统一性

印象主义绘画是欧洲绘画艺术发展的一个重要阶段,也是现实主义艺术向现代主义艺术过渡的一个重要阶段。通过对印象主义绘画的产生、发展、色彩特点及影响进行梳理,可以比较全面认识印象主义绘画的特点对当代艺术发展和创作的促进作用,这对丰富当代的绘画语言有着重要的现实意义。     

高校旅游管理专业“3＋1”教学模式探讨

长期以来,我国高校旅游管理专业本科教学大多实行的是传统的课堂教学模式,其特点是重理论轻实践,导致了学生实践动手能力差、毕业生业内就业率低等一系列问题。随着我国旅游业的快速发展,旅游企业对高素质的专业人才求贤若渴,旅游人才供需矛盾日益凸显。如何才能有效地培养业内需求的高级管理人才,使旅游管理本科毕业生能在业内充分就业,探讨国内旅游管理专业本科教学全面推行＂3＋1＂模式具有十分重要的现实意义。本文在分析高校旅游管理专业本科教学存在问题的基础上,探讨了旅游管理专业本科教学实行＂3＋1＂模式的必要性和可行性,重点提出了实施＂3＋1＂教学模式的相关建议。     

大负荷游泳运动后大鼠额叶神经元凋亡和线粒体形态变化及Drp1、Mfn2表达的研究

目的：：通过观察一次性大负荷游泳运动后线粒体形态结构以及线粒体融合分裂基因及蛋白表达的动态变化,探讨一次性大负荷游泳运动引起大鼠额叶神经元凋亡发生发展的可能机制。方法：72只雄性SD大鼠随机分为对照组(C组,n=18),一次性大负荷游泳运动组(E组,n=54),再根据运动后取材时间的不同将 E组分为：即刻组(E0组,n=18);24h组(E24组,n=18);48h组(E48组,n=18)。 C组常规饲养,E组进行一次性大负荷游泳运动,无负重游泳4小时。用透射电镜观察额叶神经元及神经元线粒体的形态结构;用TUNEL法检测细胞凋亡指数( AI);检测线粒体融合分裂基因Drp1、Mfn2 mRNA转录及蛋白表达水平。结果：检测发现E组大鼠大脑额叶神经元发生了细胞凋亡,E组各组大鼠AI显著性高于C组(P<0.01),E组各时间点AI比较为：E24>E0> E48,各组间差异均具有显著性( P<0.05)。一次性大负荷运动后各时间点Drp1和Mfn2 mRNA转录和蛋白表达均高于C组( P<0.05),其中E24组大鼠大脑额叶Drp1和Mfn2 mRNA转录和蛋白的表达最强(P<0.01);与E24组比较,E48组大鼠大脑额叶Drp1 mRNA转录和蛋白的表达下调显著(P<0.05),而Mfn2 mRNA转录和蛋白的表达下调不明显(P>0.05)。结论：一次性大负荷游泳运动引起大鼠额叶神经元凋亡及神经元线粒体形态结构异常,该异常在运动后48h内呈现动态性变化,机体可能通过调节线粒体融合分裂基因Drp1、Mfn2表达,影响大鼠额叶线粒体的形态结构和功能,从而调控额叶神经元凋亡的发生和发展。     

Effect of high glucose levels on the calcification of vascular smooth muscle cells by inducing osteoblastic differentiation and intracellular calcium deposition via BMP-2/Cbfα-1 pathway

In this paper,we investigate the effect and the possible mechanism of high glucose levels on the calcification of human aortic smooth muscle cells (HASMCs).HASMCs were divided into four groups: normal glucose group (NG),osmolality control group (OC),high glucose group (HG,HASMCs culture medium containing 30 mmol/L glucose),and high glucose plus recombinant human Noggin protein (bone morphogenetic protein-2 (BMP-2) antagonist) group (HN).The mRNA levels and the protein expressions of BMP-2 and core binding factor alpha-1 (Cbfα-1) were measured by real-time quantitative polymerase chain reaction (PCR) and Western blot.After induced by 10 mmol/L β-glycerol phosphoric acid,cells were harvested for assessments of alkaline phosphatase (ALP) activities at Days 1,2,and 3,and intracellular calcium contents at Days 7 and 14,respectively.High glucose levels increased the mRNA levels and the protein expressions of BMP-2 and Cbfα-1 (P0.05).The expression of Cbfα-1 was partially blocked by Noggin protein (P0.05),while BMP-2 was not (P0.05).After being induced by β-glycerol phosphoric acid,high glucose levels increased the ALP activity [(48.63±1.03) vs.(41.42±2.28) U/mg protein,Day 3;P0.05] and the intracellular calcium content [(2.76±0.09) vs.(1.75±0.07) μmol/mg protein,Day 14;P0.05] in a time-dependent manner when compared with the NG group,while the ALP activity could not be blocked by Noggin protein [(48.63±1.03) vs.(47.37±0.97) U/mg protein,Day 3;P0.05].These results show that high glucose levels can evoke the calcification of HASMCs by inducing osteoblastic trans-differentiation and intracellular calcium deposition via the BMP-2/Cbfα-1 pathway,which can be partially blocked by Noggin protein.     

维普数据库Z39.50服务端系统设计方案

介绍了维普数据库Z39.50服务端系统的设计方案，该方案有两条解决途径：基于TCP/IP的Z39.50服务端系统和基于HTTP的SRU服务端系统，笔者将这两种思路做了比较。本系统突破了传统Z39.50协议只运用于对书目数据的提供，而是提供电子期刊数据，这是本方案的特色所在。     

浅谈中国街头篮球的发展现状及其对中国职业篮球发展的影响

运用文献资料和逻辑分析法,研究街头篮球起源及发展的文化背景,分析中国街头篮球的发展历程、现状及发展方向,探讨其对中国职业篮球发展的影响。     

矩阵方程组异类约束解的MCG1-3-5算法

借鉴求线性矩阵方程组同类约束解的MCG算法（修正共轭梯度法）,建立了求多个未知矩阵的线性矩阵方程组的一种异类约束解的MCG1-3-5算法,证明了该算法的收敛性。该算法不仅可以判断矩阵方程组的异类约束解是否存在,而且在有异类约束解,且不考虑舍入误差时,可在有限步计算后求得矩阵方程组的一组异类约束解;选取特殊初始矩阵时,求得矩阵方程组的极小范数异类约束解。同时还能求取指定矩阵在该矩阵方程组异类约束解集合中的最佳逼近。算例表明,该算法有效。     

A theory of instruction for introductory programming skills

ABSTRACT

Background and Context: Current introductory instruction fails to identify, structure, and sequence the many skills involved in programming.

Objective: We proposed a theory which identifies four distinct skills that novices learn incrementally. These skills are tracing, writing syntax, comprehending templates (reusable abstractions of programming knowledge), and writing code with templates. We theorized that explicit instruction of these skills decreases cognitive demand.

Method: We conducted an exploratory mixed-methods study and compared students’ exercise completion rates, error rates, ability to explain code, and engagement when learning to program. We compared material that reflects this theory to more traditional material that does not distinguish between skills.

Findings: Teaching skills incrementally resulted in improved completion rate on practice exercises, and decreased error rate and improved understanding of the post-test.

Implications: By structuring programming skills such that they can be taught explicitly and incrementally, we can inform instructional design and improve future research on understanding how novice programmers develop understanding.     

广义(3+1)维KPB方程的对称约化、精确解和守恒律

运用李对称方法得到了(3+1)维Kadomtsev Petviashvili Boussinesq(KPB)方程的对称和约化方程。进而,利用齐次平衡原理和椭圆函数方法得到了方程的精确解。最后,给出了该方程的守恒律。