A study of the relationship between expression level of TRF1 protein and telomerase activity in human acute leukemia

INTRODUCTION Telomeres are distinctive DNA-protein struc-tures that cap the ends of linear chromosomes.It is very important to keep the chromosomes stabilization.Telomerase activity is closely linked to attainment of cellular immortality,a step in carcinogenesis,while lack of such activity contributes to cellular senes-cence.Telomerase is activated in more than85%ofmalignant tumors(Hiayma et al.,1997).Human te-lomeric repeat binding factor1(TRF1)is a telomere associated with proteins a…     

手法及快针治疗急性腰肌扭伤60例

文章系统介绍了手法、快针、治疗急性腰肌扭伤的有效性,探讨治疗本病的最佳疗法。方法:临床60例急性腰肌扭伤患者1～2天,将特定的手法,快针直刺哈是穴,治疗1～2次。结果:治愈率100%。结论:这种治疗是我多年的临床实践经验,效果确切,可靠、安全方便,立见功效。     

Concurrent pulmonary thrombosis with systemic embolism: a case report

Based on different mechanisms of blood coagulation, coexistence of venous thromboembolism and arterial thrombosis in a single individual is extremely rare in clinical practice. Both antiplatelet and anticoagulation therapy should be adopted for patients with arteriovenous embolism. Balancing the risk of ischemia and hemorrhage is especially challenging in these patients in order to achieve an optimal clinical benefit. We report on a 55-year-old female with acute pulmonary embolism (PE), subsequently diagnosed as having acute myocardial infarction (AMI) and a cerebral infarction. Examinations had been carried out, excluding potential arteriovenous shunts, cancer, antiphospholipid syndrome and other common hypercoagulable states. A combination of an anticoagulant drug (rivaroxaban, an Xa inhibitor) and an antiplatelet agent (clopidogrel, an ADP receptor inhibitor) was prescribed with a β-blocker and atorvastatin. The embolus was gradually shrunk during the next 10 months, and then it turned back into expanding. During the 16 months’ follow-up, an aneurysm of left ventricular apex was found through an echocardiogram and an angiotensin-converting enzyme inhibitor was administered. We conclude that combined anticoagulation and antiplatelet therapy significantly relieved the symptoms and improved the prognosis in patients suffering from arteriovenous embolism without any major clinical bleeding events.     

Effect of Palrnatine on lipopolysaccharide-induced acute lung injury by inhibiting activation of the Akt/NF‍-κB pathway

Inflammation plays an important role in the development of acute lung injury (ALI). Severe pulmonary inflammation can cause acute respiratory distress syndrome (ARDS) or even death. Expression of proinflammatory interleukin-‍1β (IL-‍1β) and inducible nitric oxide synthase (iNOS) in the process of pulmonary inflammation will further exacerbate the severity of ALI. The purpose of this study was to explore the effect of Palrnatine (Pa) on lipopolysaccharide (LPS)-induced mouse ALI and its underlying mechanism. Pa, a natural product, has a wide range of pharmacological activities with the potential to protect against lung injury. Western blotting and quantitative real-time polymerase chain reaction (qRT-PCR) assays were performed to detect the expression and translation of inflammatory genes and proteins in vitro and in vivo. Immunoprecipitation was used to detect the degree of P65 translocation into the nucleus. We also used molecular modeling to further clarify the mechanism of action. The results showed that Pa pretreatment could significantly inhibit the expression and secretion of the inflammatory cytokine IL-1β, and significantly reduce the protein level of the proinflammatory protease iNOS, in both in vivo and in vitro models induced by LPS. Further mechanism studies showed that Pa could significantly inhibit the activation of the protein kinase B (Akt)/nuclear factor-κB (NF-κB) signaling pathway in the LPS-induced ALI mode and in LPS-induced RAW264.7 cells. Through molecular dynamics simulation, we observed that Pa was bound to the catalytic pocket of Akt and effectively inhibited the biological activity of Akt. These results indicated that Pa significantly relieves LPS-induced ALI by activating the Akt/NF-κB signaling pathway.     

运动性横纹肌溶解征并发急性肾衰竭1例报道

运动性横纹肌溶解征并发急性肾衰竭(ARF)是一类较少见的急性肾衰竭。文章通过对剧烈运动致急性肾衰竭1例的发生和临床诊治的报道,旨在加强对本病的认识与预防。     

支链氨基酸与运动的关系

本文在广泛查阅相关文献的基础上,就支链氨基酸与能量代谢、蛋白质代谢,支链氨基酸与运动性中枢疲劳、外周疲劳以及支链氨基酸的毒性等方面加以综述并进行可行性分析论证。结果表明:支链氨基酸与人体运动能力关系密切,能够为长时间的耐力运动提供能量储备,促进肌肉蛋白质的合成,而且可以调节引发运动性疲劳的外周机制,推迟运动性疲劳的出现,运动后补充支链氨基酸还可以促进运动性疲劳的恢复。但至于支链氨基酸对蛋白质分解代谢的影响及其抗中枢疲劳效应,在长期训练中补充BCAA是否有减少和修复肌肉损伤的能力,是否存在性别差异以及服用大量BCAA是否对大脑和肌肉产生消极作用,导致肠胃功能的紊乱则还有待进一步的研究与探索。     

体育专业学生神经类型与急性运动损伤的关系

探讨神经类型与急性损伤的关系，为教学、训练以及运动员选材提供依据。采用80．8神经量表和运动损伤调查表，对256名体育专业大学生进行测试。结果显示稳定型、中间型、弱型的大学生有着更高的损伤率，男、女生的急性损伤率在灵活、易扰、中间类型上存在差异。     

清咽汤治疗急性扁桃体炎45例临床观察 --附西药治疗组41例对照观察

本文就清咽汤治疗急性扁桃体炎45例观察，与西医对照组比较，说明清咽汤能明显缩短病程，具有较好清泄热毒作用。     

急性肾功能衰竭家兔心肌损伤的体液机制

目的：观察急性肾功能衰竭（ARF）家兔心肌匀浆自由基、一氧化氮的变化，探讨心肌损伤的体液机制。方法：60只家兔均分为四组（n=15）。ARF模型1组：皮下注射1％HgCl2（1．3ml／kg．bw）；ARF模型2组：肌肉注射50％甘油（10ml／kg．bw）；以等量生理盐水代替HgCl2和甘油作为对照1、2组。24h后，所有动物颈总动脉放血备检，并选择固定位置。制备10％心肌匀浆。经Aeroset型全自动生化分析仪测定血清反映肾功能的生化指标。检测心肌匀浆丙二醛（MDA）、超氧化物歧化酶（SOD）、一氧化氮（NO）及其合酶（NOS）的变化。结果：与相应对照组比较，ARF模型1、2组心匀浆SOD活性下降、MDA含量升高（P〈0．05），ARF模型1、2组心匀浆NO含量增强、NOS及iNOS活性增强（P〈0．05～0．01）。结论：ARF家兔心肌损伤的机制与自由基损伤及NO升高有关。