Three-dimensional tracking of GLUT4 vesicles in TIRF microscopy

TIRF microscopy has provided a means to view mobile granules within 100 nm in size in two dimensions. However quantitative analysis of the position and motion of those granules requires an appropriate tracking method. In this paper, we present a new tracking algorithm combined with the unique features of TIRF. Firstly a fluorescence correction procedure was processed to solve the problem of fluorescence bleaching over time. Mobile granules were then segmented from a time-lapse image stack by an adaptive background subtraction method. Kalman filter was introduced to estimate and track the granules that allowed reducing searching range and hence greater reliability in tracking process. After the tracked granules were located in x-y plane, the z-position was indirectly inferred from the changes in their intensities. In the experiments the algorithm was applied in tracking GLUT4 vesicles in living adipose cells. The results indicate that the algorithm has achieved robust estimation and tracking of the vesicles in three dimensions.     

SOFC temperature evaluation based on an adaptive fuzzy controller

The operating temperature of a solid oxide fuel cell （SOFC） stack is a very important parameter to be controlled, which impacts the performance of the SOFC due to thermal cycling. In this paper, an adaptive fuzzy control method based on an affine nonlinear temperature model is developed to control the temperature of the SOFC within a specified range. Fuzzy logic systems are used to approximate nonlinear functions in the SOFC system and an adaptive technique is employed to construct the controller. Compared with the traditional fuzzy and proportion-integral-derivative （PID） control, the simulation results show that the designed adaptive fuzzy control method performed much better. So it is feasible to build an adaptive fuzzy controller for temperature control of the SOFC.     

网络环境下有效学习行为特征及影响因素研究

在网络环境下,学习者由于高度自由同时又缺乏有效的监管,学习很容易受到外界事件的干扰,并不能完全保证学习者的学习行为是有效的。为保证网络环境下学习的质量与绩效,有必要对网络环境下的有效学习行为进行深入研究。文章在“有效学习”定义基础上,提出网络环境下有效学习行为的概念及其特征,并详细讨论了网络环境下有效学习行为的主要影响因素,最后从中得到提高网络环境下学习行为有效性的启示:在课前,教师要做好学前分析,检查学习者是否已有本次学习必要的基础知识储备,并帮助学习者明确自己的学习目标,给学习者提供必要的学习环境;在课中,教师要注重学习者信息技术应用能力的培养,同时给学习者施加一定的外部动机,并结合具体情况,给予学习者有效的引导;在课后,教师需要及时对这节课进行反思总结。     

噪声主动控制的进展及发展趋势

对噪声主动控制技术领域的进展及发展趋势进行综述.包括消声原理、消声器结构和算法等方面的研究成果,分析该技术存在的缺陷,对今后的主要发展方向作出预测.     

(Re)framing a philosophical and epistemological framework for teaching and learning in STEM: Emerging pedagogies for complexity

Today’s learners are engaging in study where access to knowledge is easier than it ever has been in human history. Rapid advancement of technology and the increasing ease with which communication and interaction can occur has dramatically changed the landscape in which teachers of science, technology, engineering and mathematics (STEM) operate. The contemporary skills that students are required to possess include inter alia problem solving, creativity, teamwork abilities, communication skills and emotional intelligence. Despite the universal acceptance of their importance, these skills are commonly cited as underdeveloped and in addition, are still accompanied by outmoded ‘traditional’ forms of teaching and assessment. While the approaches of twentieth-century education were successful in developing knowledge stores, the ubiquity of access to knowledge—coupled with the constantly changing nature of the world today—requires alternative conceptions of teaching and learning. This article focuses primarily on an exploration of learning metaphors and teaching with the overall lens of creating self-regulated and furthermore, self-determined learners. The article begins with an exploration of learning in STEM education and a critique of the pedagogical perspective, discussing why this epistemology may be insufficient for contemporary STEM learning. The article then considers an alternative and potentially more contemporary notion; the emergent pedagogic space. The article presents a theoretical model to conceptualise learning in STEM education, with the goal of informing both practice and research. The realisation of this proposed emergent pedagogical space is explored through an applied case study from a design and technology context.     

Actuator fault diagnosis for a class of bilinear systems with uncertainty

In this paper, the actuator fault diagnosis problem for a class of bilinear systems with uncertainty is discussed. The system is transformed into two different subsystems. One is not affected by actuator fault, so an adaptive observer can be designed such that, under certain conditions, the observer error dynamics is stable. The other whose states can be measured is affected by the faults. The observation scheme is then used for model-based fault diagnosis. Finally, an example of a semiactive suspension system is used to illustrate the applicability of the proposed method.     

微博社区成员参与的心理影响因素实证分析

利用逐步多元回归分析法,探明并实证分析了微博社区成员参与行为的心理影响因素。研究发现,成员的自我呈现、感知方便性和感知可信性对他们的参与行为存在显著影响。并且,自我认同和社会强化能显著地影响成员投入到微博社区中的努力。理论层面上,研究结论为下一步参与行为的心理作用路径研究和群体心理与行为研究提供前提;实践层面上,对微博服务提供者经营和管理微博社区以及政府相关部门管理微博具有现实意义。     

From scraps to reams to mobile screens? An update on printing services in academic libraries

As a follow-up to their 2001/2002 study on printing services in academic libraries, the authors explore how the provision of printing services has changed over the last twelve years. This article explores how libraries are choosing to fund printing services as well as how technology, such as scanning and mobile devices, has changed the way printing services are provided. The results of the 2013 survey find a greater percentage of libraries are using a fee-based system for printing, and, while some technology is providing greater data and control for printing services, other technological advances are having unintended consequences for libraries’ printing infrastructure.     

Supporting adaptive learning pathways through the use of learning analytics: developments,challenges and future opportunities

Learning Analytics (LA) and adaptive learning are inextricably linked since they both foster technology-supported learner-centred education. This study identifies developments focusing on their interplay and emphasises insufficiently investigated directions which display a higher innovation potential. Twenty-one peer-reviewed studies are identified via a systematic review and classified based on the dimensions of a proposed framework. The findings of the review suggest that interesting work has been carried out during the last years on the topic. We conclude with a need for more research on the topic in specific learning domains and settings. Recommendations include: a clear strategy for adaptation augmented by LA, the combination of on-task with pre-task measures, and the combination of system-controlled adaptation with user-controlled adaptation. Future trends include the emergence of constructivist-collaborative environments that provide insightful models of complex student behaviour.     

自然美的衰退与人的审美行为的成熟

随着现代文明的发展,人们对自然的审美行为愈益成熟,即对自然进行审美时运用太多的分析技巧,合理化意识支配着他;这足以导致自然美的衰退。要想使自然美有更多的呈现就必须恢复审美行为的单纯性,借助情感、联想、想象同自然进行富有生气的沟通,并对自然有一种描写与抒情的欲望。在当代生态环境面临恶化的条件下,这种面对自然的单纯性的审美行为具有积极的意义。