促进知识转化的B2C电子商务实验设计

实验教学能促进知识的转化和创新,在电子商务高等教育中占有重要的地位。本文针对B2C电子商务的教学,提出了一种B2C电子商务模拟实验的设计方案,围绕如何促进知识转化和创新介绍了该模拟实验的设计。     

计算机模拟镍铝合金中的马氏体相变

计算机分子动力学方法在对材料的结构和性质的研究中正扮演着一个重要的角色．镍铝合金长期以来，在材料的强度、轻重、高温以及结构方面是一个重要的候选对象．镍铝合金中的马氏体相变是一种特殊的并且在应用领域有重要价值的相变．因此用计算机分子动力学方法来模拟这一相变过程。     

一种能实现文件权限控制的文件上传下载系统

对上传下载的目标文件夹创建目录索引,再为索引设置不同的文件操作权限等属性,实现了B/S模式下教师能控制学生上传下载权限的文件上传下载系统。上传下载时,由索引的链接转向来定位目标文件夹位置,借Asp网页Session保存的索引ID来传递操作权限等属性,根据权限属性在文件操作网页上显示不同的文件操作功能,实现了不同索引不同权限的控制。     

荧光PCR基因分析仪的快速并行温度控制

提出一种模仿人工经验的智能升降温控制系统,成功地应用于荧光PCR基因分析仪,同时也给出DS18B20温度传感器的详细用法及程序。     

《计算机信息技术基础》Web课程的设计与实现技术

文章从《计算机信息技术基础》Web课程的基本要求出发，阐述了课程设计的主要过程和应该遵循的一些基本原则，同时用实例详尽地阐明了开发一个Web课程的设计方法和实现技术。     

Hydrogen sulfide from a NaHS source attenuates dextran sulfate sodium (DSS)-induced inflammation via inhibiting nuclear factor-κB

This study investigated the alleviating effects of hydrogen sulfide (H₂S), derived from sodium hydrosulfide (NaHS), on inflammation induced by dextran sulfate sodium (DSS) in both in vivo and in vitro models. We found that NaHS injection markedly decreased rectal bleeding, diarrhea, and histological injury in DSS-challenged mice. NaHS (20 µmol/L) reversed DSS-induced inhibition in cell viability in Caco-2 cells and alleviated pro-inflammation cytokine expression in vivo and in vitro, indicating an anti-inflammatory function for H₂S. It was also found that H₂S may regulate cytokine expression by inhibiting the nuclear factor-κB (NF-κB) signaling pathway. In conclusion, our results demonstrated that H₂S alleviated DSS-induced inflammation in vivo and in vitro and that the signal mechanism might be associated with the NF-κB signaling pathway.     

郑州大学基础课实验教学中心信息平台建设与应用

为了适应对基础课实验教学中心实行网络化、智能化管理的需求，构建了基于客户端／服务器（C／S）和浏览器／服务器（B／S）两种模式的“郑州大学基础课实验教学中心信息平台”。文章论述了信息平台的建设目标与设计思路，并对信息平台的系统结构以及各级用户的权限、功能、操作等做了详细说明。     

Basic fibroblast growth factor alleviates brain injury following global ischemia reperfusion in rabbits

The aim of this study was to explore the protective effect of basic fibroblast growth factor (bFGF) on brain injury following global ischemia reperfusion and its mechanisms. Brain injury following global ischemia was induced by four vessels occlusion and systemic hypotension. Twenty-four rabbits were randomized into three groups: group A, only dissection of vessels; group B, intravenous infusion of normal saline after reperfusion for 6 h; group C, 30 μg/kg bFGF injected intravenously at the onset of reperfusion, then infused with 10 μg/(kg·h) for 6 h. Serum neuron specific enolase (NSE), S-100B, tumor necrosis factor-α(TNF-α), interleukin-1 (IL-1), interleukin-8 (IL-8) were measured before ischemia, 30 min after ischemia, 0.5, 1, 3, 6 h after reperfusion. Brain water content was determined and cerebral histopathological damages were compared. NSE and S-100B were increased 1 h after reperfusion and reached their peaks 6 h after reperfusion, but were much higher in group B than those in group C 3, 6 h after reperfusion. In groups B and C, TNF-a was increased after ischemia and IL-1 and IL-8 were increased significantly 0.5 h after reperfusion, then reached their peaks 6 h, 3 h, 6 h after reperfusion respectively. TNF-a and IL-8 at the time points of 1 h and 3 h and IL-1 at 3 h and 6 h in group C were correspondingly lower than those in group B. These indices in group A were nearly unchanged. There were less severe cerebral histopathological damages in group C compared with group B, but no difference in brain water content. It could be concluded that bFGF alleviates brain injury following global ischemia and reperfusion by down-regulating expression of inflammatory factors and inhibiting their activities.     

2500例门诊患者肝功能及血清HBV感染指标检测结果分析

目的 :了解乙肝患者肝功能、乙肝五项及HBVDNA的变化及其相关性。方法 :收集门诊患者血清标本 ,同时检测肝功能、HBVELISA和HBVDNA三个不同水平。结果 :在收集的2 5 0 0例标本中 ,肝功能异常率为 6 2 .8% ,HBs -Ag阳性率为 71.2 % ,HBVDNA定量阳性率为 90 .4 %。当病毒拷贝数在 10 5～ 10 6之间时 ,肝功能异常率为 4 .2 85 % ,HBV拷贝数增加肝功能也不同程度加重 ;HBs -Ag阴性的标本中 ,FQ -PCR检出率为 30 .5 6 % ;单独HBs -Ab阳性的FQ -PCR检测的阳性率为 2 2 .35 %。结论 :HBVDNA的FQ -PCR是目前检测乙肝最为敏感的方法     

Intrathymic inoculation of donor liver specific antigen alleviates rejection of liver allotransplantation

Use and effects of liver specific antigen in orthotopic liver transplantations were researched in this study. Group I: syngeneic control (Wistar-to-Wistar); Group II: acute rejection (SD-to-Wistar); Group III: Thymic inoculation of SD rat LSA day 7 before transplantation. The observation of common situation and survival time, rejection grades, NF-κB activity of splenocytes and IL-2mRNA expression of grafted liver were used to analyze acute rejection severity and immune state of animals in different groups. The common situation of group I was very well after transplantation and no signs of rejection were found. Recipients of group II lost body weight progressively All dead within day 9 to day 13 posttransplantation; median survival time was 10.7 ±0.51 days. It was an optimal acute rejection control. As for group III, 5 out of 6 recipients survived for a long time and common situation was remarkably better than that of group II. Its rejection grades were significantly lower than that of group II(P<0.05). NF-κB activity was only detected in group I at day 5 and day 7 after transplantation, whereas high activity of NF-κB was detected at all time points in groupII and the low NF-κB activity detected in group III was significantly lower than that of group II (P<0.05). No IL-2mRNA expression was detected at any time point in group I, whereas high level expression was detected at all time points in group II and the low level expression only detected at day 3 in group III was significantly lower than that of group II (P<0.05). Conclusion: LSA is an important transplantation antigen which is involved directly in the immunorejection of liver transplantation. We report here for the first time that intrathymic inoculation of LSA can alleviate the rejection of liver allotransplantation; and that grafts can survive for a long time thereby, thus leading to a novel way to achieve liver transplantation immunotolerance. Project (NO. 011106206) supported by the Science and Technology Bureau of Zhejiang Province Foundation