生物学年龄与运动员生长发育鉴定

运动员生长发育鉴定在运动员科学选材中十分重要,而生物学年龄的鉴定又是运动员生长发育鉴定的关键.本文综合文献与选材工作经验,详细介绍了几种常用的生物学年龄:骨龄与第二性征是目前运动员生长发育鉴定中常用的方法;牙龄在运动员选材中应用较少,主要受技术和方法等条件的限制,需进一步探索,增加应用的可行性;端粒DNA长度、线粒体DNA损伤和DNA甲基化水平等分子生物学方法推断年龄是比较新的方法,在运动员科学选材领域尚未得到验证与应用.最后本文展望了运动员生长发育鉴定中生物学年龄发展的前景.     

稻草栽培草菇高产技术探讨

将稻草经过加工处理 ,调整传统配方 ,添加适量废棉等辅助原料 ,用以栽培草菇 ,进行严格控温管理 ,草菇生物转化率达 35% ,比对照组高 1 2— 1 5% ,达增产目的     

物种和植被资源信息系统的建设及展望

着重阐述了生物物种和植被资源信息系统的基本组成和基础数据库，并简要介绍了生境评价系统的研究成果和植被资源-气候环境的空间模拟结果。     

立足地区特色培养模式下的毕业论文实践

毕业论文是高等院校实践教学中的重要环节。我们对毕业论文实践教学进行了初步的探索,旨在找出适合地方高等院校的办学模式下的生物类专业毕业论文实践教学,以提高本科生培养质量,开辟一条具有地区特色的实践之路。     

Hyperhydration using different hydration agents does not affect the haematological markers of the athlete biological passport in euhydrated volunteers

ABSTRACT

Athlete Biological Passport (ABP) is an indirect approach, implemented by WADA, aimed at detecting blood manipulation based on abnormal changes in haematological markers. Cases report the use of hyperhydration as masking method during anti-doping urine sample collection which could potentially mask suspicious fluctuations on ABP profiles. This study investigated the hyperhydration effect on haemoglobin concentration, reticulocyte percentage and OFF-hr score (an algorithm based on haemoglobin concentration and reticulocyte percentage), with and without recombinant human erythropoietin (rHuEPO) administration. A five-week clinical study performed; Baseline and rHuEPO Phase. Water and a sports drink were used as hyperhydration agents. To examine the hyperhydration effect on the normal ABP profile per volunteer, hyperhydration was implemented at 0, 24 and 48 hours during the baseline. During the rHuEPO phase, volunteers received Epoetin beta (3000 IU) with hyperhydration to be implemented at 0, 24 and 48 hours after drug administration. Blood and urine samples were collected and analysed according to WADA guidelines. No significant effect on ABP markers was observed due to hyperhydration at any time during the study. Pre- and post-hyperhydration data were not statistically different compared to individual baseline data. In conclusion, hyperhydration does not affect the ABP haematological markers under the examined conditions.     

Production of carotenoid sarcinaxanthin by Kocuria palustris isolated from Northeastern Brazil Caatinga soil and their antioxidant and photoprotective activities

BackgroundThis study aimed to produce carotenoids of two bacterial strains obtained and isolated from Caatinga soil in Northeastern Brazil and to evaluate their antioxidant and photoprotective activities. The morphological identification of bacteria was performed by Gram staining and molecularly confirmed through the 16S rRNA gene. The production of carotenoids was performed on two 2³ factorial designs to analyze the influence of independent variables (temperature range, luminosity, agitation, spiral presence, and bacterial isolate type) for maximum carotenoid yield. The selected condition has been transferred to a bioreactor (10L). The identification of carotenoids was performed by liquid chromatography (HPLC) and mass spectrometry (LC-MS). Antioxidant activity was determined by inhibiting the β-carotene/linoleic acid system and the effectiveness as sunscreen was measured through its sun protection factor (SPF).ResultsThe results revealed that the isolates FT-7.22 and FT-5.12 were identified as Kocuria palustris; producers of a rare C50 carotenoid sarcinaxanthin. This is the first report on the production of carotenoids by this species from the Caatinga Domain. The pigment that was obtained from the Tryptic Soy Broth (TSB) medium in the best conditions of the factorial designs (increased agitation, aeration, and light exposure) exhibited a significant increase in the carotenoid production. The isolated FT-7.22 reached a higher sarcinaxanthin concentration (112,480 μg/L), and it exhibited promising antioxidant (76.53 ± 0.09%) and photoprotective activities (SPF = 9.36 ± 0.52).ConclusionsThis study demonstrated the ability of K. palustris to produce carotenoid sarcinaxanthin with antioxidant and photoprotective activities so that it can be applied in cosmetic formulations.How to cite: Mendes-Silva TCD, Vidal EE, de Souza RFR, et al. Production of carotenoid sarcinaxanthin by Kocuria palustris isolated from Northeastern Brazil Caatinga soil and their antioxidant and photoprotective activities. Electron J Biotechnol 2021;53. https://doi.org/10.1016/j.ejbt.2021.05.004     

动物的生物节律及其机制

本文阐述了动物的生物节律的概念，影响动物的生物节律的因素，并对生物节律的机制进行论述。     

对训练分期问题研究的认识

随着竞技体育产业化和商业化的发展。比赛增多，训练界对传统的训练分期理论有不同的看法和争论，本文在对文献资料研究基础上，阐述了有关训练分期研究中造成困惑的问题     

我国生物多样性保护的主要进展及工作建议

生物多样性是人类赖以生存的物质基础。我国高度重视生物多样性保护工作,积极采取了一系列卓有成效的行动和举措,促进了我国生物多样性的保护和物种恢复。然而,受人类活动影响,全球生物多样性面临的形势仍然严峻,需要各国同心协力,加强全球生物多样性保护。文章系统整理了近些年我国在生物多样性保护方面的举措,以及中国科学院在生物多样性研究领域的若干工作,并对我国今后生物多样性保护工作提出建议。     

纤维素生物活性材料的种类及应用

纤维素的功能化一直是人们研究的热点,近年来又涌现出一批以纤维素为基准的具有生物性的活性材料。文章主要论述了以纤维素为基准的细菌纤维素、复合材料、纤维素硫酸钠材料等具有生物活性材料的种类与应用。