彭桂萼边疆史地研究的学术思想特点

彭桂萼的边疆论著,是当前研究西南边疆有价值的历史资料之一,也是云南的重要文献之一。他的研究克服了前代学者研究滇西南囿于书本的局限,以实地履勘与文献考证相结合,著述真实可信。综观他的边疆论著,具有明显的爱国爱乡的思想倾向;实地调查,创新体例;认清边疆形势,加强边防建设;风土人情,记载周详的特点。     

In vitro study on ribozyme against hepatitis C virus

Objective: To determine the effective nucleotide sites of ribozymes against HCV, and obtain a highly effective, nontoxic and inexpensive antisense ribozyme specific for HCV. Methods: Two effective ribozymes, targeted to HCV 5’NC region and C region, were designed and synthesized. Eukaryotic expression vectors, pSV2-gpt. CD-SR (, containing either HCRZNC or HCRZC were constructued and transfected into MT-2 cells, which had been infected by HCV. Quantitative PCR and hydridization were used to determine the effect of inhibition of HCV by ribozymes. Results: HCRZNC and HCRZC suppressed the replication of HCV by 54.7% and 62.1%, respectively. Furthermore, when both ribozymes were cotransfected into cells, they suppressed replication by 78.8%. Conclusion: Two specific antisense ribozymes have strong inhibitory effects on the replication of HCV in cultured cells, and have better effect when used together.     

水稻胚乳细胞发育与结实特性

文章对水稻胚乳细胞发育过程、淀粉体和蛋白体结构特征与结实特性、养分输入胚乳细胞的途径、ATPase活性分布与水稻充实的关系、水稻强弱势粒胚乳细胞发育与其结实特性进行分析.提出进一步研究胚乳细胞发育及其超微结构与籽粒生理活性的关系,可从深入开展亚显微水平下强弱势粒背部维管束结构、ATPase的活性分布、差异表达基因对结实特性的影响等方面展开研究.     

对《鄂豫边区党委关于减租情况致华中局、中央报告》一文的时间考证

现存《鄂豫边区党委关于减租情况致华中局、中央报告》这一历史文件，其成文年代不详。本文根据该文件所涉及内容的时间性和文件中有关具时间性含义的语句，结合若干外证，考订出该文件的成文时间为1942年4月11日。     

Allograftic bone marrow-derived mesenchymal stem cells transplanted into heart infarcted model of rabbit to renovate infarcted heart

Objective: To investigate the directed transplantation of allograftic bone marrow-derived mesenchymal stem cells (MSCs) in myocardial infarcted (MI) model rabbits. Materials and Methods: Rabbits were divided into 3 groups, heart infarcted model with MSCs transplanted treatment (MSCs group, n=12), heart infarcted model with PBS injection (control group, n=20), sham operation with PBS injection (sham group, n=l 7). MSCs labelled by BrdUrd were injected into the MI area of the MSCs group. The same volume of PBS was injected into the MI area of the control group and sham group. The mortality, LVIDd, LVIDs and LVEF Of the two groups were compared 4 weeks later. Tropomyosin inhibitory component (Tn I) and BrdUrd immunohistochemistry identified the engrafted cells 4 weeks after transplantation. Result: The mortality of the MSCs group was 16.7% (2/12), and remarkably lower than the control group's mortality [35% (7/20) (P＜0.05)].Among the animals that survived for 4 weeks, the LVIDd and LVIDs of the MSCs group after operation were 1.17±0.21 cm and 0.74±0.13 cm, and remarkably lower than those of the model group, which were 1.64±0.14 cm and 1.19±0.12 cm (P＜0.05); the LVEF of the MSCs group after operation was 63±6%, and remarkably higher than that of the model group,which was 53±6% (P＜0.05). Among the 10 cases of animals that survived for 4 weeks in the MSCs group, in 8 cases (80%),the transplanted cells survived in the non MI, MI region and its periphery, and even farther away; part of them differentiated into cardiomyocytes; in 7 cases (70%), the transplanted cells participated in the formation of blood vessel tissue in the MI region. Conclusion: Transplanted allograftic MSCs can survive and differentiate into cardiomyocytes, form the blood vessels in the MI region. MSCs transplantation could improve the heart function after MI.     

Study of Resistin gene expression in peripheral blood mononuclear cell and its gene polymorphism in a small range population

INTRODUCTION Recently discovered as an endocrine organ, adi-pose tissue can secrete many cytokines such as Re-sistin, adiponectin and free fatty acids, and receives much attention in the study of insulin resistance and type 2 diabetes mellitus (T2DM). As a peptide hor-mone secreted by adipocyte, Resistin is considered to be the linkage between obesity and insulin resistance (Steppan et al., 2001). Most studies on Resistin in-vestigate adipose tissue. In this experiment we de-tected t…     

明朗的画面 温柔的悲剧——论沈从文《边城》童话式的美

沈从文是一个热情的理想主义者,《边城》是他充满深情唱出的一曲生命的理想之歌,是他出于＂怀旧＂的主观诗情并把它凝结成的＂诗＂,是沈从文梦里故园的产物。他写湘西,实际上是通过故园风物的眷念来补偿人生的失落,以慰藉他那魂灵深处的创伤。主要从画中人、画中物、画作者、画外意三个方面来解开这个梦,为读者打开神秘之窗。梦是美丽的,而＂美丽的东西总使人忧愁＂,为了淡化这宿命的悲剧,沈从文以＂善＂与＂美＂进行了巧妙的阐释,从而圆了这个作于都市的＂乡下人＂的梦。     

晶体硅太阳电池接触电阻测量

金属电极与硅的接触电阻是太阳电池中串联电阻的一部分,是影响太阳电池填充因子和短路电流进而影响光电转换效率的重要因素之一,通过了解接触电阻的大小,可以判断太阳电池电极的优劣。接触电阻的测量,较为困难,难以精确,笔者给出了一种基于PN Vinod三点测试方法的改进方法及测试实例。     

集训对舞龙队女大学生血常规及内皮祖细胞的影响

目的:分析舞龙运动对女大学生血常规及内皮祖细胞含量的影响。方法:选取2017级舞龙队女大学生10人为训练组,2017级普通女大学生10人作为对照组。舞龙队女大学生备战比赛进行连续4周,每周3次,每次90min的赛前集训,普通女大学生除日常活动外,没有额外进行任何其他运动队的集训。集训结束后,在受试者都处于空腹状态下对两组受试者进行静脉采血并进行血常规、早期内皮祖细胞和晚期内皮祖细胞的检测。所得数据进行统计学分析。结果:参加舞龙集训的女大学生的血常规指标与普通女大学生的血常规指标无显著差异,早期EPCs和晚期EPCs无显著性差异,但在数值上显示出不同的趋势。结论:参加舞龙集训的女大学生身体处于健康状态。参加舞龙集训有可能增强运动员血管的自我修复能力。     

神经营养素家族类细胞因子对神经干细胞分化影响的研究现状

神经干细胞（NSCs）是具有高度自我更新能力并能分化为神经元、星形胶质细胞和少突胶质细胞的神经前体细胞,具有广阔的临床应用前景。神经营养素家族对神经干细胞的分化有一定影响。其包括：神经生长因子（NGF）、脑源性神经生长因子（BDNF）、神经营养素-3（NT-3）、神经营养素-4/5（NT-4/5）以及神经营养素-6（NT-6）。本文就目前神经营养素家族对NSCs分化的影响的研究现状进行综述。