大负荷游泳运动后大鼠额叶神经元凋亡和线粒体形态变化及Drp1、Mfn2表达的研究

目的：：通过观察一次性大负荷游泳运动后线粒体形态结构以及线粒体融合分裂基因及蛋白表达的动态变化,探讨一次性大负荷游泳运动引起大鼠额叶神经元凋亡发生发展的可能机制。方法：72只雄性SD大鼠随机分为对照组(C组,n=18),一次性大负荷游泳运动组(E组,n=54),再根据运动后取材时间的不同将 E组分为：即刻组(E0组,n=18);24h组(E24组,n=18);48h组(E48组,n=18)。 C组常规饲养,E组进行一次性大负荷游泳运动,无负重游泳4小时。用透射电镜观察额叶神经元及神经元线粒体的形态结构;用TUNEL法检测细胞凋亡指数( AI);检测线粒体融合分裂基因Drp1、Mfn2 mRNA转录及蛋白表达水平。结果：检测发现E组大鼠大脑额叶神经元发生了细胞凋亡,E组各组大鼠AI显著性高于C组(P<0.01),E组各时间点AI比较为：E24>E0> E48,各组间差异均具有显著性( P<0.05)。一次性大负荷运动后各时间点Drp1和Mfn2 mRNA转录和蛋白表达均高于C组( P<0.05),其中E24组大鼠大脑额叶Drp1和Mfn2 mRNA转录和蛋白的表达最强(P<0.01);与E24组比较,E48组大鼠大脑额叶Drp1 mRNA转录和蛋白的表达下调显著(P<0.05),而Mfn2 mRNA转录和蛋白的表达下调不明显(P>0.05)。结论：一次性大负荷游泳运动引起大鼠额叶神经元凋亡及神经元线粒体形态结构异常,该异常在运动后48h内呈现动态性变化,机体可能通过调节线粒体融合分裂基因Drp1、Mfn2表达,影响大鼠额叶线粒体的形态结构和功能,从而调控额叶神经元凋亡的发生和发展。     

The Impact of an Erasmus Placement in Students’ Perception of their Intercultural Communicative Competence

Pre- and post-questionnaires answered by UK Nottingham Trent University (NTU) and Spain’s University of Salamanca (USAL) students on their placement abroad support a comparative analysis of its impact on their intercultural communicative competence, comprising the awareness, attitude, skills and knowledge dimensions. NTU students started their placements with better results in them all. Yet, while both groups completed their stay with a similarly increased awareness, the knowledge and skills of the USAL group matched the results of NTU students and, whereas the scores of the attitude dimension towards the host country of NTU students did not increase significantly, those of USAL students decreased.     

非金融机构开展互联网金融的现况、影响及监管

2005年开始,国内兴起非金融机构经营互联网金融业务的热潮。非金融机构提供在线互联网金融服务,经营业务主要包括“支付”与“投、融资”两大类,其形态主要有四：第三方支付、基金销售、P2P网络借贷,以及众筹。非金融机构经营互联网金融,虽可加快国内金融业转型的步伐,并满足中小企业及个人无法自银行取得资金的投、融资需求,但也因发展过速,监管不足,致使网络借贷倒闭事件频传,不利于金融稳定等诸多负面效应。为此,需要主管机关研究制定与互联网金融相关的法律,加强监管,并准许这些非金融机构转型为正规商业银行。     

Utility of P-SEP,sTREM-1 and suPAR as Novel Sepsis Biomarkers in SARS-CoV-2 Infection

The coronavirus disease 2019 is a highly contagious viral infection caused by SARS-CoV-2 virus, member of coronaviridae family. It causes life threatening complications due to complexity and rapid onset course of the disease. Early identification of high-risk patients who require close monitoring and aggressive treatment remains challengeable till date. Novel biomarkers which help to identify high risk patients at the early stage is high priority. Objective of this review to find utility of P-SEP, sTREM-1 and suPAR for diagnosis, risk stratification and prognosis of SARS-CoV-2 infected cases. Soluble receptors like, P-SEP, sTREM-1 and suPAR have been involved in immune regulation in SARS-CoV-2 infection and elevate more in severe cases. A comprehensive research of databases like PubMed, EMBASE, CNKI and Web of Science was performed for relevant studies. A total of nine out of fifteen research literature in initial screening were included for this review. Interestingly all studies have reported high levels of P-SEP, sTREM-1 and suPAR in SARS-CoV-2 infected cases and the biomarkers positively correlated with severity of infection. This implies that P-SEP, sTREM-1 and suPAR can be implemented as surrogate marker in blood profile for early diagnosis, risk stratification and prognosis in SARS-CoV-2 for better management in Indian population at the current situation.     

下一代数字图书馆系统体系架构的发展动向

文章介绍了国外近年来下一代数字图书馆系统体系架构研究的新动向,着重分析了SOA、网格和P2P技术在数字图书馆领域内应用的研究及所开发的原型系统.     

中国经济空间格局演变及其CO2排放效应

改革开放以来,中国经历了由东部率先发展到区域协调发展的区域战略调整,中国经济空间格局发生较大改变.探究CO2排放量对经济空间格局演变的响应,对中国尽早实现CO2排放达峰目标、确保可持续发展具有重要意义.本文在刻画1985-2017年中国经济空间格局演变特征的基础上,基于LMDI因素分解方法分析了中国CO2排放的驱动因素...     

ONP脱墨浆的可漂性研究

ONP脱墨浆往往需要进一步漂白。对5种用不同脱墨剂处理的ONP脱墨浆进行了H2O2漂白实验,结果表明不同脱墨剂处理的ONP白度增加幅度不相同,各脱墨浆的可漂性并不相同。实际使用ONP脱墨浆时需考虑其漂后白度增幅潜力。     

NiCo_2O_4纳米结构阵列的水热合成与氧析出特性研究

钴酸镍纳米材料具有良好的电催化氧析出反应(Oxygen Evolution Reaction,OER)活性,是一种很有发展潜力的非贵金属电催化材料。文章以碳纤维纸为基底,采用水热合成的方法对纳米结构钴酸镍的生长进行了系统的研究,分别考察了生长温度、前驱体浓度和配比、生长时间对产物的形貌、尺寸、物相结构、OER电化学性能的影响,通过对比分析后发现在生长温度100℃、生长时间5h、镍钴比为2:1时所获得的多孔片状钴酸镍纳米结构具有最佳的OER特性。     

一种基于模糊语言TOPSIS的应急决策方法

在应急决策过程中,决策信息通常具有犹豫性、模糊性和非对称性,且决策者呈现出直观、快速的语言决策偏好。提出一种基于模糊语言TOPSIS的应急决策方法,使用犹豫模糊语言术语集和二元语义表达式对高度不确定环境下的语言进行处理,提高对犹豫模糊语言信息的提取能力,生成易于理解的语言评估结果,从而辅助决策者进行快速、准确的决策判断。案例证实该方法的可行性与有效性,为解决应急决策问题提供了一种新方法。     

Responses to oral glucose challenge differ by physical activity volume and intensity: A pilot study

BackgroundOne-hour postprandial hyperglycemia is associated with increased risk of type 2 diabetes and cardiovascular disease. Physical activity (PA) has short-term beneficial effects on post-meal glucose response. This study compared the oral glucose tolerance test results of 3 groups of people with habitually different levels of PA.MethodsThirty-one adults without diabetes (age 25.9 ± 6.6 years; body mass index 23.8 ± 3.8 kg/m²; mean ± SD) were recruited and divided into 3 groups based on self-reported PA volume and intensity: low activity < 30 min/day of moderate-intensity activity (n = 11), moderately active ≥ 30 min/day of moderate-intensity PA (n = 10), and very active ≥ 60 min/day of PA at high intensity (n = 10). Participants completed an oral glucose tolerance test (50 g glucose) with capillary blood samples obtained at baseline, 15 min, 30 min, 45 min, 60 min, 90 min, and 120 min post-ingestion.ResultsThere were no significant differences between groups for age or body fat percentage or glycated hemoglobin (p > 0.05). The groups were significantly different in terms of baseline glucose level (p = 0.003) and, marginally, for gender (p = 0.053) and BMI (p = 0.050). There was a statistically significant effect of PA on the 1-h postprandial glucose results (p = 0.029), with differences between very active and low activity groups (p = 0.008) but not between the moderately active and low activity groups (p = 0.360), even when baseline glucose level and gender differences were accounted for. For incremental area under the curve there was no significant effect of activity group once gender and body fat percentage had been accounted for (p = 0.401). Those in the low activity group took 15 min longer to reach peak glucose level than those in the very active group (p = 0.012).ConclusionThe results suggest that high levels of PA have a beneficial effect on postprandial blood glucose profiles when compared to low and moderate levels of activity.