国际化视角——Cell Research办刊经验点滴

Cell Research自创刊起始终坚持国际化办刊理念和视角,发展到现在已具有较好的国际化基础,具有一定的国际影响,已被国际同行认可.本文从国际合作和引进高端编辑人才两方面介绍了Cell Research近年来办刊的重要举措,希望能对期刊出版同行和各级领导指导创办我国的国际性期刊有所借鉴.     

基于知识生成的工作场所学习

工作场所学习作为一种有别于传统"学校本位教育"的学习方式,发生在工作场所之中,其内涵经历了知识技能观、交互观、过程观和混合观四个阶段的发展演化。伴随新媒体技术在工作场所中的应用与普及,工作场所学习的内涵发生了新的变化:工作学习一体化、集体知识群建共享、工作学习内容生成而非预设等。知识生成视角下的工作场所学习是工作场所学习的高级形式,它的提出是以知识生成隐喻为基础,强调集体知识库的生成、虚实结合的协作交流与分享、学习者之间的交流与互动。学习元平台作为一种能够支持协同创建、内容生成以及语义关联的知识社区,可以支持虚实结合的工作场所学习方式,实现知识与技能获得、参与工作任务、知识系统化生成三个阶段的学习。     

拳卷地钱总黄酮提取物抗乙型肝炎病毒体外实验研究

目的:通过体外实验,探讨拳卷地钱总黄酮提取物抗乙肝病毒的作用。方法:将拳卷地钱总黄酮提取物作用于HBV-DNA转染细胞系2.2.15细胞,通过检测细胞培养液中HBsAg、HBeAg的变化来评价拳卷地钱总黄酮提取物抗乙肝病毒的效果及其可能的药理活性部位。结果:当拳卷地钱总黄酮提取物的最大无毒浓度为31.3μg·mL-1,对2.2.15细胞HBeAg、HBsAg分泌抑制率分别为51.56%、39.69%;治疗指数(TI)>2。结论:在2.2.15细胞无毒浓度下,拳卷地钱总黄酮对2.2.15细胞HBsAg、HBeAg分泌具有比较好的抑制作用;且可以有效阻止HBV-DNA表达和复制。提示:拳卷地钱总黄酮提取物在体外有一定的抗乙肝病毒作用。     

Construction of a eukaryotic expression vector pEGFP-C1-BMP-2 and its effect on cell migration

Objective  

Bone morphogenetic proteins (BMPs) are known to play an important role in bone and cartilage development. Recent research has shown that BMPs may induce tumorigenesis and promote tumor to spread, but the molecular mechanisms have not been elucidated. The aim of the present study was to investigate the regulatory function of BMP-2 in the migration of COS-7 cells and the underlying mechanisms.     

运动对自然杀伤细胞免疫的影响

自然杀伤细胞(NK)以其独特的细胞毒活性在机体对抗各种病毒的第一道防线中起着重要作用,其数量和活性是反映机体免疫系统功能的重要指标。中小强度有规律的运动可以提高NK细胞数量及NK细胞的毒活性,增强机体的免疫功能;而大强度力竭运动则降低NK细胞的数量和毒活性,减弱机体的免疫功能。     

Correlation of matrix metalloproteinase-2, −9, tissue inhibitor-1 of matrix metalloproteinase and CD44 variant 6 in head and neck cancer metastasis

This study aimed to explore the molecular mechanism in tumor invasion and metastasis. The expression of matrix metalloproteinase-2, −9 (MMP-2, MMP-9), tissue inhibitor-1 of matrix metalloproteinase (TIMP-1), cell adhesion molecule 44 variant 6 (CD44v6), HER2/neu and p53 was investigated in 154 patients with head and neck squamous cell carcinoma (SCC) by ABC and ImmunoMax immunohistochemical method. Their clinical relevance and correlation were analysed. The expression of MMP-2, MMP-9, TIMP-1, CD44v6, HER2/neu and p53 was found in cancer cells in 87.01%, 85.71%, 68.18%, 98.05%, 55.19% and 50.65% cases respectively. Linear regression and correlation analysis revealed that there was close positive relationship (P<0.05) between the expression of MMP-2 and MMP-9, TIMP-1 and CD44v6, HER2/neu and MMP-9, MMP-2 and p53. Up-regulation of MMP-2 was accompanied by advanced T stage (P<0.01). There was also a trend of MMP-2 expression being related with tumor metastasis. Increased expression of HER2/neu was found in patients with tumor recurrence (P<0.05). The expression of TIMP-1 was higher in laryngeal cancer than that in pharyngeal cancer, and higher in keratinizing and non-keratinizing SCC than that in basaloid SCC (P<0.05). These findings suggested that MMP-2 and MMP-9, HER2/neu and MMP-9, MMP-2 and p53 had a coordinate function in aggression of tumor; that MMP-2 had a more important function than MMP-9 in tumor invasion and metastasis; and that HER2/neu might serve as a biomarker for poor prognosis in HNSCC. Project supported by Lübeck Medical University, Germany     

细胞生物学双语教学面临的问题及解决方法

文章详细论述了细胞生物学双语教学的意义、面临的问题及解决的对策,实施双语教学的具体方法、思路,重点讲述了课堂的教学组织以及课余的准备工作,为开展细胞生物学双语教学提供了理论基础.     

CDMA网络定位方式及其应用

本文从无线定位技术的原理出发详细介绍了CDMA网络中的定位三种方式以及定位技术的简单应用     

Review:Acute lung injury/acute respiratory distress syndrome (ALI/ARDS): the mechanism,present strategies and future perspectives of therapies

INTRODUCTION OF ACUTE LUNG IN- JURY/ACUTE RESPIRATORY DISTRESS SYN- DROME (ALI/ARDS) Acute lung injury (ALI) and its more severe form, the acute respiratory distress syndrome (ARDS) are syndromes of acute respiratory failure, which was defined by radiological (bilateral lung field infiltrates) and physiological (the ratio of arterial oxygen pres- sure and the inspiratory oxygen concentration, Pa O 2 /Fi O2≤300 mmHg for ALI and ≤200 mmHg for ARDS) criteria in…