细胞生物学教学改革与实践

对细胞生物学课程的教学内容、教学方法和手段、课程考核方法等进行了合理的调整和优化改革,以适应新形式下素质教育的需要,全面提高教学质量,努力培养高素质的专业人才.     

康脑液对皮质区干细胞因子基因表达的影响

目的:在康脑液方剂干预下观察皮质区内源性神经细胞干细胞因子(SCF)mRNA在大鼠脑缺血再灌注损伤后不同时间的表达情况。方法:成年SD大鼠,以线栓法建立大脑中动脉缺血再灌注模型,随机分为模型组、药物干预组和假手术组。原位杂交技术检测脑缺血1.5h再灌注1～14d后,脑皮质区SCFmRNA表达情况。结果:脑缺血再灌注后,药物干预组、模型组SCFmRNA的表达在皮质区均明显高于假手术组,于第7天达高峰,第14天下降。结论:药物干预后脑缺血再灌注脑皮质区SCFmRNA表达在不同时间点与模型组具备相同的表达规律,两组SCFmRNA的表达无显著性差异。     

Enhanced expression of HMG-Y proteins in proliferating tissues

The high mobility group (HMG) proteins I and Y are well characterised non-histone chromosomal proteins which bind to A-T rich regions of DNA and regulate gene expression and/or DNA replication. A correlation has been demonstrated between the increased expression of HMG-Y proteins and malignancy. However, it is not known whether the expression of HMGs particularly, the Y group, is a function of proliferation rate. In the present study, we have used normal tissues of calf testes, thymus and liver. The results show distinctly high expression of HMG-Y proteins in testes than in thymus and the expression was practically undetectable in liver. The results suggest that even in normal tissues there is a direct correlation between the proliferation rate and the expression of the HMG-Y proteins, which can partly explain its increased expression in cancer.     

Particle Swarm Optimization based predictive control of Proton Exchange Membrane Fuel Cell （PEMFC）

INTRODUCTION Fuel cells have attracted more attention in the last few years due to scarcity of the world energy source. The Proton Exchange Membrane Fuel Cell (PEMFC) is the focus of current development efforts because it is capable of higher power density and faster start-up than other fuel cells (Zhang et al., 2004). Research emphasis is on high power density with adequate energy conversion efficiency. PEMFC performance is related to many factors, among which electrolyte membrane …     

Science Letters:Cellular growth under hydrostatic pressure using bovine aortic EC-SMC co-cultured ePTFE vascular graft

High blood pressure (hypertension) is implicated in the development of atherosclerosis. Blood vessels are constantly subjected to stretch due to blood pressure and changes in stretch usually instigate adaptive vascular remodeling, including abnormal growth and proliferation of vascular smooth muscle cells (VSMCs) as well as     

Preparation and in vitro studies of microencapsulated cells releasing human tissue inhibitor of metalloproteinase-2

Objective: To prepare microencapsulated cells releasing human tissue inhibitor of metalloproteinase-2 (TIMP-2), and investigate their biological characteristics in vitro. Methods: Chinese hamster ovary (CHO) cells were stably transfected with a human TIMP-2 expression vector, encapsulated in barium alginate microcapsules and cultured in vitro. Morphological appearance of the microcapsules was observed under a light microscope. Cell viability was assessed using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Enzyme linked immunosorbent assay (ELISA) and reverse zymography were used to confirm the release of biologically active TIMP-2 from the microcapsules. Cryopreservation study of the microencapsulated cells was carried out using dimethyl sulfoxide (DMSO) as preservative agent. Results: The microcapsules appeared like a sphere with diameter of 300–600 μm. The surface of the capsule wall was clearly smooth. The microencapsulated cells survived well and kept proliferating over the 6 weeks observed. No significant difference in TIMP-2 secretion was found between encapsulated and unencapsulated cells. Reverse zymography confirmed the bioactivity of MMP (matrix metalloproteinase) inhibition of TIMP-2. The cryopreservation process did not damage the microcapsule morphology nor the viability of the cells inside. Conclusion: Microencapsulated engineered CHO cells survive at least 6 weeks after preparation in vitro, and secrete bioactive TIMP-2 freely from the microcapsules.     

无脑儿心脏的组织学观察与比较

目的:探讨无脑儿与正常胎儿心脏结构的差异,为畸形胎儿的心脏发育研究提供形态学方面的资料。方法:用H.E染色,通过光镜观察无脑儿及正常胎儿的心脏结构。结果:无脑儿心脏的蒲肯野细胞数量明显低于同龄正常胎儿。结论:无脑儿神经系统发育异常影响其心脏内蒲肯野细胞的发育。     

Al3+对玉米根芽生长影响的研究

近年来，土壤中可溶性铝增加，对作物生长产生重要影响，本文研究了酸性条件下不同浓度Al^3＋对玉米根芽生长的影响。结果表明，Al对玉米根芽的生长有显著的抑制作用．且随着浓度的升高抑制作用显著加强，但时于根的生长Al^3＋浓度有一个相对促进其生长的浓度（0．5mg／L）；同时，高浓度的Al^3＋能诱导玉米根尖染色体发生多种类型畸变。     

COMPUTER SIMULATION FOR ELECTRICALLY DRIVEN MOTION OF FLUID

In this paper,an equation system of electrohydrodynamics(EHD)based onthe fluid motion equations is discussed.Emphasis is put on the effects of electrical forceand surface tension upon the fluid motion.The Marker and Cell method is used to set up acomputational simulation program of electrically driven motion of fluid.With the help ofthe program,a cylindrical fluid under the influences of electrical field and surface tensionhas been calculated.The result is in good agreement with the experimental observation.