Clones identification of Sequoia sempervirens (D.Don) Endl.in Chile by using PCR-RAPDs technique

A protocol of polymerase chain reaction-random amplified polymorphic DNAs (PCR-RAPDs) was established to analyse the gene diversity and genotype identification for clones of Sequoia sempervirens (D.Don) Endl.in Chile.Ten (out of 34) clones from introduction trial located in Voipir-Viilarrica,Chile,were studied.The PCR-RAPDs technique and a modified hexadecyltrimethylammonium bromide (CTAB) protocol were used for genomic DNA extraction.The PCR tests were carried out employing 10-mer random primers.The amplification products were detected by electrophoresis in agarose gels.Forty nine polymorphic bands were obtained with the selected primers (BG04,BF07,BF 12,BF13,and BF 14) and were ordered according to their molecular size.The genetic similarity between samples was calculated by the Jaccard index and a dendrogram was constructed using a cluster analysis of unweighted pair group method using arithmetic averages (UPGMA).Of the primers tested,5 (out of 60) RAPD primers were selected for their reproducibility and high polymorphism.A total of 49 polymorphic RAPD bands were detected out of 252 bands.The genetic similarity analysis demonstrates an extensive genetic variability between the tested clones and the dendrogram depicts the genetic relationships among the clones,suggesting a geographic relationship.The results indicate that the RAPD markers permitted the identification of the assayed clones,although they are derived from the same geographic origin.     

智利玛丽娜铜矿床的地质特征及找矿标志

玛丽娜铜矿区位于智利中生代陆相-湖泊相拉伸拗陷带,形成于中酸性火山岩中,多条平行或网脉状矿体赋存在下白垩统拉斯契加斯(Las Chilcas)组岩层,受断裂控制,矿体切穿多种赋矿围岩,矿石矿物呈细粒浸染状和脉状,矿种以铜为主,伴生银。矿物分带从浅到深呈现出辉铜矿-斑铜矿-黄铁矿的分带特征。近矿围岩蚀变以泥化-青磐岩化-绢云母化为主,深部见钾长石化蚀变。通过对成矿地质条件和矿化特征研究认为玛丽娜铜矿床上部为曼陀型矿床,深部为斑岩型铜矿床。本文在总结项目组勘查和综合研究的基础上,对矿区火山岩的地球物理特征进行研究,探讨其地质意义。     

¡No lucro! … from protest catch cry to presidential policy: is this the beginning of the end of the Chilean neoliberal model of education?

This paper surveys the delivery of school education in Chile over the last half-century. It focuses on evidence provided by recent academic studies on the impact of neoliberal education policies introduced by the military regime in the 1980s, and continued by successive democratic governments. It offers insights into recent popular critiques of the neoliberal model, as reported in the Chilean press, and reflected by significant popular support for student protest movements demanding the end of for-profit schools, and the revitalisation of the public school system – encapsulated in the protesters’ catch cry of ¡No lucro! It considers possible consequences of the adoption, as policy, of that catch cry, by the recently elected Bachelet government.     

Getting there and staying in: first-generation indigenous students’ educational pathways into Chilean higher education

Drawing on a total of 26 life history interviews with indigenous students in higher education, the article examines the role of activating valued resources and personal strategies to navigate unequal pathways into higher education. In Chile, the historical inequalities for indigenous people’s access to higher education are beginning to be reverted, but these changes misrecognize ongoing disadvantages in regard to experiencing university life. Selection choices regarding the institution and course are based on restricted information sources and prior knowledge, whilst the capital required to succeed is heavily biased toward higher socioeconomic backgrounds. This notwithstanding, students’ transitions into higher education are marked by spontaneous adaptations to work routines, managing crises, and the activation of other resources for ‘staying in’. Emphasis is placed on the resilience expressed by these young people, as the first-generation from their families to access higher education, to negotiate cumulative disadvantages from low-quality educational establishments and poverty.     

The ethics of competition: accountability policy enactment in Chilean schools’ everyday life

ABSTRACT

Public policies have a moral order, an ethical horizon. They offer a vocabulary of imagined micro-policies. Using the case of Chile, this paper examines the ways in which accountability policies are reworked within schools and how they affect actors’ subjectivities. It adds new findings to the existing body of research on school accountability policies, offering in-depth evidence based on the case of Chile, which has a high-stakes testing model and a widespread competitive voucher system. The research is based on case studies of ten public and private subsidised schools, framed by a sociological perspective of policy enactment theory. The research findings show the ways that accountability policies are recreated, expanded, and intensified at the local level, permeating an ethic of competition. The analysis focuses on three qualitative trends: school actors’ sense-making of test scores and labels; zones of safety and risk for teachers under an accountability regime; and the emergence of a sticky web of persuasion, surveillance, and coercion among school members in order to improve performance. The practices examined are not understood as ‘secondary effects’ or an ‘implementation problem’, as if they occur unconnected from the policy rationale. The outcomes are consistent with the policy itself in interaction with school life.     

智利职工养老制度改革评析

智利是世界上实现职工养老制度改革和转轨的成功典范。本文对智利职工养老制度改革和转轨的关键环节:个人帐户资本积累制、民营管理公司投资营运养老金基金和政府承担隐性养老金债务等方面,进行评析,为中国职工养老制度改革和转轨提供借鉴。     

What drives the formation of ‘valuable’ university–industry linkages?: Insights from the wine industry

Most of the literature on university–industry (U–I) linkages assumes that these linkages are beneficial per se. We question this assumption, suggesting that not all such linkages are equally helpful. In this paper, we explore the factors driving the formation of ‘valuable U–I linkages’, conceived as those linkages between universities and firms that have a higher potential to diffuse knowledge to other firms in their regional economy. Our empirical strategy combines case-study methodology with econometric techniques using data from two wine clusters in Chile and in Italy. The firm's knowledge base is found to be a key driver of ‘valuable’ U–I linkages. We conclude that selectivity should be encouraged among policy makers endeavouring to promote U–I linkages.