Preparation of high-affinity rabbit monoclonal antibodies for ciprofloxacin and development of an indirect competitive ELISA for residues in milk

A convenient competitive enzyme-linked immunosorbent assay (ELISA) for ciprofloxacin (CPFX) was developed by using rabbit monoclonal antibodies (RabMAbs) against a hapten-protein conjugate of CPFX-bovine serum albumin (BSA). The indirect competitive ELISA of CPFX had a concentration at 50% inhibition (IC₅₀) of 1.47 ng/ml and a limit of detection (LOD) of 0.095 ng/ml. The mAb exhibited some cross-reactivity, however, not so high with enrofloxacin (28.8%), ofloxacin (13.1%), norfloxacin (11.0%), fleroxacin (22.6%), and pefloxacin (20.4%). And it showed almost no cross-reactivity with other antibiotics or sulfonamides evaluated in this study. The competitive ELISA kit developed here could be used as a screening tool to detect and control illegal addition of CPFX in food products. This kit had been applied to milk detection and the recovery rates from samples spiked by CPFX were in a range of 63.02%–84.60%, with coefficients of variation of less than 12.2%.     

创造图书馆服务竞争中的“长尾效应”

随着三网融合以及3G时代的到来,各大厂商纷纷推出了各自的电子阅读终端,这种不受时间、空间限制的阅读方式深受读者喜爱。与之相比,受各种因素的制约图书馆将面临失去用户的威胁。本文针对图书馆这种困境,利用长尾效应提出了图书馆发展新用户、稳住老用户的几点策略。     

近四年我国竞争情报的研究热点及现状分析

文章通过选取收录在中文期刊全文数据库近四年全部期刊作为切入点,以关键词为研究对象,采用词频统计和内容分析法,利用EXCEL为数据统计工具对竞争情报研究领域的主要研究方向进行关键词的变化状况研究和现状分析,从关键词的角度对竞争情报研究中的领域及其热点进行了探讨及分析。参考文献8。     

中国式摔跤“竞技运动教材化”在大学教学中的思考研究

本文主要通过文献资料法、逻辑分析法等方法对我国中国式摔跤"竞技运动教材化"的发展现状及该运动发展中存在的问题进行研究分析。研究表明:中国式摔跤"竞技运动教材化"具有无限可能,可以通过提炼专项运动的核心元素,并着重排除过度专业化的素材,使课程保留中国式摔跤运动的半专业性,使大众了解这项运动的价值,并鼓励更多的人参加这项运动。     

六安市第三产业内部结构和竞争力分析

利用2004—2007年六安第三产业统计数据与钱纳里标准比较,表明六安第三产业还存在一定差距,选取具有代表性部门——运输邮电仓储、批发零售、餐饮住宿、金融、房地产、旅游等——作为研究对象,采用偏离份额分析法,详细分析了六安第三产业的内部各部门结构和竞争力。分析得出结论,六安第三产业部门中,只有房地产增长速度和竞争力较强,而金融业发展速度慢,无竞争力,其他部门发展速度较慢,有一定的竞争优势。     

构建企业战略联盟

构建企业战略联盟是加强中国现代企业国际竞争力的重要方式。战略联盟的核心思想即为“竞合”,改变传统的以竞争对手消失为目标的对抗性竞争,为合作而竞争,靠合作来竞争以寻求企业竞争优势。     

入世过渡期内国有商业银行的对策研究

我国商业银行正处于入世过渡期，既存在许多优势，又要面对许多困难，深化商业银行改革，从根本上完善运行机制和管理机制，优化内部控制机制，坚持自身特色服务，积极发展中间业务，提高竞争力。     

我国竞争情报的发展策略

如何发展竞争情报事业,是目前我国环境下一个亟待深入探讨的重要课题。本文从六个方面提出了我国竞争情报发展的具体策略。     

Do private schools improve public school quality or increase stratification?

Utilizing a 4-year longitudinal dataset (2007−08 to 2010−11) of 212 public secondary schools from two districts in Nepal, I conduct panel regression analyses to determine the association between public school high stakes examination outcomes and measures of private sector competition. I find that there does not seem to be a significant positive or negative association between public school outcomes and the extent of private competition in recent years. However, there is evidence to suggest that the gap between public and private school outcomes is higher in localities with higher private sector growth, providing suggestive evidence of stratification.     

论健美操运动员的身体素质训练

良好的身体素质训练是竞技健美操训练的基础,文章针对青少年的关力量、耐力、速度、柔韧、灵敏的训练方法进行了分析与总结,旨在提高竞技健美操身体素质训练的科学性。