盐浓度、酸碱度及DNA浓度对增色效应实验的影响

研究了DNA在不同浓度，不同pH值和不同氯化钠溶液中紫外吸光度的变化，为设计DNA的增色效应实验提供了依据。     

微课在数学分析教学中的应用

随着无线网络的全覆盖和手机等移动产品的普及,基于“微课”的学习越来越普遍,“微课”必将成为一种新的适应时代发展的教学模式和学习方法。基于微课小而精、制作简单、形式新颖、学习的时间和地点比较自由的特点,本文探索利用微课辅助教学,拓展教学新方法。     

空间高精度定位姿柔顺工作平台输入输出模型的研究

A precise micro-location compliant stage with elastics-supported system is enmployed for investigations.The general motion of stage supported by elastic structure is described.The analyses of forward displecement and inverse displacement of stage are formulated.The study of input-output behavior of compliant mechanisms actually belongs to a stress-strain field problem.By using finite element analysis.the compliance coefficients of the stage supported by ring are calculated.Finally,a case is inverstigated to exemplity these formulae.     

Enhancing cellular immune response to HBV M DNA vaccine in mice by codelivery of interleukin-18 recombinant

Objective: To investigate the effect of interleukin-18 (IL-18) on immune response induced by plasmid encoding hepatitis B virus middle protein antigen and to explore new strategies for prophylactic and therapeutic HBV DNA vaccines. Methods: BALB/c mice were immunized with pCMV-M alone or co-immunized with pcDNA3-18 and pCMV-M and then their sera were collected for analysing anti-HBsAg antibody by ELISA; splenocytes were isolated for detecting specific CTL response and cytokine assay in vitro. Results: The anti-HBs antibody level of mice co-immunized with pcDNA3-18 and pCMV-M was slightly higher than that of mice immunized with pCMV-M alone, but there was not significantly different (P>0.05). Compared with mice injected with pCMV-M, the specific CTL cytotoxity activity of mice immunized with pcDNA3-18 and pCMV-M was significantly enhanced (P<0.05) and the level of IFN-γ in supernatant of splenocytes cultured with HBsAg in vitro was significantly elevated (P<0.05) while the level of IL-4 had no significant difference (P>0.05). Conclusion: The plasmid encoding IL-18 together with HBV M gene DNA vaccines may enhance specific TH1 cells and CTL cellular immune response induced in mice, so that IL-18 is a promising immune adjuvant.     

甲基橙褪色光度法测定加碘盐中微量碘

基于硫酸介质中,在溴化钾催化作用下,碘酸根氧化甲基橙褪色作用,据此建立了测定碘的动力学光度法.结果表明,最大吸收波长为507 nm时,碘酸钾浓度在0~1.6 mg/L内呈线性关系,表观摩尔吸收系数为9.17×104L.mol-1cm-1.方法用于加碘盐中碘(V)的测定,结果令人满意.     

Dependence of nucleotide physical properties on their placement in codons and determinative degree

INTRODUCTION It is well-known from the genetic code structure that there exists a connection between codons and physical and chemical amino acid properties (Lewin, 1983). Codon properties in turn are defined by physical properties of the nucleotides from which they are compounded (Ratner, 1985; 2000). The most important properties of nucleotides as complex three-dimensional molecules are their dipole moment, heat of formation and energy of the most stable con- formation (Schneider and Be…     

DNA甲基化异常与肿瘤发生的研究

DNA甲基化是真核生物DNA的正常内源性修饰方式 ,它由DNA甲基化转移酶催化发生。但DNA甲基化异常在肿瘤发生中却起着极为重要的作用 ,它主要表现为抑癌基因高甲基化所致的基因失活和原癌基因低甲基化所致的基因激活 ,引起与细胞增殖、分化相关基因表达异常 ,造成细胞恶变形成肿瘤。文章总结了DNA甲基化异常对肿瘤相关基因表达的影响 ,在肿瘤发生中的可能机制及针对高甲基化的治疗方案     

微型同位素辐射能收集器二维悬臂梁机电建模

基于欧拉-伯努利梁理论,设计了一种新型的微型同位素辐射能收集器二维机电模型。该模型解决了一维机电模型无法涵盖的诸多问题。算例分析表明:本模型能较准确地描述实验过程和预测实验结果。通过对结果进行分析,得出了影响微悬臂梁的位移、梁的往返周期和梁中存储能量的一系列因素。     

“观察DNA和RNA在细胞中的分布”实验的改进研究

本文对＂观察DNA和RNA在细胞中的分布＂实验提出了改进方法：人的口腔上皮细胞可直接染色观察,若要烘干必须用低温,不需用HC l进行水解;洋葱鳞片叶内表皮细胞应先用质量分数为4%的HC l溶液水解,然后用质量分数为1%的NaHCO3溶液清洗。通过改进,染色现象明显。     

微电网仿真与实验系统 Ⅰ.总体设计

从设计、数学建模、仿真等方面研究了微电网仿真与实验系统,在微电网模型基础上,采用美国西部联合电网(Western Systems Coordinating Council,WSCC)3机9节点系统作为标准输电网模型,结合硬件仿真实验平台,开发出微电网仿真与实验系统。该系统包括风力发电系统、光伏发电系统、并网接入系统、模拟输配电系统、负荷和仿真实验平台。研制的仿真及实验系统能够稳定可靠运行,实验证实了模型的正确性。应用表明,系统设计合理、功能完善,各方面性能能够满足微电网仿真和实验的要求。