Mass spectrometry-based protein-protein interaction techniques and their applications in studies of DNA damage repair

Proteins are major functional units that are tightly connected to form complex and dynamic networks.These networks enable cells and organisms to operate properly and respond efficiently to environmental cues.Over the past decades,many biochemical methods have been developed to search for protein-binding partners in order to understand how protein networks are constructed and connected.At the same time,rapid development in proteomics and mass spectrometry(MS)techniques makes it possible to identify interacting proteins and build comprehensive protein-protein interaction networks.The resulting interactomes and networks have proven informative in the investigation of biological functions,such as in the field of DNA damage repair.In recent years,a number of proteins involved in DNA damage response and DNA repair pathways have been uncovered with MS-based protein-protein interaction studies.As the technologies for enriching associated proteins and MS become more sophisticated,the studies of protein-protein interactions are entering a new era.In this review,we summarize the strategies and recent developments for exploring protein-protein interaction.In addition,we discuss the application of these tools in the investigation of protein-protein interaction networks involved in DNA damage response and DNA repair.     

DNA’s new avatar as nanoscale construction material

DNA is fast taking on a new aspect utilizing it’s physical property of persistence length and chemical property of base pairing to build architectures in 1D, 2D and 3D. This field is called structural DNA nano-technology and is poised to revolutionize several areas ranging from materials science to cell biology.     

硅微电子技术物理极限对策探讨

根据国际半导体技术蓝图(ITRS)发布的未来半导体工艺技术预测,2016年世界集成电路主流工艺线宽为22纳米,2022年达到10纳米。届时,以硅为基础的微电子技术发展所遵循的摩尔定律将不再适用;为此,必须发展基于全新原理的新技术,以满足人类不断增长的对信息量的需求。本文首先介绍硅材料国内、外的发展现状与趋势,进而讨论后摩尔时代的微电子技术发展可能采取的对策,最后展望基于全新原理的纳米电子、分子电子、光计算和量子信息技术的发展前景。     

灿烂甲酚蓝标记分光光度法测定核酸

研究了灿烂甲酚蓝与小牛胸腺DNA相互作用，提出了测定ctDNA的新的光度分析法。室温、pH8．1tris-HCl的条件下，ctDNA的加入使灿烂甲酚蓝在其最大吸收波长636nm处的吸光度明显下降，下降的程度与ctDNA的含量呈线性关系，ctDNA质量浓度在0～3．0μg／ml范围内与△A呈线性关系，线性回归方程为△A=0．0032 0．0984C(μg/ml）(r=0．9987)，方法的检出限（3σ／k)为0．125μg/ml。该方法具有仪器简单，快速，选择性好等特点，对合成样品中ctDNA测定，RSD％=1．5～1．9，回收率在101％～104％，结果令人满意。     

计算机基础类课程网络空间构建

《计算机文化基础》为当代大学生的一门必修课程,其操作实践性强,传统教学模式已不能满足教师与学生的需求。本文笔者从国内外研究现状分析,结合计算机基础类课程特点,探讨了网络学习空间的构建,并给出了具体方案与实施成果。     

线阵CCD在检测运动物体中的应用

根据CCD交汇测量原理和实际设计需要,综合分析了交汇测量中一些关键技术与问题,并对采集测 量系统进行了理论分析和实验研究,对于测量运动物体瞬时位置有重要意义。     

微课在《自动变速器电控原理与维修》课程中的设计与实践

本课题选择《自动变速器电控原理与维修》课程中的重要知识点和技能点作为微课教学的设计对象,分析了课程的现状及特点,在此基础上提出了针对本课程的制作流程、设计方案与实践方法,为自动变速器电控原理与维修课程教学改革提供了新思路。     

基于遗传算法的阵列相位误差校正

针对声纳水听器阵列比较突出的相位误差,研究了一种基于遗传算法的阵列校正方法。该方法利用无相位误差时阵列协方差矩阵具有Toeplitz结构的特性构造代价函数,并以此作为遗传算法中进化个体的适应度函数,由算法搜索的最优个体得到阵元相位误差的估值。计算机仿真结果表明,该方法可以明显地减小阵列相位误差对MUSIC算法DOA估计结果的影响。     

Effects of medium composition on the production of plasmid DNA vector potentially for human gene therapy

INTRODUCTION The difficulties associated with large-scaleproduction of biotherapeutics provide a constantchallenge to the biotechnology industry. FDA hadadded “therapeutic DNA plasmid vectors” to the listof well-characterized biotechnology product (DoHHs,1996), and gene therapy has moved rapidly fromlaboratory scale to clinical trials. It is urgent to de-velop new protocols to obtain high-quality plasmidswith high yields and minimal or no contamination ofRNA and chromosomal D…