子宫颈癌疫苗研究新进展

子宫颈癌是妇女中最常见的恶性肿瘤之一。人乳头状瘤病毒感染是子宫颈癌发生的基本因素,预防人乳头状瘤病毒感染就可以避免子宫颈癌的发生,理想的预防方法是应用人乳头状瘤病毒疫苗。目前预防和治疗宫颈癌的疫苗类型有载体类、抗原相关类、细胞类等。本文综述了上述疫苗的作用机理及研究进展。并对未来宫颈癌的治疗进行了展望。     

Mass spectrometry-based protein-protein interaction techniques and their applications in studies of DNA damage repair

Proteins are major functional units that are tightly connected to form complex and dynamic networks.These networks enable cells and organisms to operate properly and respond efficiently to environmental cues.Over the past decades,many biochemical methods have been developed to search for protein-binding partners in order to understand how protein networks are constructed and connected.At the same time,rapid development in proteomics and mass spectrometry(MS)techniques makes it possible to identify interacting proteins and build comprehensive protein-protein interaction networks.The resulting interactomes and networks have proven informative in the investigation of biological functions,such as in the field of DNA damage repair.In recent years,a number of proteins involved in DNA damage response and DNA repair pathways have been uncovered with MS-based protein-protein interaction studies.As the technologies for enriching associated proteins and MS become more sophisticated,the studies of protein-protein interactions are entering a new era.In this review,we summarize the strategies and recent developments for exploring protein-protein interaction.In addition,we discuss the application of these tools in the investigation of protein-protein interaction networks involved in DNA damage response and DNA repair.     

两种计划免疫苗种需求预测模型

全文分两部分,第一部分指出计划免疫苗种需求与总和生育率及生育模式的直接关系,应用一种婴儿出生数计算式及胎次率人口系统模型,提出一种苗种需求模型;第二部分利用数方法,提出一种比较实用的苗种需求模型。     

DNA’s new avatar as nanoscale construction material

DNA is fast taking on a new aspect utilizing it’s physical property of persistence length and chemical property of base pairing to build architectures in 1D, 2D and 3D. This field is called structural DNA nano-technology and is poised to revolutionize several areas ranging from materials science to cell biology.     

DNA序列的3D图形表示（英文）

本文介绍一种新的DNA序列的三维图形表示，它可以避免相应空间图形的交叉和重叠。该图形表示的好处还在于，它仅仅有两种需要计算的可能情况，而在其它的三维表示中，往往有三种需要计算的情况。究其应用，我们对两种可能产生的表示情况，通过计算十二种不同物种间编码序列对应的L/L矩阵产生的正规特征值，构造一个2-维向量来检测上述物种间的相似性与非相似性。     

灿烂甲酚蓝标记分光光度法测定核酸

研究了灿烂甲酚蓝与小牛胸腺DNA相互作用，提出了测定ctDNA的新的光度分析法。室温、pH8．1tris-HCl的条件下，ctDNA的加入使灿烂甲酚蓝在其最大吸收波长636nm处的吸光度明显下降，下降的程度与ctDNA的含量呈线性关系，ctDNA质量浓度在0～3．0μg／ml范围内与△A呈线性关系，线性回归方程为△A=0．0032 0．0984C(μg/ml）(r=0．9987)，方法的检出限（3σ／k)为0．125μg/ml。该方法具有仪器简单，快速，选择性好等特点，对合成样品中ctDNA测定，RSD％=1．5～1．9，回收率在101％～104％，结果令人满意。     

兔病毒性出血症和巴氏杆菌病灭活二联苗的研究与应用

用感染RVHD病兔的肝、脾、肾以及从鸡、兔混养暴发巴氏杆菌病兔体分离的细菌研制的甲醛灭活二联苗，免疫健康易感兔，于免疫后对RVHD强毒攻击第3天保护车达50％（6／12），第5天达100％（15／15），持续保护6个月以上，抗体动态测定与攻毒结果一致；对巴氏杆菌攻击第7、15天的保护率分别达75％（9／12）、91．7％（11／12）．6个月保护车仍达72．2％（13／18）。     

Effects of medium composition on the production of plasmid DNA vector potentially for human gene therapy

INTRODUCTION The difficulties associated with large-scaleproduction of biotherapeutics provide a constantchallenge to the biotechnology industry. FDA hadadded “therapeutic DNA plasmid vectors” to the listof well-characterized biotechnology product (DoHHs,1996), and gene therapy has moved rapidly fromlaboratory scale to clinical trials. It is urgent to de-velop new protocols to obtain high-quality plasmidswith high yields and minimal or no contamination ofRNA and chromosomal D…     

<生物的遗传>一节的教学后记

遗传学基础知识是高中生物课的重要内容,教好它对培养学生献身生命科学精神有重要意义.