对国家田径队运动器材及装备现状的调查研究

通过对我国国家田径队器材配备的调查。发现：教练员和运动员对进口器材的质量满意程度很高;国家田径队的训练器材配备基本满足训练的需要,但其配置标准有待提高。科研、医疗器材的配备存在严重的不足,一定程度上影响了田径运动水平的提高;国内田径器材生产厂商的器材质量应加大科技含量,以达到国际先进水平。并针对相关问题提出了几点建议。     

消化系恶性肿瘤浸润淋巴细胞免疫组化研究

应用免疫组织化学PAP法及多种单克隆抗体,对12例常见消化系统恶性肿瘤进行了原位研究。结果显示:①TIL在肿瘤局部主要分布于癌间质区,而癌实质区仅有极少数TIL浸润;②TIL中的主导细胞为CD_3~ T细胞,且无论在实质区还是在间质区CD_4~ 细胞均少于CD_8~ ,癌间质区CD_4~ /CD_8~ 比值大于癌实质区;③无论在癌实质区还是在癌间质区,均有部分CD_(25)~ 或HLA-DR~ TIL存在,对于这些变化的内在机制文中也作了探讨。     

钙通道阻断剂对家兔起搏细胞自发活动的作用

目的 :为进一步阐明Ca2 + 通道在心脏起搏中所起的作用 ,及左心室流出道 (主动脉前庭 )自律细胞的特性。方法 :利用常规的玻璃微电极细胞内记录技术 ,观察了 2 6例家兔窦房结和左心室流出道自律细胞动作电位的特征。以分析钙通道阻断剂 -维拉帕米 (VER)和氯化镍 (NiCl2 )对窦房结和流出道自律细胞的影响。结果 :(1)用 1μmol/LVER灌流后窦房结及主动脉前庭自律细胞的APA、Vmax、MDP绝对值、VDD、RPF均明显下降 ,APD90 延长 (P <0 .0 5 )。 (2 )用 180 μmol/LNiCl2 灌流后两自律细胞自发慢反应电位的VDD、APA、Vmax、RPF均明显下降 (P <0 .0 1) ,而且窦房结细胞APD90 也显著延长 ;二者的MDP、APD50 均无明显改变 (P >0 .0 5 )。结论 :钙通道阻断剂对窦房结起搏细胞和主动脉前庭自律细胞动作电位 0相和 4相去极离子流有明显抑制作用。     

谷氨酰胺对运动性免疫抑制的作用

长时间运动应激能引起血浆谷氨酰胺的浓度下降并发生短暂免疫抑制,从而增加运动员上呼吸道感染的发病率和胃肠疾病的发生率.运动员补充谷氨酰胺后,疾病的发生率有所下降,这与谷氨酰胺在免疫系统中的作用密切相关.对运动性免疫抑制的发生和耐力运动后疾病的发生情况作一综述,并探讨了补充谷氨酰胺的作用及作用的机制.     

司帕沙星治疗老年人下呼吸道感染临床观察

目的：探讨司帕沙星对老年人下呼吸道感染的治疗作用。方法：选择2004．1～2005．6月于我院治疗的74例老年下呼吸道感染的患者用司帕沙星治疗．观察治疗效果。结果：司帕沙星治疗老年人下呼吸道感染总有效率为93．24％，细菌清除率为92．68％，不良反应发生率为7．69％。结论：司帕沙星是一个治疗下呼吸道感染安全、有效的药物。     

经尿道前列腺电切术治疗前列腺增生评价

目的评价经尿道前列腺电切术（TURP）治疗前列腺增生症（BPH）的疗效。方法采用德国STORZ电切镜施行经尿道前列腺电切术治疗BPH 2 530例。结果 2 286例获得1年以上随访,最大尿流率由术前6.9±1.2 mL/s上升到术后14.0±2.2 mL/s,残余尿量由术前145.6±33.2 mL下降至28.6±14.2 mL,手术前后有显著差异。术后并发症：暂时性尿失禁196例;肾盂肾炎31例;附睾炎21例;继发性出血32例;会阴水肿5例;残留前列腺组织28例;尿路感染23例;切穿前列腺包膜11例,其中6例出现TUR综合征,死亡2例。结论 TURP是治疗BPH最有效的方法之一,创伤小、出血少、恢复快,但手术操作的技术水平是治疗的难点和关键。     

Identification of genetic variants the CCKAR gene and based on body measurement and carcass quality characteristics in Qinchuan beef cattle (Bos taurus)

BackgroundThis study aimed to explore genetic polymorphisms of the CCKAR gene and their relationship with the growth and development of Qinchuan cattle which could be used as molecular markers for the improvement of the breeding of Qinchuan cattle.ResultsHere, we have identified seven single nucleotide polymorphisms (SNPs) at loci g. 1463 C>G; g. 1532 T>A; g. 1570 G>A; g. 1594 C>A; g. 1640 T>C; g. 1677 G>C; and g. 1735 C>T in the coding region of the bovine CCKAR gene. The frequencies identified on allelic and genotypic characteristics have shown that all seven SNPs diverged from the Hardy-Weinberg-Equilibrium. The SNP2, SNP3, SNP6 and SNP7 had the lowest polymorphism information content values, and remaining SNPs were found to be moderate (0.25 < PIC < 0.50). The genotype CG in SNP1 at loci g.1463 C>G had the greatest association with WH, HW, CD and CCF, while the genotype TA at the very same loci was associated with BFT, ULA and IMF content in Qinchuan cattle. The CCKAR gene expression level in adipose tissue, small intestine, liver and skeleton muscle was found to be higher, whereas, the expression level of mRNA in organs of other digestive system including reticulum, abomasum and omasum was moderate. Some expression of CCKAR mRNA was found in the large intestine, kidney and rumen.ConclusionsIn summary, our finding suggested that the CCKAR gene could be used as a potential candidate for the improvement of carcass quality and body measurements of Qinchuan cattle.How to citeNurgulsim K, Raza SHA, Khan R, et al. Identification of genetic variants the CCKAR gene and based on body measurement and carcass quality characteristics in Qinchuan beef cattle (Bos taurus). Electron J Biotechnol 2021;51. https://doi.org/10.1016/j.ejbt.2021.02.001     

鲍曼不动杆菌医院内下呼吸道感染危险因素配比病例对照研究

采用１：１配比病例对照研究方法,将９４例临床确诊为鲍曼不动杆菌医院下呼吸道感染的病例,同时配以感染对照和非感染对照进行分析,探讨鲍曼不动杆菌医院下呼吸道感染危险因素,多因素Ｌｏｇｉｓｔｉｃ回归分析表明：病例和非感染病例比较显示,病情危重、气管插管、机械通气、雾化吸入、３种以上抗生素疗程超过２周、ＣＯＰＤ、重症监护和化疗为鲍曼不 直菌医院下呼吸道感染的危险因素;病例与病例对照比较,则气管切开、机械通     

Lactoferrin in cervical mucus as a biochemical marker for inflammation

Lactoferrin, an iron-binding protein, has been proposed to act as an anti-infective agent and has been used as a diagnostic marker in several inflammatory disorders. A sensitive enzyme-linked immunosorbent assay developed earlier was used for lactoferrin estimation in cervical mucus. A brief study was undertaken to observe if lactoferrin is detectable in cervical mucus and to correlate its level with reproductive tract infection, if present. One hundred and twelve cervical mucus samples were collected from healthy as well as infected females. Some of these females were using CuT-200 intrauterine copper devices for contraception. Women were at different phases of their menstrual cycle. The presence of detectable amount of lactoferrin in cervical mucus was confirmed. The average level of lactoferrin in cases either with clinical symptoms of cervicitis or with proven infection by PAP smear was significantly (t=7.6, P<0.01) higher than the normal controls. CuT users have higher (P<0.01) mean level of lactoferrin than corresponding non-users.     

瓦氏黄颡鱼消化系统组织学的初步研究

采用光镜技术对瓦氏黄颡鱼的消化道和消化腺进行了初步研究。结果表明：消化道分化为明显的食道、胃和肠。食道黏膜为复层上皮，其中含有杯状细胞和味蕾；胃U形，粘膜表层为单层柱状上皮，贲门部和盲囊部上皮下有一厚层胃腺组织，肠粘膜也为单层柱状上皮，其中散布较多的杯状细胞。消化腺包括肝脏和胰腺，肝小叶分界不明显，肝细胞索明显，胰腺外分泌部由许多腺泡组成，内分泌部即胰岛分散存在于外分泌部之间。