碱性苦味酸法和酶法测血清肌酐的方法学比较

[目的]了解酶法和碱性苦味酸法测定血清肌酐结果的偏倚大小,对两种方法进行比较.[方法]用生化仪器OLYMPUSAU1000分别进行酶法和碱性苦味酸法测定血清肌酐.对两种方法进行重复性实验、相关性实验、线性比较和回收试验.[结果]酶法和碱性苦味酸法的重复性实验数据(x±s)266±2.77μmol／L和273.5±3.30μmol／L,其变异系数分别为1.04％、1.21％;两者的回归方程式yJaffe=1.01X二步酶法-1.28;酶法的线形范围远大于碱性苦味酸法;在回收试验中两者的回收率分别为98.5％、94.5%.[结论]酶法和苦味酸法有较好的相关性,酶法比碱性苦味酸法线性范围宽,特异性和灵敏度高,测定重复性好,是比较理想的方法.临床上酶法将逐渐代替苦味酸法测定血清肌酐.     

浅析一元多项式相加问题在计算机中的实现

以单链表这种数据结构为工具，详细描述了一元多项式在计算机中的表示以及两个一元多项式相加运算在计算机中的实现。     

基于CKAN的政府数据开放平台构建

[目的/意义]本研究针对我国地方政府数据开放平台面临的挑战，设计并构建基于CKAN的政府数据开放平台，为我国政府数据开放平台的建设和改进提供参考。[方法/过程]在对CKAN的应用及特征进行概述的基础上，分析CKAN具有的核心功能，探讨CKAN的元数据管理以及关联数据集的API发布，最后给出平台安装的步骤和策略。[结果/结论]充分发挥CKAN平台的核心功能、元数据管理以及关联数据集的API发布等方面的优势，结合Drupal等开源内容管理框架来构建政府数据开放平台才是最优选择。     

我国香蕉资源的加工利用研究

香蕉是有名的热带、亚热带水果，我国南方香蕉资源十分丰富，近几年来发展很快，产量的剧增给香蕉的加工利用和产品开发提出了新的课题。本文比较详细地介绍了喷雾干燥法生产香蕉粉、酶解法制香蕉汁和香蕉片的生产工艺，简述了香蕉皮综合利用的途径，报道了香蕉粉营养成分的化     

试论汉语谓词性短语的语法结构界定问题

针对汉语动宾短语和连动短语的辨识问题立论,结合汉语教学中难以界定的实例,将其相关的纠葛问题逐一阐释,剖析了若干彼此难分的情形,特别是对于那些处于中介状态的语法现象,给予了理性的界定。     

Irreplaceable ISSN: From 2007 to the Future

International Organization for Standardization (ISO) standard 3297 governs the assignment of International Standard Serial Numbers (ISSNs) for continuing resources worldwide. It is subject to systematic review every five years and, in 2017, an ISO Working Group was set up to review the standard. This working group took the decision to survey the views of stakeholders including publishers, libraries, and others. This article reports on the global survey that was undertaken, describing the questions and answers about which the committee sought opinions. It also sought details on the respondents to provide context. The replies are summarised in chart form with conclusions and an appraisal of the key issues, which suggest that whilst respondents are supportive of expanding the ways in which the ISSN is deployed, they are less convinced of the need for more ISSNs for any given title. They are split on the need for the structure of the ISSN to be revised but there is strong support for the notion of a “Family ISSN” that would describe resources that are linked through, for example, title changes or geography or language. The article notes the next steps to be taken.     

Kinetic analysis of γ-glutamyltransferase reaction process for measuring activity via an integration strategy at low concentrations of γ-glutamyl p-nitroaniline

At 0.12 mmol/L γ-glutamyl p-nitroaniline (GGPNA), an improved integrated method was developed for kinetic analysis of γ-glutamyltransferase (GGT) reaction process and the integration with the classical initial rate method to measure serum GGT. For the improved integrated method, an integrated rate equation, which used the predictor variable of reaction time and considered inhibitions by both GGPNA and products, was nonlinearly fit to GGT reaction processes. For the integration strategy, classical initial rates were estimated when GGPNA consumption percentages were below 50%; otherwise, maximal reaction rates of GGT were estimated by the improved integrated method and converted into initial rates according to the differential rate equation at 0.11 mmol/L GGPNA. The integration strategy was validated using optimized GGT kinetic parameters and 10-s intervals to record reaction curves within 8.0 min. By the integration strategy, there was a linear response from 0.9 to 32.0 U/L GGT, coefficients of variation were below 3.5% for GGT from 8.0 to 32.0 U/L (n=5), and GGT activities in clinical sera responded linearly to their classical initial rates at 2.00 mmol/L GGPNA with an expected slope. Therefore, the integration strategy was successful in measuring GGT at 0.12 mmol/L GGPNA.     

Stomatocyte structural color-barcode micromotors for multiplex assays

Artificial micromotors have a demonstrated value in the biomedical area. Attempts to develop this technology tend to impart micromotors with novel functions to improve the values. Herein, we present novel structural color-barcode micromotors for the multiplex assays. We found that, by rapidly extracting solvent and assembling monodispersed nanoparticles in droplets, it could form stomatocyte colloidal crystal clusters, which not only showed striking structural colors and characteristic reflection peaks due to their ordered nanoparticles arrangement, but also provided effective cavities for the integration of functional elements. Thus, the micromotors with catalysts or magnetic elements in their cavities, as well as with the corresponding structural color coding, could be achieved by using the platinum and ferric oxide dispersed pre-gel to fill and duplicate the stomatocyte colloidal crystal clusters. We have demonstrated that the self-movement of these structural color-barcode micromotors could efficiently accelerate the mixing speed of the detection sample and greatly increase the probe–target interactions towards faster and more sensitive single or multiplex detection, and the magnetism of these barcode micromotors enables the flexible collection of the micromotors, which could facilitate the detection processes. These features make the stomatocyte structural color-barcode micromotors ideal for biomedical applications.     

2009年以来本体映射系统模型研究综述

本体映射系统分为通用映射系统和领域映射系统两大类型,从模型和流程视角综述2009年RiMOM、ASMOV、DSSim、OntoMap、OntSE五种本体映射系统模型,认为本体映射研究在关联数据等新兴前沿领域的应用有待进一步深化。     

情报学进展系列论文之七 数据密集型科学研究范式的兴起与情报学的应对

以数据密集型计算为特征的科学研究第四范式正在兴起,文章分析了第四范式的提出及其兴起的社会背景,研究了该范式的内涵和价值,总结了目前图书情报领域针对数据密集型科学研究范式的理论和实践,探讨了作为科学学科的情报学的应对措施.