天门冬氨酸钾镁消除运动疲劳的作用机制

天门冬氨酸钾镁(PMA)的主要作用是加速三羧酸循环中的有氧代谢过程;消除运动中产生的氨．降低血氨浓度;消除氧自由基;提高镁的含量和Na~ ／K~ -ATP酶的活性,维持细胞膜内外离子浓度等,补充天门冬氨钾镁对抵抗疲劳产生,提高运动能力有很好的效果。本文较系统地论述了天门冬氨酸钾镁抗疲劳的生物学机制。     

张家口市SARS患者、疑似病例、留观及密切接触医护人员血清中IgG、IgM抗体测定

目的 :为临床诊断及排除严重急性呼吸综合征 (SARS)提供依据和对留观及密切接触医护人员有无感染作初步调查。方法 :用酶联免疫吸附测定 (ELISA) 5 3例临床诊断病例、2 9例疑似病例、6 3例留观及 114例密切接触医护人员的血清中SARS冠状病毒IgG、IgM抗体。结果 :SARS临床诊断病例IgG抗体阳性 2 1例 ,IgM抗体阳性 18例 ,其中有 17例IgG、IgM抗体均为阳性 ,总阳性率为 4 1.5 % ;留观人员IgG抗体阳性 1例 ;其他均为阴性。 结论 :ELISA测定血清抗SARS冠状病毒抗体阳性病例可确诊感染病毒 ,对于观察期后 (一般为 2 1d)人员 ,阴性结果可以排除感染。     

亚硫酸盐还原酶发酵工艺条件初探

本文对硫酸盐还原菌产亚硫酸盐还原酶的发酵工艺条件进行了初步研究,主要内容包括硫酸盐还原菌发酵条件：碳源、氮源、温度、起始pH、装液量及发酵周期等.实验研究表明,以尿素作为氮源,以可溶性淀粉为碳源,C：N比为1：3,在初始pH7.0的条件下,接种量为5%,装液量为1：3（v/v）,35℃摇床培养42h,酶活力可达到16.2U/mL.     

在单链表上实现一元多项式的表示和相加

以单使表这种数据结构为工具，详细描述了一元多项式在计算机中的表示以及两个一元多项式相加运算在计算机中的实现。     

基于存储结构的Dijkstra算法优化

在求解最短路径时经常使用经典的Dijkstra算法,但在实际应用中在计算最短路径长度时需要进行大量的数据比较,而当图中两顶点之间的距离是∞时,是没有必要进行比较的。本文从存储结构上讨论如何对Dijkstra算法进行优化,尽量减少数据比较次数。     

Characterization of cellulose acetate micropore membrane immobilized acylase I

This paper describes an innovative method for the immobilization of acylase I, which was entrapped into the CA-CTA micropore membrane. The most suitable casting solutions proportion for immobilizing the enzyme was ob tained through orthogonal experiment. Properties of the enzyme membrane were investigated and compared with those of free enzyme and blank membrane. The thermal stability and pH stability of the enzyme inside the membrane were changed by immobilization. The optimum pH was found to be 6.0, which changes 1.0 unit compared with that of free acylase I. The optimum temperature was found to be about 90 ℃C, which is higher than that of free acylase I (60 ℃). Experimental results showed that immobilization had effects on the kinetic parameters of acylase I.     

视网膜色素上皮DNA蓝光损伤及维生素E的保护作用

目的：探讨维生素E对视网膜色素上皮DNA蓝光损伤的保护作用。方法：视网膜色素上皮细胞分为3组：正常组（不照光），实验组（蓝光照射40min），维生素E组（蓝光照射同时给予维生素E）。采用火箭电泳的方法对各组视网膜色素上皮DNA损伤进行分析。结果：各组火箭电泳尾部DNA含量分别为18．44％，54．42％和22．16％。各组差异有显著性意义（P＜0．01）。结论：蓝光能对视网膜色素上皮的DNA产生损伤，维生素E对损伤能起到一定的保护作用。     

基于共词分析和社会网络分析的国内外关联数据研究探析

准确把握国内外关联数据的研究方向及研究热点, 梳理分析国内外关联数据的核心作者及核心团体, 有效了解国内关联数据研究存在的问题与差距, 以期为国内关联数据的后续发展提供借鉴与参考。以国内的CNKI和国外的Web of Science两个数据库中2006-2015年的相关文献为数据来源, 运用共词分析和社会网络分析方法, 分析整理该领域的研究方向及作者合作关系, 并将国内外的相关情况进行对比分析。发现国内的研究相较国外存在一定的差距, 认为在后续的发展过程中, 国内应在深化已有研究方向的基础上, 积极开拓新的研究方向, 并加强作者和机构之间的合作关系, 促进整个领域的知识传播。     

Comparison of two methods of estimation of low density lipoprotein cholesterol, the direct versus friedewald estimation

Current recommendations of the Adult Treatment Panel and Adolescents Treatment Panel of National Cholesterol Education Program make the low-density lipoprotein cholesterol (LDL-C) levels in serum the basis of classification and management of hypercholesterolemia. A number of direct homogenous assays based on surfactant/solubility principles have evolved in the recent past. This has made LDL-C estimation less cumbersome than the earlier used methods. Here we compared one of the direct homogenous assays with the widely used Friedewald’s method of estimation of LDL-C to see the differences and correlation. We used direct homogenous assay kit to estimate serum LDL-C and high-density lipoprotein cholesterol (HDL-C). Serum Triglyceride (TG) and Total Cholesterol (TC) was estimated and using Friedewald’s formula LDL-C was calculated. The LDL-C level obtained by both methods in 893 fasting serum samples were compared. The statistical methods used were paired t-test and Pearson’s correlation. There was significant difference in the mean LDL-C levels obtained by the two methods at the TG levels <200 mg/dl (p<0.02) and TC levels >150 mg% (p<0.001). The correlation coefficient (r) between Friedewald’s and direct assay estimation was 0.88. Friedewald’s method classified 23.5 % of patients as high cardiac risk whereas there were 17.58% by direct assay. Both had good correlation even though the serum triglyceride and total cholesterol levels affect the difference in LDL-C estimated by both methods. Taking into account the cost and performance, Friedewald’s method is as good or even better for classifying and managing patients.