Early lethality of shRNA-transgenic pigs due to saturation of microRNA pathways

RNA interference (RNAi) is considered as a potential modality for clinical treatment and anti-virus animal breeding. Here, we investigate the feasibility of inhibiting classical swine fever virus (CSFV) replication by short hairpin RNA (shRNA) in vitro and in vivo. We generate four different shRNA-positive clonal cells and two types of shRNA-transgenic pigs. CSFV could be effectively inhibited in shRNA-positive clonal cells and tail tip fibroblasts of shRNA-transgenic pigs. Unexpectedly, an early lethality due to shRNA is observed in these shRNA-transgenic pigs. With further research on shRNA-positive clonal cells and transgenic pigs, we report a great induction of interferon (IFN)-responsive genes in shRNA-positive clonal cells, altered levels of endogenous microRNAs (miRNA), and their processing enzymes in shRNA-positive cells. What is more, abnormal expressions of miRNAs and their processing enzymes are also observed in the livers of shRNA-transgenic pigs, indicating saturation of miRNA/shRNA pathways induced by shRNA. In addition, we investigate the effects of shRNAs on the development of somatic cell nuclear transfer (SCNT) embryos. These results show that shRNA causes adverse effects in vitro and in vivo and shRNA-induced disruption of the endogenous miRNA pathway may lead to the early lethality of shRNA-transgenic pigs. We firstly report abnormalities of the miRNA pathway in shRNA-transgenic animals, which may explain the early lethality of shRNA-transgenic pigs and has important implications for shRNA-transgenic animal preparation.     

全球1918年大流感病毒研究发展态势文献计量分析

检索 Web of Science 数据库中1995-2014年发表的1918年大流感病毒研究文献,利用 VOSviewer 软件绘制该研究领域的机构合作网络、作者合著网络、关键词热度图谱、关键词聚类图谱、参考文献共被引图谱,对该研究领域的研究热点和发展态势进行分析,为未来流感大流行防控提供重要的信息。     

基于引导型病毒的汇编语言教学案例设计

汇编语言是计算机专业本科生的一门重要必修课,但又是一门学生难学,教师难教的课。现有的大部分教学案例缺乏综合性,与实际工作联系不紧密。针对这些问题,从课程群建设角度出发,设计一个基于引导型病毒的教学案例,让学生直观地感受到汇编语言在信息安全领域的应用。介绍了案例的基本原理、实现,在教学过程中的使用方法和需要注意的地方,为丰富汇编语言教学案例提出了一种可行的思路。     

改进的乙肝病毒感染动力学模型研究

基于Nowak等提出的不带效应细胞的乙肝病毒(HBV)感染模型,本文提出了由两个系统组成的数学模型,并分析了该模型的动力学行为,最后进行了数值模拟,通过数值模拟发现,该模型对慢性HBV感染的拉米夫定治疗效果提供了可能的解释,特别是可以解释为什么Nowak等人的患者的血浆中的病毒数目在停止免疫治疗后又恢复到治疗前的水平.     

浅谈计算机病毒及防范策略

本文简要介绍了当代计算机病毒相关内容,提出了相应计算机病毒防范的措施,以及对计算机病毒未来发展趋势进行了阐述.     

Hepatitis during respiratory syncytial virus infection – a case report

Introduction:

Respiratory syncytial virus (RSV) infection is the most common cause of hospitalization in infants and small children. The aim was to present a 13-months old boy diagnosed with acute airway infection, acute otitis media (AOM) and hepatitis during the RSV-infection.

Material and methods:

Serum catalytic activities of alkaline phosphatase (ALP), aspartate aminotranspherase (AST), alanine aminotranspherase (ALT), gamma glutamyl transpherase (GGT), lactate dehydrogenase (LD), and concentrations of bilirubin were monitored during hospitalization and at control examination.

Results:

The child had clinical signs and symptoms of respiratory failure, AOM, and laboratory findings of virus infection and liver disease. On admission, catalytic activities of enzymes were markedly increased, especially the activity of ALP (10333 U/L, i.e. 24-fold increase in comparison with the upper reference limit). The highest increased in AST (339 U/L, 4.5-fold), ALT (475 U/L, 10.3-fold) and LD (545 U/L, 1.5-fold) were registered on the 3^rd day, and the highest increase in GGT (68 U/L, 3.1-fold) occurred on the 11th day. Seven weeks after discharge AST, ALT, GGT and LD decreased into reference range, and ALP remain mildly increased (478 U/L, 1.1 fold increase). RSV was confirmed in nasal lavage fluid.

Conclusion:

Laboratory results in patient with RSV infection needs to be interpreted in the light of both, respiratory and extrapulmonary manifestations of the infection, respectively.     

从病毒概述中探索病原生物与免疫学教学方法

目的：探索适合中职生的病原生物与免疫学基础教学方法。方法：教学中以病毒概述一章为代表,选取揭阳市卫生学校2012级农村医学专业学生为研究对象,采用图片展示、对比讲解、举例提问、动画演示、数据列举等多方法组合教学。结果：学生主动学习积极、分析能力、测试成绩均显著提高,取得良好成效。结论：与传统的讲授法相比较,多方法组合教学能充分调动学生学习兴趣、增强理解记忆能力、培养临床思维,有效提高教学质量。     

云安全对图书馆网络信息系统安全的影响

在介绍云安全的基础上,结合图书馆网络信息系统特点,探讨了将云安全应用于图书馆网络系统安全的必要条件,并分析了应用云安全后给图书馆工作及服务带来的影响和优势。     

网上审稿的实践与技巧

分析网络审稿中常遇到的一些问题,如怎样制作审稿单、计算机感染病毒、函件乱码、电子稿泄露审稿专家姓名信息等,并提出了解决方法.