Science Letters： A TOM1 homologue is required for multiplication of Tobacco mosaic virus in Nicotiana benthamiana

The AtTOM1 gene ofArabidopsis thaliana had been shown to be essential for the efficient multiplication of Tobacco mosaic virus （TMV） in A. thaliana. In this study, we cloned an AtTOM1-like gene from Nicotiana benthamiana named as NbTOM1. Sequence alignment showed that NbTOM1 is closely related to AtTOM1 homologues of N. tabacum and Lycopersicon esculentum with 97.2% and 92.6% nucleotide sequence identities, respectively. Silencing of NbTOM1 by a modified viral satellite DNA-based vector resulted in complete inhibition of the multiplication of TMV in N. benthamiana. The result suggests that inhibition of NbTOM1 via RNA silencing is a potentially useful method for generating TMV-resistant plants.     

网络病毒感染下振荡攻击数据混淆分离算法

通过对网络病毒感染下振荡攻击数据混淆分离,实现对网络病毒攻击数据的特征定位和提取,有效检测网络病毒数据。传统方法中对网络病毒感染下的振荡攻击数据的检测和分离方法使用行为特征分析和卡尔曼滤波方法,算法受到数据振荡和线性特征干扰的影响,检测性能不好。提出一种基于双线性本征波匹配的振荡攻击数据混淆分离算法,构建网络病毒感染振动攻击模型,提取攻击数据的信号模型特征,根据调解病毒样本序列的线性化程度,确定双线性本征陷波器频率参数和带宽参数,实现对信号的滤波,进而实现混淆分离算法的改进。仿真结果表明,该算法设计的滤波器进行振荡攻击数据的混淆分离,具有较好的抗噪能力和干扰抑制能力,对网络病毒攻击数据的混淆分离均方根误差较小,对病毒感染下的振荡攻击信号的检测性能较高,展示了其优越性能。     

中国小麦花叶病毒运动蛋白基因的克隆及其表达

应用RT-PCR技术从小麦病叶中扩增得到中国小麦花叶病毒(CWMV)的运动蛋白(MP)基因,在大肠杆菌BL21(DE3)plysS中得到大量表达,并以含表达产物的凝胶为抗原,免疫小鼠制备特异性抗血清,同时将MP基因重组到质粒pGBKT7中,电击转化酵母Y187菌株。Westernblot分析表明MP基因在酵母体内能以融合蛋白的形式正确表达,并具有免疫活性,可作为酵母双杂交系统中的“诱饵”质粒。     

实验大鼠引发实验人员感染流行性出血热的诊断分析

对实验ＳＤ大鼠引实验人员感染流行性出血热（ＥＨＦ）进行了诊断分析．从临床表现和血清初步诊断某教研室１名实验人员感染了流行性出血热病毒（ＥＨＦＶ）．在该教研室的实验ＳＤ大鼠中检出ＥＨＦＶ,也从周围捕捉的野鼠中检出ＥＨＦＶ抗体．初步认为,ＥＨＦＶ是从野鼠传染实验大鼠再传染给实验人员而引发ＥＨＦ．     

病毒性肝炎患者血清谷胱甘肽S-转移 酶活性的测定及其临床意义

本文对各类盱脏疾病及50例非肝脏疾病患者的血清谷眺甘肽S-转移酶(GST)及谷丙转氨酶(ALT)进行了检测,结果表明,血清GST活性测定是一项特异性和敏感性均较好的肝功能指标,在甲型肝炎患者中,此酶活性显著升高,且与ALT呈正相关;在乙型肝炎、丙型肝炎、肝硬化患者中GST都不同程度的升高;非肝脏疾病患者中,GST的阳性率低于ALT的阳性率。     

计算机病毒的检测

计算机病毒目前十分猖獗,破坏性很大。本文从不同的同介绍了在ＤＯＳ操作系统下检测计算机病毒的方法。     

黄瓜花叶病毒卫星RNA的研究进展

黄瓜花叶病毒卫星RNA是寄生于黄瓜花叶病毒粒子内的小分子核酸,卫星RNA必须依赖辅助病毒的复制而复制并反过来干扰CMV的复制,从而影响寄主的病状表现;CMV卫星RNA常被用于病毒病生物防治,也是研究小分子RNA与较高级病毒与寄主相互关系较合适的一种模式。近年来黄瓜花叶病毒卫星RNA的研究主要包括CMV卫星RNA对寄主症状的调节作用,卫星RNA的结构、遗传学性质、与辅助病毒互作的分子机制及其在基因工程中的应用。     

计算机反病毒研究

计算机反病毒必须与操作系统紧密结合 ,软硬件相结合才有可能在根本上杜绝计算机病毒的肆虐。     

Complete blood count alterations in COVID-19 patients: A narrative review

Coronavirus disease 2019 (COVID-19) pandemic represents a scientific and social crisis. One of the main unmet needs for coronavirus disease 2019 is its unpredictable clinical course, which can rapidly change in an irreversible outcome. COVID-19 patients can be classified into mild, moderate, and severe. Several haematological parameters, such as platelets, white blood cell total count, lymphocytes, neutrophils, (together with neutrophil-lymphocyte and platelet-lymphocyte ratio), and haemoglobin were described to be associated with COVID-19 infection and severity. The purpose of these review is to describe the current state of the art about complete blood count alterations during COVID-19 infection, and to summarize the crucial role of some haematological parameters during the course of the disease. Decreased platelet, lymphocyte, haemoglobin, eosinophil, and basophil count, increased neutrophil count and neutrophil-lymphocyte and platelet-lymphocyte ratio have been associated with COVID-19 infection and a worse clinical outcome. Our study adds some novelty about the identification of effective biomarkers of progressive disease, and might be helpful for diagnosis, prevention of complications, and effective therapy.     

病毒肺炎合剂HPLC指纹图谱的研究

目的:应用HPLC法研究病毒肺炎合剂的指纹图谱。方法:以绿原酸为参照物,YWG-C18(250mm×4.6mm,10μm)色谱柱,柱温30℃,流动相为0.4%磷酸水溶液-乙腈,线性梯度洗脱,流速1ml/min,检测波长280nm。结果:在该色谱条件下,病毒肺炎合剂水提取液有14个共有特征指纹峰。结论:该方法稳定、可靠、重现性好,可作为质量控制方法。