大麦黄花叶病抗性机理的初步研究

不同抗性的大麦品种 (系 )被黄花叶病毒侵染后 ,叶片中叶绿素、可溶性蛋白质、游离脯氨酸的含量有规律地变化 ,研究结果表明 ,感病后 ,大麦叶片内叶绿素、可溶性蛋白质含量下降 ,感病品种下降幅度大于抗性品种 ;而游离脯氨酸含量增加 ,抗性品种增加的幅度比感病品种大 ,同时发现 ,叶绿素、可溶性蛋白质、游离脯氨酸的含量与病情指数呈显著的负相关。     

Characterization and RNA2 nucleotide sequence of broad bean wilt virus 2 isolate P158

Broad bean wilt virus 2 (BBWV2) isolate P158 was characterized and the complete nucleotide sequence of viral genomic RNA2 was determined. P158 has 30 nm diameter isometric particles. Double immunodiffusion test suggested that P158 has high antigenicity homology with BBWV2 isolate B935. SDS-PAGE result showed that the coat protein of P158 was comprised of two types of polypeptide with molecular weight of 44.7 kD and 21.9 kD, respectively. The genome of P158 was made up of two RNA molecules with the length of 6.0 kb and 3.6 kb, respectively. cDNA of RNA2 was cloned by RT-PCR and sequenced. RNA2 was composed of 3597 nucleotide (nt) residues excluding the poly(A) tail and contained single long open reading frame extending from nt 230 to nt 3424 in the viral sense RNA, and encoded a polyprotein ofMr 119002 (119K). Comparison of the polyprotein and the counterpart of isolate B935 indicated that the polyprotein was cleaved at Q/G (465/466) and Q/A (867/868), to release three mature proteins: a protein of unknown function, large and small subunit. Sequence comparisons of P158 with fabaviruses showed P158 had very high sequence homology with BBWV2 isolates and patchouli mild mosaic virus, but to a less extent with BBWV1 isolates. Project supported by NSFC (No 39770035; 39900005). The GenBank accession number of the sequence reported in this paper is AF228423.     

简论数字图书馆的不足

重点针对数字图书馆在发展之初业已暴露的三大问题,深刻揭示它们可能造成的致命危害,以期引起各位同仁的高度重视,防患于未然.     

家蚕核型多角体病毒p35基因的核苷酸序列分析

对家蚕核型多角体病毒苏州株（ＢｍＮＰＶｓｕ）ｐ３５基因的序列分析表明：ＢｍＮＰＶｓｕ ｐ３５编码序列为８９７ｍｔ,编码２９８ａａ。同源性分析表明：ＢｍＮＰＶｓｕ ｐ３５与ＢｍＮＰＶＴ３、ＡｃＮＰＶ 、ＳＩＮＰＶ在核苷酸水平上同源性分别为９９．５％、９５．１％、８９．３％,在氨基酸水平上的 性分别为９８．７％、８９．４％、７６．４％,显示了杆状病毒ｐ３５基因在进化上的保守性。ＢｍＮＰＶＴ３中位的Ｎ１     

多酸化合物药物化学的研究进展

系统论述了当前国内外对多酸化合物药物特性的研究工作新进展,特别对多金属氧酸盐的抗艾滋病、抗肿瘤、抗病毒、抗风湿等化学理论进行了论述,同时对多金属氧酸盐的药物特点、研究方向和趋势作了分析。     

如何有效预防及解决U盘病毒

U盘病毒是通过感染配置文件autorun.inf来发作,井经过网络和硬件两种途径传播,其种类有柯南头、随机8位数、回收站等病毒,症状表现为每个盘根目录下都有autorun.inf文件,且双击打不开盘.其病毒以预防为主,解决办法是查、杀、清理非法文件和进程,顺藤摸瓜,揪出祸根.     

冰冻圈微生物演变与生物安全

冰冻圈独特而极端的生态环境蕴藏着丰富的微生物资源,保存了关于古微生物多样性和进化历史的珍贵记录。气候变化和人类活动正在对全球生态系统造成显著影响,冰冻圈加速消融缩减使原本冻存其中的微生物被“解封”,一些微生物适应新的环境条件从而复苏存活,随之构成对生态环境和人类健康的未知威胁。为此,亟待联合多学科研究力量,调查研究冰冻圈微生物资源,解析其中病原微生物的致病和传播机制,充分评估休眠微生物复苏释放带来的生物安全风险。     

强杂波环境下网络病毒传播灰阶菌群免疫算法

网络病毒的入侵和传播通常伴随着强杂波信息的掩盖而提高其生存能力,研究一种有效的免疫算法是对抗病毒传播的关键。传统的抵御网络病毒传播的免疫方法采用人工免疫算法,该法基于熵权重属性分类时不能有效抵抗强杂波干扰的影响,抗病毒感染的免疫能力不高。本文提出一种基于自适应实时繁殖策略的灰阶菌群免疫算法,采用分段匹配白化滤波方法滤除杂波信息,构建强杂波环境下病毒传播数学演化模型,以信息熵作为病毒传播免疫分类属性,提出采用灰阶菌群聚类方法进行病毒特征免疫。进行仿真实验表明该算法能有效滤除病毒潜藏的杂波干扰,病毒信息特征菌群得到准确聚类,降低了计算机用户的感染率,实时性和稳定性好。     

Construction and identification of the recombinant of the AAV vector and human interferon-gamma

To construct and identify further a recombinant of Adeno-associated virus and interferon-gamma for gene therapy, the full-length IFN-γcDNA containing signal peptide was amplified by PCR, and then cloned into the pUC18. After screening, the fragment from the positive clone was then subcloned into pwp19. After the correct recombinant was identified by digestion with SacI and BamHI, it was transfected into lymphocyte cell line H9 mediated by calcium phosphate, and the expression of IFN-γ was detected by RT-PCR and ELISA. The result showed that the IFN-γ were expressed in the H9 cells transfected with pwp/IFN-γ. The so constructed recombinant plasmid pwp19/IFN-γ containing the full-length IFN-γ gene was expressed in mammalian cells. Project (39570653) supported by NFSC.     

临床上引起脑炎的几种常见病毒种类

引起人类中枢神经系统疾病（脑炎）的病毒种类有十余科，多个属。症状相似、病原不同。临床诊断困难，不便于学生系统掌握。