新城疫病毒体外诱导S180细胞凋亡的实验研究

目的:探讨新城疫病毒(Newcastle disease virus,NDV)体外对S180肉瘤细胞(简称S180)生长有无抑制和诱导细胞凋亡作用。方法:取培养至对数生长期的S180细胞浓度为5×104/mL和1×106/mL分别接种于96孔板和50mL培养瓶中,加入不同浓度的NDV(1∶20、1∶10、1∶5)作用24h,分别用MTT法检测NDV对S180瘤细胞的生长抑制作用,荧光显微镜和琼脂糖凝胶电泳检测细胞凋亡。结果:经上述不同浓度的NDV作用24h,对S180细胞生长的抑制率依次为10%、26%、39%,各实验组吸光度值(A490)与对照组比较差异均有统计学意义(P<0.05)。荧光显微镜观察实验组培养的S180瘤细胞,出现细胞核固缩及凋亡小体,琼脂糖凝胶电泳见DNA梯形条带。结论:NDV对体外培养S180细胞增殖有明显抑制作用,可诱导S180细胞凋亡。     

CONSTRUCTION AND IDENTIFICATION OF THE RECOMBINANT OF THE AAV VECTOR AND HUMAN INTERFERON-GAMMA

ＩＮＴＲＯＤＵＣＴＩＯＮＡｎＡｄｅｎｏ ａｓｓｏｃｉａｔｅｄｖｉｒｕｓ(ＡＡＶ)ｐａｒｔｉｃｌｅｉｓｃｏｍｐｏｓｅｄｏｆｔｈｒｅｅｓｔｒｕｃｔｕｒａｌｐｒｏｔｅｉｎｓａｎｄａｌｉｎ ｅａｒ ,ｓｉｎｇｌｅ ｓｔｒａｎｄｅｄＤＮＡ (ｓｓＤＮＡ)ｇｅｎｏｍｅｏｆａｐｐｒｏｘｉｍａｔｅｌｙ 4 .7ｋｂ .ＡＡＶｉｓａｄｅｆｅｃｔｉｖｅｈｕ ｍａｎｐａｒｖｏｖｉｒｕｓｔｈａｔｎｏｒｍａｌｌｙｒｅｐｌｉｃａｔｅｓｐｒｏｄｕｃ ｔｉｖｅｌｙｏｎｌｙｉｎｔｈｅｐｒｅｓｅｎｃｅｏｆｈｅｌｐｅｒｆｕｎｃｔｉｏｎｓｐｒｏｖｉｄｅ…     

具免疫应答时滞乙肝病毒的数学模型

据统计,世界有1/3的人口曾经感染乙肝病毒,约有3.5亿人是HBV携带者。如何预防和治疗乙肝一直是社会关注的焦点和医学与数学等交叉学科的重要课题。基于Nowak模型,建立了具免疫时滞因素HBV感染时滞微分方程模型,对该模型的动力学进行了分析,并应用Routh-Hurwitze定理及Lyapunov-Lasalle定理讨论了该模型平衡点的稳定性,分析了免疫时滞对系统动力学性质产生的影响。数值模拟验证了所得到的结果。     

云南禄劝县烤烟成熟期主要病毒病种类及侵染特征初探

采用TAS-ELISA,DAS-ELISA和ID-ELISA 3种检测方法,选用11种病毒病原血清对禄劝烟区烟草成熟期受害典型样本进行检测.结果表明,禄劝烟区烟草成熟期的病毒病具有从单一侵染向复合侵染演变趋势,且病原危害种类以TMV为主,CMV次之,除此之外尚存在TEV,TVBMV等病毒.烟草成熟期受害叶片症状呈现＂复杂化与多样化＂特征,借助先进检测手段、扩大病原血清种类是探索清楚当地烟草成熟期主要病毒病种类的必要措施.     

德宏州人乳头瘤病毒感染与TCT检测相关性分析

目的：探讨人乳头瘤病毒感染（HPV）与液基薄层细胞学（TCT）检测的相关性。方法：对就诊的1608例有宫颈疾患的妇女,采用HPV分型检测技术进行人乳头瘤病毒分型检测和TCT检测,以病理学2004年TBS分级系统为判断标准。结果：1608例做了入乳头瘤病毒检测样本中,阳性645例,阳性率为40．11％;在645例人乳头瘤病毒阳性中有483例同时做了TCT检测,其中ASCUS者118例,高度病变36例,低度病变24例,炎症154例,正常125例,其它病变26例。结论：HPV检测对宫颈病变可进行早期追踪治疗,提高宫颈液基薄层细胞检测的敏感性,早期预防宫颈癌或发现癌前病变。     

NDV感染体外诱导胃癌细胞BGC-823的凋亡

目的 :研究新城疫病毒在体外抗胃癌细胞活性及其与细胞凋亡的关系。方法 :应用倒置显微镜观察细胞形态、MTT法测NDV在体外对BGC - 82 3的抑制和杀伤作用 ,同时用流式细胞术检测胃癌细胞凋亡情况及细胞分裂周期各时象的变化。结果 :NDV在体外可使BGC - 82 3胃癌细胞形成明显的细胞病变效应、细胞生长抑制及细胞凋亡 ,且细胞凋亡率与感染时间呈正相关。G2、S期细胞减少 ,增殖指数 (PI)降低 ,与阴性对照组比较有显著性差异 (P<0 .0 1)。结论 :NDV具有显著的抗BGC - 82 3胃癌细胞活性。     

计算机感染病毒的判断方法及防护手段

伴随着计算机技术的发展,计算机病毒的影响也日益扩大。计算机病毒威胁着计算机的安全,也给我们的生活带来了不必要的麻烦。掌握判断计算机是否感染病毒和计算机病毒的防护方法显的尤为重要。     

Proapoptotic and pronecrosis effect of different truncated hepatitis C virus core proteins

Objective: To study the roles of different truncated hepatitis C virus (HCV) core proteins (CORE) in the pathogenesis of HCV persistent infection and hepatocellular carcinoma (HCC) and to assess intracellular localization in transiently transfected cells. Methods: Seven truncated CORE-GFP (green fluorescent protein) fusion protein expression plasmids were constructed,which contained HCV CORE sequences derived from tumor tissues (BT) and non-tumor tissues (BNT) from one patient infected with HCV. Amino acid (aa) lengths were BT: 1-172 aa, 1-126 aa, 1-58 aa, 59-126 aa, 127-172 aa; BNT: 1-172 aa and C191:1-172 aa respectively. Subcellular localization of CORE-GFP was analyzed by con-focal laser scanning microscope. Apoptosis and necrosis were quantified by flow cytometry. Results: Different truncated CORE-GFP localized mainly in the cytoplasm, but nuclear staining was also observed. HCV CORE could induce apoptosis and necrosis, and different truncated COREs could induce cell apoptosis and necrosis at different levels. Among the same length 1-172 aa of BT, BNT and C191, the cell apoptosis and necrosis percentage of BT is highest, and C191 is the lowest (BT＞BNT＞C191). To the different fragment COREs of BT,N-terminal of CORE induced apoptosis and necrosis higher, compared with that of C-terminal (1-172 aa＞1-126 aa＞1-58aa＞ 127-172 aa＞59-126 aa). Conclusion: These results suggest HCV CORE could induce apoptosis and necrosis of cells, which might play an important role in the pathogenesis of HCV persistent infection and HCC and the different CORE domains of different HCV quasi-species might have some difference in their pathogenesis.     

Expression of a begomoviral DNAβ gene in transgenic Nicotiana plants induced abnormal cell division

An increasing number of monopartite begomoviruses are being identified that a satellite molecule (DNAβ) is required to induce typical symptoms in host plants. DNAβ encodes a single gene (termed βC1) encoded in the complementary-sense. We have produced transgenic Nicotiana benthamiana and N. tabacum plants expressing theβC1 gene of a DNAβ associated with Tomato yellow leaf curl China virus (TYLCCNV), under the control of the Cauliflower mosaic virus 35S promoter. Transgenic plants expressing βC1 showed severe developmental abnormalities in both species. Microscopic analysis of sections of both transgenic and non-transgenic N. tabacum leaves showed abnormal outgrowths of transgenic N. tabacum to be due to disorganized cell division (hyperplasia) of spongy and palisade parenchyma. Immuno-gold labeling of sections with a polyclonal antibody against the βC1 protein showed that the βC1 protein accumulated in the nuclei of cells. The possible biological function of the βC1 protein was discussed.     

计算机病毒及其防治

随着计算机技术的发展和互联网使用的普及,计算机已成为人们生活和工作中所依赖的重要工具,可是越来越猖獗的计算机病毒严重威胁计算机的安全。本文详尽地阐述了计算机病毒的特点和具体症状,最后提出一些防治计算机病毒的方法。