荧光光谱法研究间-硝基苯胺与牛血清白蛋白的相互作用

文章采用荧光光谱技术研究了在不同的酸度和温度条件下,间-硝基苯胺与牛血清白蛋白间的相互作用机制.采用荧光猝灭法讨论了不同pH条件下邻硝基苯胺与牛血清白蛋白间的猝灭类型,计算了不同温度和pH条件下的结合常数,并根据热力学参数确定了其主要结合作用力的类型.研究结果表明,间-硝基苯胺对牛血清白蛋白有较强的荧光猝灭作用,符合静态猝灭机理,二者之间的结合作用力主要是范德华力.     

Preparation and adsorption behaviors of Cu(II) ion-imprinted polymers

Imprinted polymers were prepared for selective removal of Cu（Ⅱ） ions from metal solutions. Three ion-imprinted polymers were synthesized with methacrylic acid （MAA）, acrylamide （AA） and N,N＇-methylenebisacrylamide （MBAA） respectively as the functional monomers, ethleneglycoldimethacrylate （EGDMA） as the cross-linking agent, 2,2＇- azobisisobutyronitrile （AIBN） as the initiator and Cu （Ⅱ） ion as the imprint ion. The template Cu （Ⅱ） ion was removed from the polymer by leaching with a liquid of a 1：1 volumetric ratio of HCl to ethylenediaminetetraacetic acid （EDTA）. The capacity and selectivity of Cu（Ⅱ） ion adsorption were investigated with the three imprinted polymers and their non-imprinted counterparts. The polymers have a maximum adsorption capacity at pH 7.0. The isotherm of their batch adsorption of Cu（Ⅱ） ions shows a Langmuir adsorption pattern. Imprinted polymers all have a much higher capacity and higher selectivity of Cu（Ⅱ） adsorption than nonimprinted ones. MAA polymer benefits the most from imprinting. Imprinted MAA polymer has the highest selectivity when used to rebind Cu （Ⅱ） ion from an aqueous solution in the presence of other metal ions. Ion imprinting can be a promising technique of preparing selective adsorbents to separate and preconcentrate metal in a medium of multiple competitive metal ions through solid phase extraction （SPE）.     

杭白菊与黄山贡菊中微量元素的测定

用原子吸收光谱法对杭白菊（Chrysanchemum morifolitm Ramat）及黄山贡菊（Dendranthemamor folium）的Zn,Mn,Cu,Mg和Ph等微量元素进行了测定分析．该方法的标准曲线相关系数为0．99554～0．99986,相对标准偏差（RSD）小于2％．结果表明：杭白菊与黄山贡菊中Fe,Ca,Mg的含量均较高,黄山贡菊Ca,Zn,Cu,Mn含量较高,尤其是Mn．测定水洗后菊花和未水洗菊花中的微量元素含量可为人们日常饮用菊花的方式提供依据．     

Mass spectrometry-based protein-protein interaction techniques and their applications in studies of DNA damage repair

Proteins are major functional units that are tightly connected to form complex and dynamic networks.These networks enable cells and organisms to operate properly and respond efficiently to environmental cues.Over the past decades,many biochemical methods have been developed to search for protein-binding partners in order to understand how protein networks are constructed and connected.At the same time,rapid development in proteomics and mass spectrometry(MS)techniques makes it possible to identify interacting proteins and build comprehensive protein-protein interaction networks.The resulting interactomes and networks have proven informative in the investigation of biological functions,such as in the field of DNA damage repair.In recent years,a number of proteins involved in DNA damage response and DNA repair pathways have been uncovered with MS-based protein-protein interaction studies.As the technologies for enriching associated proteins and MS become more sophisticated,the studies of protein-protein interactions are entering a new era.In this review,we summarize the strategies and recent developments for exploring protein-protein interaction.In addition,we discuss the application of these tools in the investigation of protein-protein interaction networks involved in DNA damage response and DNA repair.     

吸收历史优良传统,促进廉政文化建设

新世纪的廉政建设任重而道远，制度上固然需要保证，而文化上同时也要有保证。廉政文化是通过文化的自身规律和渠道、文化传媒的方式和魅力影响着社会，因此在吸取中国传统道德精华的基础上大力推动廉政文化建设既势在必行，又意义深远。     

矿化垃圾吸附亚甲基蓝实验研究

采用矿化垃圾筛对亚甲基蓝溶液进行了吸附实验研究,考察了矿化垃圾用量、pH值、初始亚甲基蓝浓度、吸附时间等因素对其吸附效果的影响。实验结果表明,矿化垃圾能对染料模拟废水亚甲基蓝溶液有较为理想的脱色效果。该技术投资运行成本低,且原料在国内存在数量多,具有充足的资源与开发利用前景。     

考虑管间液滴形成和下落过程的吸收器模拟及分析

针对以溴化锂水溶液为工质的水平管吸收器，考虑管底部液滴形成和管间下落过程，建立了描述管间滴状形成和下落吸收过程传热传质耦合数学模型。根据数值计算结果，分析了溶液温度、浓度和吸收速率沿管排不同位置和水平管管间的变化，分析了管间距对吸收器整体传热传质性能的影响。结果表明，滴状吸收过程约占总吸收量的30%。与实验数据的对比说明提出的数学模型是合理的。     

Study on arsenic level in ground water of Delhi using hydride generator accessory coupled with atomic absorption spectrophotometer

Surveillance of drinking water is essentially a health measure intended to protect the public from water borne diseases. Hydride generator accessory coupled with atomic absorption spectrophotometer was used to analyze arsenic level in 49 ground water samples collected from different areas of Delhi. Arsenic level in ground water samples was in the range of 0.0170 to 0.100 ppm (Mean-0.0431, Standard Deviation-0.0136, Std. error of Mean-0.00194) with minimum concentration at Raney Well No. 7 (0.0170 ppm) and maximum at Kotla Mubarak Pur (0.100 ppm). Arsenic containing sediments and percolation of chemicals into soil as the result of dumping of garbage rich in chemicals into open landfills could be the possible source of arsenic in ground water of Delhi. Extensive survey and continuous monitoring is required to be made to assess the magnitude of problem and earlier intervention.     

电镀工业氮氧化物废气治理技术

本介绍了氮氧化物废气的来源、危害以及电镀工业氮氧化物排放特点，着重介绍了治理氮氧化物废气的几种方法，并对吸收液和吸收塔进行了深入的探讨和研究。结果表明，使用碱性溶液作为作为吸收液．使用旋流板塔作为吸收设备是治理电镀工业氮氧化物废气的一种较为成熟、可靠的技术。     

树型蕨类桫椤生物学特征初探

植物要长成大树需要支持能力、水分供给能力和生物积累量.桫椤茎的下端着生大量不定根形成的＂根被＂和茎共同维持它的支持能力和水分供给能力,而它的大型树冠产生的生物积累量也使桫椤长成大树成为可能.