离子极化与键型关系的研究

离子极化学键型有着重要的影响，结合卤素进行了探讨．     

Towards skin polarization characterization using polarimetric technique

Measurement of optical properties of skin is an expanding and growing field of research. Recent studies have shown that the biological tissue, especially skin, changes the polarization state of the incident light. Using this property will enable the study of abnormalities and diseases that alter not only the light intensity but also its polarization state. In this paper we report an experimental study for measuring changes of polarization state of the light scattered from a phantom similar to a sample model of scattering skin. Using the notation of Stokes vector for the polarized light and Mueller matrix for the sample with its polarization properties, we have shown that some elements of the matrix were particularly sensitive to the changes of the polarization-altering physical properties of the scatterers within the phantom.      

成渝城市群创新极化演进及动因分析

为了对我国城市群创新极化问题的演变过程及影响因素进行由表及里地梳理,以成渝城市群为研究区域,以其内部16个城市2010—2019年间的授权专利量为研究变量,分别运用自然断裂法、创新极化贡献度模型以及地理加权回归(GWR)模型对该区域的创新极化问题从现状及演变过程、各城市极化贡献度以及影响因素3个层面进行分析,得到区域创新节点的创新能力(产出)与其对整体创新极化贡献程度并非严格对等的结论。结果显示:成渝城市群总体创新产出明显提升但极化问题严重,有15座城市呈现出创新产出收敛、1座城市创新产出发散,创新极化贡献度的地理分布大致呈外围高、中间低的环状;5类影响创新产出的因子呈现较为稳定的空间分布,且峰值都出现在成都和重庆,其中政府支持、经济水平发挥了主导性作用,而流动性的影响则相对有限。在此基础上,对成渝城市群创新极化问题的解决尝试性地提出发挥核心城市引领作用、加快区域一体化发展,构建全面合作框架、建设内部创新资源数据库与分享平台等对策建议。     

荧光定量PCR对微小RNA的检测及应用

目的:建立一种定量、简单、快速、敏感的微小RNA(miRNA,miR)检测方法,并用其观察一些病理状态下有关miRNA的变化.方法:通过特殊设计的茎环结构的反转录引物,对miRNA进行反转录,然后用相应miRNA的正反向引物对该miRNA进行定量检测,观察重复性和特异性.用此法检测了人巨细胞病毒(HCMV)对滋养细胞(HPT-8)细胞内miR-513表达水平的影响,也检测了结直肠癌组织miR-145水平.结果:用该法扩增miRNA特异性强,结果重复性好,该法检测到HCMV感染对miR-513表达的梯度效应.也发现癌组织和正常组织间miR-145的表达有明显的差异.结论:用荧光定量PCR可以对微小RNA快速有效的检测,在临床工作中可得到很好的应用.     

From perceived polarization of immigration attitudes to collective action

In the context where immigration divides the political space of Western societies, perceived social polarization as an explanation of collective action is surprisingly understudied in contemporary social psychology. We hypothesize that the more people perceive polarization, the more they will engage in collective action in line with their attitudes. Moreover, this effect should be explained by two interrelated factors: identification and perceived efficacy. Perceived polarization should shape how important immigration attitudes are for individuals’ self-definition and thereby believing oneself capable of making a change, which in turn triggers collective action. To test our predictions, we conducted three studies (Studies 1 and 2 were correlational and Study 3 experimental) among mobilized and non-mobilized samples in two countries (i.e., Belgium and Switzerland). Results partially support our predictions that perceived social polarization on immigration issues relates to engaging in collective action. Indirect effect analyses revealed the predominant role of identity dynamics in the social psychological processes linked to perceiving polarization. These results provide potential explanations to the strong mobilization that emerged since 2015 following the so-called migrant crisis. Implications of our findings for collective action literature are discussed.     

Binding interactions of pefloxacin mesylate with bovine lactoferrin and human serum albumin

The binding of pefloxacin mesylate （PFLX） to bovine lactoferrin （BLf） and human serum albumin （HSA） in dilute aqueous solution was studied using fluorescence spectra and absorbance spectra. The binding constant K and the binding sites n were obtained by fluorescence quenching method. The binding distance r and energy-transfer efficiency E between pefloxacin mesylate and bovine lactoferrin as well as human serum albumin were also obtained according to the mechanism of Forster-type dipole-dipole nonradiative energy-transfer. The effects of pefloxacin mesylate on the conformations of bovine lactoferrin and human serum albumin were also analyzed using synchronous fluorescence spectroscopy.     

钝顶螺旋藻藻胆体在解离过程中液氮(77K)荧光发射光谱与光能传递的研究

对钝顶螺旋藻藻胆体在解离过程中77K荧光发射光谱与光能传递途径进行了研究.在77K荧光发射光谱中,完整藻胆体只有一个峰,位于682nm,这是藻胆体核心-类囊体膜连接多肽的荧光峰;随着藻胆体逐渐解离,首先在649nm出现一个小峰,之后,在661nm又出现一个荧光发射肩,前者是C-藻蓝蛋白的荧光,后者是别藻蓝蛋白的荧光;随着藻胆体解离程度的加重,C-藻蓝蛋白的荧光逐渐加强并成为主峰,别藻蓝蛋白的荧光也逐渐加强,但随后又逐渐减弱,直至消失;682nm主峰逐渐减弱并消失,但在679nm却出现另一个荧光峰,这是别藻蓝蛋白-B的荧光;当别藻蓝蛋白的荧光消失后,679nm荧光峰减弱为荧光发射肩,但仍然有很强的荧光.这表明:C-藻蓝蛋白不但与别藻蓝蛋白相连接,而且还与别藻蓝蛋白-B相连,在钝顶螺旋藻藻胆体中,光能是沿着两条途径传递给光系统Ⅱ的.     

Three-dimensional tracking of GLUT4 vesicles in TIRF microscopy

TIRF microscopy has provided a means to view mobile granules within 100 nm in size in two dimensions. However quantitative analysis of the position and motion of those granules requires an appropriate tracking method. In this paper, we present a new tracking algorithm combined with the unique features of TIRF. Firstly a fluorescence correction procedure was processed to solve the problem of fluorescence bleaching over time. Mobile granules were then segmented from a time-lapse image stack by an adaptive background subtraction method. Kalman filter was introduced to estimate and track the granules that allowed reducing searching range and hence greater reliability in tracking process. After the tracked granules were located in x-y plane, the z-position was indirectly inferred from the changes in their intensities. In the experiments the algorithm was applied in tracking GLUT4 vesicles in living adipose cells. The results indicate that the algorithm has achieved robust estimation and tracking of the vesicles in three dimensions.     

英语教学中两极分化问题及其解决

两极分化问题是中学英语教学中极易出现的问题，其产生的原因是多方面的。但是，只要充分发挥教师的主导作用及学生的主体作用，实现教和学的统一，两极分化问题是可以得到解决的。