HPLC-荧光法测定中成药中厚朴酚与和厚朴酚含量

建立了一种用高效液相色谱(HPLC)荧光检测法同时测定中成药中厚朴酚与和厚朴酚含量的新方法.使用BDSC18(100mm×4.0mm,i.d.,3μm)色谱柱,流动相为乙腈0.3%-H3PO4(50∶50,V/V),荧光激发波长为220nm,发射波长为380nm.厚朴酚在0.08～246.0μg/mL范围内线性关系良好,检出限为18.4ng/mL,平均回收率为90.2%￣102.7%,日内RSD为0.80%～4.36%,日间RSD为2.18%～5.92%(n=5);和厚朴酚在0.1～231.0μg/mL范围内线性关系良好,检出限为8.7ng/mL,平均回收率为90.0%～98.1%,日内RSD为0.43%～5.53%,日间RSD为3.97%～6.50%(n=5).该法灵敏度高、选择性好、简便、易重复,为香砂养胃丸、藿香正气软胶囊、国公酒中厚朴酚与和厚朴酚的含量测定提供了有效方法.     

Social Contradictions in Informational Capitalism: The Case of Finnish Wage Earners and Their Labor Market Situation

Along with the diffusion of information and communication technologies (ICTs), work processes are becoming ever more knowledge intensive. In keeping with this trend, the number of informational (or knowledge) workers in Finland has more than tripled from 12% in 1988 to 39% in 2000. What makes the Finnish case unique and interesting is the exceptional speed with which the information sector of the economy has grown. A few years after facing the most severe economic recession in its history in the early 1990s, Finland is now considered to have an advanced information economy. However, our empirical analysis--based on survey data from 1988, 1994, and 2000--yields a somewhat more critical picture of the Finnish information society than what usually comes across in the mainstream media. The opportunities for social equality offered by the growth of informational work are far more limited than was the case with the transition from agricultural to industrial production.     

Determination of fluorinated quinolone antibacterials by ion chromatography with fluorescence detection

For preparing fluorinated quinolone antibiotic medicine locally used in stomatology, simultaneous determination of norfloxacin, ciprofloxacin, and enoxacin was carried out by multiphase ion chromatography with fluorescence detection. Quinolone antibiotics were separated by Dionex OmniPac PAX-500 column with an eluent of 15 mmol/L H2SO4 and 35% methanol （v/v） at a flow-rate of 1.0 ml/min and detected with fluorescence with excitation and emission wave lengths of 347 ran and 420 ran respectively. The detection limits （S/N=3） of norfloxacin, ciprofloxacin and enoxacin were 50, 105 and 80 ng/ml respectively. The relative standard deviations of retention time, peak area and peak height were less than 1.1% and good linear relationship resulted. The developed method was applied to pharmaceutical formulations and biological fluids.     

浅议石油管道腐蚀实时在线监测系统

文章通过对挂片失重法、实时在线监测技术在油田生产系统中的应用研究,明确了油田生产系统各环节的腐蚀状况、特征及变化趋势,为腐蚀的防护提供了科学依据,为防腐措施的效果评价奠定了基础,达到了腐蚀监测目的.     

Cu（Ⅱ）与牛血清白蛋白相互作用的荧光光谱研究

利用荧光光谱技术,从分子水平上研究了模拟生理条件下Cu2＋与牛血清白蛋白（BSA）的相互作用机理。实验结果表明,Cu2＋使得内部疏水区域的色氨酸（Trp）等芳香环氨基酸逐步暴露出来,在荧光光谱中表现为BSA内源荧光的猝灭,猝灭机理主要为静态猝灭。Cu2＋与BSA的结合常数为8.73×104,结合比为1：1。     

苔藓植物光合作用荧光光谱和动力学荧光的比较

本文首次比较了光合作用的荧光光谱和荧光动力学在苔藓植物的原始种类和进化种类之间的异同。原始的和进化的苔藓植物具有发射波长相同的室温荧光光谱，其发射高峰位于686-690nm(来自光系统Ⅱ)和736-740nm(来自光系统Ⅰ)。  而它们的低温(77K)荧光光谱有三个发射峰：F687-689和F697-699来自光系统Ⅱ，F723-734来自光系统I。 前两个峰在原始的和进化的种类中基本相同。  按第三个发射峰可把被测的苔藓植物分为两组：发射峰在725nm左右的有细牛毛藓、长肋对齿藓、对齿藓、斜叶芦荟藓、密集匍灯藓和地钱，它们是较原始的藓类和较进化的苔类；发射峰在732nm左右的有细枝羽藓、东亚金灰藓、鼠尾藓、鳞叶藓、粗枝藓和美灰藓等较进化的藓类，也有较原始的钝叶匍灯藓。已知光系统I核心复合物CPI的77K荧光发射峰在722nm，而CPIa(核心复合物与外周天线复合物)和LHC-I(外周天线复合物)的发射峰在730nm。这说明在苔藓植物进化过程中，光系统Ⅱ比较保守；而光系统Ⅰ有所变化，原始的藓类主要含有光系统Ⅰ核心复合物，而较进化的藓类才含有较完善的外周天线复合物。光合作用荧光动力学分析表明，在原始藓类和地钱中具有较低的光系统Ⅱ活性、光系统Ⅱ的原初光能转换效率、光合碳同化和潜在的光合量子转换效率；而较进化的具有较高的活性和效率。 但是，原始的密集匍灯藓也具有较高的活性和效率，而进化的美灰藓却具有较低的活性和效率。这可能表明这两种植物是由原始向进化发展过程中的中间类型。     

荧光的二阶相干性研究

借助相干性等概念的阐述,结合荧光的产生机理、性质以及量子力学的相关原理,揭示了荧光的二阶相干性、相干函数及其相关性质,并分析讨论了它们之间的关系,     

太赫兹波段二维金属光子晶体传输器件的研究

运用频域有限元法研究了金属铜介质柱构成的二维光子晶体传输特性。通过改变铜柱形状和大小,研究了晶格常数为200μm的正方晶格光子晶体的TM模、TE模的禁带特性。选取金属铜为介质柱,空气为背景材料,对正方铜柱与旋转45度正方铜柱进行了大量计算与分析。结果表明,传输特性随着介质柱形状和填充率的变化而变化,且TE模和TM模带隙差异很大,波段并不相同,这为THz波段的光子晶体滤波器、反射器、极化器的开发与制作提供了理论依据。     

Synergistic interaction of NaCl and Cd on growth and photosynthetic parameters in soybean genotypes differing in salinity tolerance

The effects of salinity(50 mmol/L NaCl) and Cd(1 μmol/L CdCl2) as sole and combined on growth and photosyn-thetic parameters were studied using two soybean genotypes,Huachun 18 and NGB.The concentrations of Cd2 ,Zn2 ,Ca2 ,Mg2 ,K and Na were also determined in seeds and pods.Huachun 18 suffered a more serious decrease than NGB in net photosynthetic rate(Pn) in the treatments of salinity stress alone and combined stress(NaCl Cd),showing that it is relatively sensitive to salinity.The decrease in Pn caused by salt stress in Huachun 18 was mainly due to the reduced total chlorophyll content and photosynthetic efficiency(the ratio of variable fluorescence to maximal fluorescence,Fv/Fm),whereas the decease in NGB was mainly related to reduced stomatal conductance(Gs).The combined stress of both Na and Cd did not induce further decrease in photosynthesis and fluorescence in the two genotypes relative to salt or Cd stress alone.Greater change in the pod concentrations of Zn2 ,Ca2 ,Mg2 ,K and Na was detected under salt stress for Huachun 18 than for NGB.The results suggested that the interactive effect of NaCl-Cd on growth and nutrient uptake differs between the two soybean genotypes.