人造生命技术及其伦理学讨论综述

生命科学的迅猛发展不可避免地涉及人类自身,随着后基因组时代的到来,合成生物学、人造生命技术的发展必将广泛地应用于能源、环境、材料、医药等诸多领域,因此科学技术进步与传统伦理之间的矛盾也必将出现,从而影响和改变人类未来的生活。     

HiHiLo对心功能的影响与ACE基因I/D多态性的关联研究

目的:探讨低氧训练对心脏结构与功能的影响及其与中国人群ACE基因I/D多态性之间的关联性.方法:76名中国北方平原地区汉族男子进行4周高住高练低训( HiHiLo),方案为每日在低氧环境(O2浓度为14.5％～14.8％)中休息10h,每周进行3次75％VO2 max低氧训练(O2浓度为15.4％),其余时间在常氧下训练;HiHiLo前后测定左心室结构与功能相关指标;PCR分析受试者的ACE基因I/D多态性.结果:HiHiLo后心脏结构指标LVM、LVMI、IVSD和PWD都显著性增加(P＜0.05);训练后安静时、各种负荷时和恢复时EDV、EF、SV、SVI和E/A显著性增加(P＜0.05),HR、CO、COI、Vmn和Vp显著性减小(P＜0.05).HiHiLo前各指标在ACE各基因型间无显著性差异(P＞0.05);除50 W和150W时HR外,其余心脏结构和功能指标训练后变化在各组间均未见显著性差异(P＞0.05).结论:HiHiLo能使左心室结构和功能呈现良好的顺应性变化;ACE基因型与个体心功能对HiHiLo的敏感性无关.     

二化螟隐花色素基因cryptochrome1和cryptochrome2的克隆与表达谱分析

隐花色素基因(cryptochrome,简称cry)是一类生物钟基因,参与生物体昼夜节律调控。本实验克隆了二化螟2个隐花色素基因,分别命名为Cs-cry1和Cs-cry2(Genbank登录号分别为HG780135和KF977409),其分别包含1605 bp和2289 bp的开放阅读框,分别编码由534和762个氨基酸组成的蛋白;Cs-cry1与其它鳞翅目昆虫cry1的相似性较高,而Cs-cry2与其它鳞翅目昆虫cry2的相似性较高,这与系统进化分析结果相一致;半定量RT-PCR研究表明Cs-cry1和Cs-cry2基因在二化螟成虫触角、头、雄、腹、足和翅等不同组织中均有表达。实验结果为二化螟昼夜节律分子机制研究提供了理论依据。     

高校《外国文学》教学模式的探析

《外国文学》是一门重要的课程,开拓学生的视野,提高学生的审美能力,让学生在审美中得到教育,获得道德的提升,这是目标之所在。针对目前《外国文学》课程教学不容乐观的现状,教师采用的教学内容和手段以及学生的学习态度都有提升的空间。通过探索合理的教学模式,以生动的讲解为核心,辅之以双语教学、多媒体、讨论法和比较法,效果显著,具有一定的推广和借鉴价值。     

基于.NET的外语自主学习系统的设计与实现

外语网络自主学习已成为提高学生外语应用能力不可或缺的手段,为弥补学校无自主研发外语自主学习系统的缺陷,我们研发了基于.NET的软件。本文就该系统研发过程及研究结果进行阐述,讨论了自主学习系统的重要性,对需求进行分析并设计系统功能板块及数据库,从前台和后台方面分别论述系统的实现过程,最后对该系统进行归纳总结。     

Transcriptomic Analysis of Aflatoxin B1-Regulated Genes in Rat Hepatic Epithelial Cells

Aflatoxins are the most popular hepatotoxicants. Chronic exposure to aflatoxins leads to a wide variety of liver diseases, such as hepatocellular carcinoma. In this study, we analyzed the genome wide expression profiles of aflatoxin B1-induced rat hepatic epithelial cells. The expression of 325, 184 and 199 special genes was altered when exposed to 0.03, 0.1 and 0.2 μmol/L aflatoxin B1 respectively, and 239 genes were commonly expressed. After the functional analysis on these dose-special genes, we determined several key pathways related to hepatotoxicity, such as TGF-beta signaling pathway, tight junction, adherens junction, the regulation of actin cytoskeleton, Erb B signaling pathway, p53 signaling pathway, pathways in cancer and axon guidance. Common genes were mainly associated with focal adhesion, ECM-receptor interaction, and cell adhesion molecules. Gene ontology annotations showed a good concordance with these pathways. The quantitative real-time polymerase chain reaction(PCR) analysis of selected genes showed similar patterns in microarrays. The toxicogenomic study provides a better understanding of molecular mechanisms of aflatoxins.     

徽州文化的基因、特质与解构

徽州文化是代表中国明清社会从“农业的系统”向“商业的系统”转变之际的特异性与先导性的地域文化标本。徽州文化的独特性取决于它的文化基因,包括移民型人口结构、宗法制社会结构、商贾为重的经济结构与崇儒尊孔奉朱的精神结构。徽州文化的特质是既脱胎于中华文化母体,又成为新兴商业文化的先驱者,成为新旧文化二律背后的共存体,表现出许多彼此矛盾又自我调谐的文化表征,其中最典型的为：社会价值与人生价值观上的崇儒与重商,人生态度上的内敛与进取,生活方式上的节俭与时尚。徽州文化解构、降格为普通地域文化,最根本、最关键的动因是构成徽文化特质的内在基因产生了变异：移民型人口结构发生反向移民,宗法制社会结构彻底瓦解,以商贾为重的经济结构被迫转型,崇儒尊孔奉朱的精神结构逐渐涣散,而这种种基因的嬗变受制于内外环境的变革压力。     

Chinese TEFL teachers’ perceptions about research and influences on their research endeavours

English has always occupied a key position in China’s education. The quality of English education depends largely on the quality of the English teaching force. Improving the overall quality of Teaching English as a Foreign Language (TEFL) teachers entails advancing both their teaching and research competence. This study, with its focus on Chinese TEFL teachers working in a higher education institution, was set up in a context where Chinese higher education colleges are being transformed into universities and research is becoming a crucial aspect of all teachers’ work. This small-scale case study investigated a group of Chinese TEFL teachers’ perceptions about research and their individual and workplace characteristics that influenced their research endeavours. The findings revealed that Chinese TEFL teachers recognised the significance of research for teaching, professional growth and career advancement. However, lack of individual characteristics such as research and disciplinary knowledge, confidence in research and intrinsic motivation impeded their research efforts. Their institution and departments seemed to encourage research; yet, more specific financial and academic support to start and sustain their research endeavours is required. This study’s findings provide implications for both individual teachers and their institutions to engage TEFL teachers more actively in research.     

Deregulation of microRNA expression in thyroid tumors简

MicroRNAs (miRNAs or miRs) are endogenous non-coding RNAs that negatively regulate gene expression by binding to the 3′ non-coding regions of target mRNAs, resulting in their cleavage or blocking their translation. miRNAs may have an impact on cell differentiation, proliferation, and survival, and their deregulation can be inclined to diseases and cancers, including thyroid tumors. The purpose of this review is to summarize the existing findings of deregulated miRNAs in different types of thyroid tumors and to exhibit their potential target genes, especially to demonstrate those involved in tumor invasion and metastasis. In addition, new findings of circulating miRNA expression profiles, single nucleotide polymorphism (SNP) in thyroid tumors, and the correlation of somatic mutations with deregulated miRNA expression in thyroid tumors were all included in this review.     

提高留学生神经外科学临床教学质量的探索

为了提高医学留学生神经外科学临床教学质量,苏州大学附属第二医院神经外科严格筛选带教教师,加强师资队伍的建设,探索创新型带教模式,针对留学生制订个体化的因材施教的培养方案,从中凝练出有益的教学启发与经验,并为今后留学生神经外科学临床带教工作提出了改进的方向。